Journal Article

QTL mapping of shoot and seed traits impacted by Drought in Barley using a recombinant inbred line Population

Hu, Gongshe, Ajayi, Oyeyemi O., Mahalingam, Ramamurthy, Walling, Jason G., Bregitzer, Phil, Klos, Kathy

2023

Overview

Background With ongoing climate change, drought events are severely limiting barley production worldwide and pose a significant risk to the malting, brewing and food industry. The genetic diversity inherent in the barley germplasm offers an important resource to develop stress resiliency. The purpose of this study was to identify novel, stable, and adaptive Quantitative Trait Loci (QTL), and candidate genes associated with drought tolerance. A recombinant inbred line (RIL) population (n = 192) developed from a cross between the drought tolerant ‘Otis’ barley variety, and susceptible ‘Golden Promise’(GP) was subjected to short-term progressive drought during heading in the biotron. This population was also evaluated under irrigated and rainfed conditions in the field for yields and seed protein content. Results Barley 50k iSelect SNP Array was used to genotype the RIL population to elucidate drought-adaptive QTL. Twenty-three QTL (eleven for seed weight, eight for shoot dry weight and four for protein content) were identified across several barley chromosomes. QTL analysis identified genomic regions on chromosome 2 and 5 H that appear to be stable across both environments and accounted for nearly 60% variation in shoot weight and 17.6% variation in seed protein content. QTL at approximately 29 Mbp on chromosome 2 H and 488 Mbp on chromosome 5 H are in very close proximity to ascorbate peroxidase ( APX ) and in the coding sequence of the Dirigent ( DIR ) gene, respectively. Both APX and DIR are well-known key players in abiotic stress tolerance in several plants. In the quest to identify key recombinants with improved tolerance to drought (like Otis) and good malting profiles (like GP), five drought tolerant RILs were selected for malt quality analysis. The selected drought tolerant RILs exhibited one or more traits that were outside the realms of the suggested limits for acceptable commercial malting quality. Conclusions The candidate genes can be used for marker assisted selection and/or genetic manipulation to develop barley cultivars with improved tolerance to drought. RILs with genetic network reshuffling necessary to generate drought tolerance of Otis and favorable malting quality attributes of GP may be realized by screening a larger population.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Agricultural production / Agriculture / Analysis / ascorbate peroxidase / Ascorbic acid / Barley / Biological diversity / Biomedical and Life Sciences / Chromosome 2 / Chromosome 5 / Chromosome Mapping / Chromosomes / Climate change / Cultivars / Drought / Drought resistance / drought tolerance / Droughts / Environmental aspects / Field study / Food / Food industry / Food supply / Gene mapping / Gene Regulatory Networks / Genes / Genetic aspects / Genetic diversity / Genetic engineering / genetic variation / Genomic analysis / genomics / genotype / Genotypes / Germplasm / Global temperature changes / Golden promise / Growth / Hordeum - genetics / inbred lines / Inbreeding / irrigation / L-Ascorbate peroxidase / Life Sciences / Malt / Malting / Marker-assisted selection / Methods / Morphology / Nutrient content / Otis / Peroxidase / Phenotype / Physiology / Plant heat tolerance / Plant Sciences / Population / protein content / Proteins / Quality management / Quantitative genetics / Quantitative trait loci / Quantitative Trait Loci - genetics / Quantitative trait loci mapping / quantitative traits / Recombinants / risk / seed weight / Seeds / Seeds - genetics / Shoots / single nucleotide polymorphism arrays / Single-nucleotide polymorphism / stress tolerance / Tree Biology / Weight