Asset Details
Do you wish to reserve the book?
Genomic characterization and probiotic potential assessment of an exopolysaccharide-producing strain Pediococcus pentosaceus LL-07 isolated from fermented meat
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Genomic characterization and probiotic potential assessment of an exopolysaccharide-producing strain Pediococcus pentosaceus LL-07 isolated from fermented meat
Do you wish to request the book?
Genomic characterization and probiotic potential assessment of an exopolysaccharide-producing strain Pediococcus pentosaceus LL-07 isolated from fermented meat
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Genomic characterization and probiotic potential assessment of an exopolysaccharide-producing strain Pediococcus pentosaceus LL-07 isolated from fermented meat
2024
Overview
Background
The genomic information available for
Pediococcus pentosaceus
is primarily derived from fermented fruits and vegetables, with less information available from fermented meat.
P. pentosaceus
LL-07, a strain isolated from fermented meat, has the capability of producing exopolysaccharides (EPS). To assess the probiotic attributes of
P. pentosaceus
LL-07, we conducted whole-genome sequencing (WGS) using the PacBio SequelIIe and Illumina MiSeq platforms, followed by in vitro experiments to explore its probiotic potential.
Results
The genome size of
P. pentosaceus
LL-07 is 1,782,685 bp, comprising a circular chromosome and a circular plasmid. Our investigation revealed the absence of a CRISPR/Cas system. Sugar fermentation experiments demonstrated the characteristics of carbohydrate metabolism.
P. pentosaceus
LL-07 contains an EPS synthesis gene cluster consisting of 13 genes, which is different from the currently known gene cluster structure. NO genes associated with hemolysis or toxin synthesis were detected. Additionally, eighty-six genes related to antibiotic resistance were identified but not present in the prophage, transposon or plasmid. In vitro experiments demonstrated that
P. pentosaceus
LL-07 was comparable to the reference strain
P. pentosaceus
ATCC25745 in terms of tolerance to artificial digestive juice and bile, autoaggregation and antioxidation, and provided corresponding genomic evidence.
Conclusion
This study confirmed the safety and probiotic properties of
P. pentosaceus
LL-07 via complete genome and phenotype analysis, supporting its characterization as a potential probiotic candidate.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Bacteria
/ Biomedical and Life Sciences
/ CRISPR
/ Drug resistance in microorganisms
/ Enzymes
/ Fermented Foods - microbiology
/ Food
/ Genes
/ Genomes
/ Genomics
/ Humans
/ Meat
/ Mycology
/ Pediococcus pentosaceus - genetics
/ Pediococcus pentosaceus - metabolism
/ Polysaccharides, Bacterial - biosynthesis
/ Polysaccharides, Bacterial - metabolism
/ Software
/ Toxins
/ Virology
