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Identification of an ATP-sensitive potassium channel in mitochondria
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Journal Article
Identification of an ATP-sensitive potassium channel in mitochondria
2019
Overview
Mitochondria provide chemical energy for endoergonic reactions in the form of ATP, and their activity must meet cellular energy requirements, but the mechanisms that link organelle performance to ATP levels are poorly understood. Here we confirm the existence of a protein complex localized in mitochondria that mediates ATP-dependent potassium currents (that is, mitoK
ATP
). We show that—similar to their plasma membrane counterparts—mitoK
ATP
channels are composed of pore-forming and ATP-binding subunits, which we term MITOK and MITOSUR, respectively. In vitro reconstitution of MITOK together with MITOSUR recapitulates the main properties of mitoK
ATP
. Overexpression of MITOK triggers marked organelle swelling, whereas the genetic ablation of this subunit causes instability in the mitochondrial membrane potential, widening of the intracristal space and decreased oxidative phosphorylation. In a mouse model, the loss of MITOK suppresses the cardioprotection that is elicited by pharmacological preconditioning induced by diazoxide. Our results indicate that mitoK
ATP
channels respond to the cellular energetic status by regulating organelle volume and function, and thereby have a key role in mitochondrial physiology and potential effects on several pathological processes.
The pore-forming and ATP-binding subunits of a mitochondrial protein complex that mediates ATP-dependent potassium currents are identified and characterized, revealing the role of this channel in mitochondrial physiology and pathologies.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/109
/ 14/19
/ 14/35
/ Ablation
/ Adenosine Triphosphate - metabolism
/ Animals
/ Cardiotonic Agents - pharmacology
/ Channels
/ Electrophysiological Phenomena
/ Heart
/ Humanities and Social Sciences
/ Ischemic Preconditioning, Myocardial
/ Male
/ Membrane Potential, Mitochondrial
/ Mice
/ Mitochondria, Heart - drug effects
/ Mitochondria, Heart - metabolism
/ Mitochondria, Heart - pathology
/ Mitochondria, Heart - physiology
/ Potassium Channels - chemistry
/ Potassium Channels - metabolism
/ Protein Subunits - chemistry
/ Protein Subunits - metabolism
/ Proteins
/ Science
