Journal Article

Topical Formulation Containing Naringenin: Efficacy against Ultraviolet B Irradiation-Induced Skin Inflammation and Oxidative Stress in Mice

Martinez, Renata M., Baracat, Marcela M., Casagrande, Rubia, Georgetti, Sandra R., Pinho-Ribeiro, Felipe A., Vignoli, Josiane A., Verri, Waldiceu A., Vicentini, Fabiana T. M. C., Steffen, Vinicius S., Caviglione, Carla V., Fonseca, Maria J. V., Silva, Thais C. C., Bottura, Carolina

2016

Overview

Naringenin (NGN) exhibits anti-inflammatory and antioxidant activities, but it remains undetermined its topical actions against ultraviolet B (UVB)-induced inflammation and oxidative stress in vivo. The purpose of this study was to evaluate the physicochemical and functional antioxidant stability of NGN containing formulations, and the effects of selected NGN containing formulation on UVB irradiation-induced skin inflammation and oxidative damage in hairless mice. NGN presented ferric reducing power, ability to scavenge 2,2'-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and hydroxyl radical, and inhibited iron-independent and dependent lipid peroxidation. Among the three formulations containing NGN, only the F3 kept its physicochemical and functional stability over 180 days. Topical application of F3 in mice protected from UVB-induced skin damage by inhibiting edema and cytokine production (TNF-α, IL-1β, IL-6, and IL-10). Furthermore, F3 inhibited superoxide anion and lipid hydroperoxides production and maintained ferric reducing and ABTS scavenging abilities, catalase activity, and reduced glutathione levels. In addition, F3 maintained mRNA expression of cellular antioxidants glutathione peroxidase 1, glutathione reductase and transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2), and induced mRNA expression of heme oxygenase-1. In conclusion, a formulation containing NGN may be a promising approach to protecting the skin from the deleterious effects of UVB irradiation.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Administration, Cutaneous / Analysis / Animals / Antioxidants / Antioxidants (Nutrients) / Antioxidants - pharmacology / Benzothiazoles - antagonists & inhibitors / Benzothiazoles - metabolism / Care and treatment / Catalase / Catalase - genetics / Catalase - metabolism / Citrus fruits / Cytokines / Dermatitis / Dosage and administration / Edema / Edema - etiology / Edema - genetics / Edema - pathology / Edema - prevention & control / Flavanones - pharmacology / Flavonoids / Formulations / Gene expression / Gene Expression - drug effects / Glutathione / Glutathione - metabolism / Glutathione peroxidase / Glutathione Peroxidase - genetics / Glutathione Peroxidase - metabolism / Glutathione reductase / Glutathione Reductase - genetics / Glutathione Reductase - metabolism / Hairless / Heme / Heme oxygenase (decyclizing) / Heme Oxygenase-1 - genetics / Heme Oxygenase-1 - metabolism / Hydroxyl Radical - antagonists & inhibitors / Hydroxyl Radical - metabolism / Hydroxyl radicals / In vivo methods and tests / Inflammation / Interleukin 10 / Interleukin 6 / Interleukin-10 - genetics / Interleukin-10 - metabolism / Interleukin-1beta - genetics / Interleukin-1beta - metabolism / Interleukin-6 - genetics / Interleukin-6 - metabolism / Iron / Irradiation / Life assessment / Lipid peroxidation / Lipid Peroxidation - drug effects / Mice / Mice, Hairless / Mitochondria / Naringenin / NF-E2-Related Factor 2 - genetics / NF-E2-Related Factor 2 - metabolism / Oxidative stress / Oxidative Stress - drug effects / Oxygenase / Peroxidase / Peroxidation / Radiation damage / Risk factors / Rodents / Signal transduction / Skin / Skin - drug effects / Skin - metabolism / Skin - pathology / Skin - radiation effects / Stability analysis / Sulfonic acid / Sulfonic Acids - antagonists & inhibitors / Sulfonic Acids - metabolism / Superoxide / Superoxides - antagonists & inhibitors / Superoxides - metabolism / Topical application / Tumor Necrosis Factor-alpha - genetics / Tumor Necrosis Factor-alpha - metabolism / Tumor necrosis factor-TNF / Ultraviolet radiation / Ultraviolet Rays - adverse effects