Journal Article

Effects of Dietary Cytidine 5'-monophosphate on Neu5Gc contents in the Muscle and Viscera of Xiang Pigs

Zhu, Xuling, Xu, Shitao, Li, Hongying, Zhu, Qiujing, Chang, Rui, Hu, Ke, Xu, Aqi, Tang, Pengyu

2020

Overview

In order to reduce the health risks associated with red meat as listed by the World Health Organization (WHO), this paper aimed to elucidate the interaction between cytidine 5'-monophosphate (5'-CMP) supplemented feed and N-glycolylneuraminic acid (Neu5Gc) in experimental animals in vivo. 5'-CMP was added to the diet of 90, 180, and 270 day old Xiang pigs, and after 30 days, the Neu5Gc contents, physicochemical parameters, and free amino acid contents of muscle and internal viscera were measured by high performance liquid chromatography coupled with fluorescence detector (HPLC/FLD). The mechanism by which 5'-CMP affects Neu5Gc contents was investigated by using the molecular docking. The results show that 5'-CMP significantly decreased Neu5Gc content in 180 day old Xiang pigs (P < 0.05), but had no effect on the Neu5Gc contents in 90 and 270 day old Xiang pigs. Umami amino acids were significantly increased in 180 day old Xiang pigs. In the 90 and 270 day old pigs, histidine increased by 10.38% and 17.87%, respectively. The other free amino acids were either reduced or not affected. Moreover, the 5'-CMP diet did not affect the physicochemical parameters of the longissimus muscle in the Xiang pigs. The 5'-CMP could occupy almost all the ST active sites without HIS302 and show inhibition of the ST activity. The results provided an experimental basis for the subsequent reduction of Neu5Gc in red meat before slaughter.