Journal Article

Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Harcourt, Jennifer, Tao, Ying, Sakthivel, Senthil K., Goldsmith, Cynthia, Lokugamage, Kumari G., Makino, Shinji, Gautam, Rashi, Menachery, Vineet D., Wang, Haibin, Zhang, Jing, Bullock, Hannah A., Scharton, Dionna, Li, Yan, Plante, Jessica A., Murray, Janna, Paden, Clinton R., Thornburg, Natalie J., Widen, Steven G., Kamili, Shifaq, Tamin, Azaibi, Whitaker, Brett, Tong, Suxiang, Weaver, Scott C., Narayanan, Krishna, Queen, Krista, Wang, Lijuan, Lu, Xiaoyan, Plante, Kenneth S., Schindewolf, Craig, Lynch, Brian, Uehara, Anna, Lindstrom, Stephen, Ksiazek, Thomas G., Mirchandani, Divya

2020

Overview

The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.

• Publisher: U.S. National Center for Infectious Diseases,Centers for Disease Control and Prevention
• Subject: Acids / Animals / Antibiotics / Antibodies / Betacoronavirus - genetics / Betacoronavirus - isolation & purification / Betacoronavirus - physiology / Cell culture / Cell Line / Chlorocebus aethiops / coronavirus / Coronavirus Infections - diagnosis / Coronaviruses / COVID-19 / Disease control / Disease transmission / Expedited / Genome, Viral / Genomes / Humans / Microscopy / Nasopharynx - virology / novel coronavirus disease 2019 / Oropharynx - virology / Pandemics / Pathogens / Pneumonia, Viral - diagnosis / Proteins / Public health / Respiratory diseases / SARS-CoV-2 / Severe acute respiratory syndrome / Severe acute respiratory syndrome coronavirus 2 / Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with 2019 Novel Coronavirus Disease, United States / Vero Cells / Viral Tropism / Virus Replication / Viruses / Washington