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Decentralizing Recombinant Protein Biomanufacturing Using Cell-Free Synthetic Biology: The Development and Application of MANufacturing on the GO (MANGO)
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Decentralizing Recombinant Protein Biomanufacturing Using Cell-Free Synthetic Biology: The Development and Application of MANufacturing on the GO (MANGO)
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Dissertation
Decentralizing Recombinant Protein Biomanufacturing Using Cell-Free Synthetic Biology: The Development and Application of MANufacturing on the GO (MANGO)
2022
Overview
Recombinant proteins are of extreme importance globally, comprising therapeutics, diagnostics, research tools, and more. Currently, recombinant proteins are synthesized using living hosts in well-resourced laboratories, then distributed globally – often refrigerated – along sometimes fragile supply chains. This model poses a challenge for low-resource and remote communities, and results in a slow-to-respond system when facing outbreaks and emergencies. Cell-free in vitro protein synthesis systems offer a portable small-scale alternative that can be freeze-dried into a room-temperature stable reaction pellet, then rehydrated at the point of need to initiate production of the protein of interest. While cell-free protein synthesis has been used to express proteins in laboratory settings, expansion into the field requires deployment of companion devices to automate the protein synthesis and purification process. This work reports the design, fabrication, and demonstration of MANGO (MANufacturing on the GO), a purpose-built device for portable small-scale protein production.
Publisher
ProQuest Dissertations & Theses
Subject
ISBN
9798357549297
