Journal Article

A Synthetic Conjugate Polysaccharide Vaccine against Haemophilus Influenzae Type B

Hardy, Eugenio, Valdés, Yury, Aguilar, Aristides, Toraño, Gilda, Carmenates, Ania, Campa, Concepción, Toledo, Maria E., Rodríguez, Maria C., Verez-Bencomo, V., Garcia, Ernesto, Roy, René, Rodriguez, Arlene, Sosa, Iván, Herrera, Luis, Medina, Ernesto, Izquierdo, Mabel, Cardoso, Félix, Deler, Mercedes L., Fernández-Santana, V., Diaz, Manuel, Martínez, Raydel, Montane, Manuel, Cosme, Karelia, Hernandez, Ibis, Baly, Alberto, Costa, Lourdes, Villar, Annette, Ramos, Alexis, Muzachio, Alexis, Heynngnezz, Lazaro

2004

Overview

Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, however, no commercial vaccine has been available in which the key carbohydrate antigens are produced synthetically. We describe the large-scale synthesis, pharmaceutical development, and clinical evaluation of a conjugate vaccine composed of a synthetic capsular polysaccharide antigen of Haemophilus influenzae type b (Hib). The vaccine was evaluated in clinical trials in Cuba and showed long-term protective antibody titers that compared favorably to licensed products prepared with the Hib polysaccharide extracted from bacteria. This demonstrates that access to synthetic complex carbohydrate-based vaccines is feasible and provides a basis for further development of similar approaches for other human pathogens.

• Publisher: American Association for the Advancement of Science,The American Association for the Advancement of Science
• Subject: Adult / Antibodies / Antibodies, Bacterial - biosynthesis / Antibodies, Bacterial - blood / Antigens / Bacteria / Bacterial diseases / Biological and medical sciences / Carbohydrates / Child, Preschool / Clinical trials / Conjugate vaccines / Developed Nations / Dosage / Double-Blind Method / Drug therapy / Fundamental and applied biological sciences. Psychology / Glycoconjugates / Glycoconjugates - immunology / Haemophilus influenzae / Haemophilus influenzae type b / Haemophilus influenzae type b - immunology / Haemophilus Vaccines - administration & dosage / Haemophilus Vaccines - chemical synthesis / Haemophilus Vaccines - immunology / Hemophilus influenzae / Humans / Immunization Schedule / Immunoglobulin G - blood / Infant / Infections / Manufacturing / Microbiology / Organic Chemistry / Pathogens / Phosphates / Polysaccharides / Polysaccharides - chemical synthesis / Polysaccharides - immunology / Polysaccharides - isolation & purification / Polysaccharides, Bacterial - immunology / Polysaccharides, Bacterial - isolation & purification / Prevention / Prophylaxis / Tetanus Toxoid - immunology / Vaccination / Vaccines / Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies / Vaccines, Conjugate - administration & dosage / Vaccines, Conjugate - immunology / Virology / Young Children