Journal Article

Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress

Ali, Baber, Iqbal, Majid, Al-Robai, Sami Asir, Ercisli, Sezai, Ullah, Sami, AL-Huqail, Arwa Abdulkreem, Elsaid, Fahmy Gad, Abeed, Amany H. A., Amin, Fazal, Malik, Tabarak, Kaplan, Alevcan, Khan, Muhammad Nauman

2024

Overview

Soil pollution with heavy metals has grown to be a big hassle, leading to the loss in farming production particularly in developing countries like Pakistan, where no proper channel is present for irrigation and extraction of these toxic heavy metals. The present study aims to ameliorate the damages caused by heavy metal ions (Hg-Mercury) on rapeseed ( Brassica napus L.) via a growth regulator (α-tocopherol 150 mg/L) and thermopriming technique at 4 °C and 50 °C to maintain plant agronomical and physiological characteristics. In pot experiments, we designed total of 11 treatments viz.( T0 (control), T1 (Hg4ppm), T2 (Hg8ppm), T3 (Hg4ppm + 4 °C), T4 (Hg4ppm + 4 °C + tocopherol (150 m/L)), T5 (Hg4ppm + 50 °C), T6 (Hg4ppm + 50 °C + tocopherol (150 mg/L)), T7 (Hg8ppm + 4 °C), T8 (Hg8ppm + 4 °C + tocopherol (150 mg/L)), T9 (Hg8ppm + 50 °C), T10 (Hg8ppm + 50 °C + tocopherol (150 mg/L) the results revealed that chlorophyll content at p  < 0.05 with growth regulator and antioxidant enzymes such as catalase, peroxidase, and malondialdehyde enhanced up to the maximum level at T5 = Hg4ppm + 50 °C (50 °C thermopriming under 4 ppm mercuric chloride stress), suggesting that high temperature initiate the antioxidant system to reduce photosystem damage. However, protein, proline, superoxide dismutase at p  < 0.05, and carotenoid, soluble sugar, and ascorbate peroxidase were increased non-significantly ( p  > 0.05) 50 °C thermopriming under 8 ppm high mercuric chloride stress (T9 = Hg8ppm + 50 °C) representing the tolerance of selected specie by synthesizing osmolytes to resist oxidation mechanism. Furthermore, reduction in % MC (moisture content) is easily improved with foliar application of α-tocopherol and 50 °C thermopriming and 4 ppm heavy metal stress at T6 = Hg4ppm + 50 °C + α-tocopherol (150 mg/L), with a remarkable increase in plant vigor and germination energy. It has resulted that the inhibitory effect of only lower concentration (4 ppm) of heavy metal stress was ameliorated by exogenous application of α-tocopherol and thermopriming technique by synthesizing high levels of proline and antioxidant activities in maintaining seedling growth and development on heavy metal contaminated soil.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Agricultural production / Agriculture / alpha-tocopherol / alpha-Tocopherol - metabolism / alpha-Tocopherol - pharmacology / Antioxidant enzymes / Antioxidants / Antioxidants - metabolism / ascorbate peroxidase / Ascorbic acid / Biomedical and Life Sciences / Brassica / Brassica napus / Brassica napus - metabolism / Brassica napus L / carotenoids / Catalase / Chlorides / Chlorophyll / Climate change / Climatic changes / Crops / Damage / Developing countries / energy / Environmental aspects / foliar application / Foliar applications / Food / Germination / Growth / Growth regulators / Heavy metal / Heavy metals / High temperature / Influence / irrigation / L-Ascorbate peroxidase / LDCs / Life Sciences / malondialdehyde / Mercuric chloride / Mercuric Chloride - metabolism / Mercuric Chloride - toxicity / Mercury / Mercury (metal) / Mercury compounds / Metal concentrations / Metal ions / Metals, Heavy - metabolism / Moisture content / Osmolytes / Oxidation / Pakistan / Peroxidase / Photosystem / Plant Sciences / Proline / Proline - metabolism / Rape (Plant) / Rapeseed / seedling growth / Seedlings / Seeds / soil / Soil contamination / Soil Pollutants - metabolism / Soil pollution / Soil stresses / sugars / Superoxide dismutase / Synthesis / Temperature / Tocopherol / Tocopherols - metabolism / Tocopherols - pharmacology / Toxicity / Tree Biology / vigor / Vitamin E / Water content