Asset Details
Do you wish to reserve the book?
A microfluidic based biosensor for rapid detection of Salmonella in food products
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
A microfluidic based biosensor for rapid detection of Salmonella in food products
Do you wish to request the book?
A microfluidic based biosensor for rapid detection of Salmonella in food products
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
A microfluidic based biosensor for rapid detection of Salmonella in food products
2019
Overview
An impedance based microfluidic biosensor for simultaneous and rapid detection of Salmonella serotypes B and D in ready-to-eat (RTE) Turkey matrix has been presented. Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has two sensing regions, with each formed from one interdigitated electrode array (IDE array) consisting of 50 finger pairs. First, Salmonella antibody type B and D were prepared and delivered to the sensor to functionalize each sensing region without causing any cross contamination. Then the RTE Turkey samples spiked with Salmonella types B and D were introduced into the biosensor via the antigen inlet. The response signal resulted from the binding between Salmonella and its specific antibody demonstrated the sensor's ability to detect a single type of pathogen, and multiple pathogens simultaneously. In addition, the biosensor's selectivity was tested using non-specific binding of E. coli O157 and E. coli DH5 Alpha while the IDE array was coated with the Salmonella antibody. The results also showed the sensor is capable to differentiate low concentration of live Salmonella cells from high concentration of dead Salmonella cells, and high concentration of E. coli cells. A detailed study on antibody immobilization that includes antibody concentration, antibody coating time (0.5-3 hours) and use of cross-linker has been performed. The study showed that Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after electrodes were saturated with antibody, while the optimal coating time was found to be 1.5 hours, and the use of cross-linker has improved the signal response by 45-60%.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Antibodies, Immobilized - chemistry
/ Antigens
/ Arrays
/ Bacteria
/ Binding
/ Biosensing Techniques - economics
/ Biosensing Techniques - instrumentation
/ Carbon
/ Coatings
/ E coli
/ Food
/ Food Analysis - instrumentation
/ Food Contamination - analysis
/ Identification and classification
/ Medicine and Health Sciences
/ Microfluidic Analytical Techniques - economics
/ Microfluidic Analytical Techniques - instrumentation
/ Research and Analysis Methods
/ Salmonella - isolation & purification
/ Sensors
