Journal Article

The protective role of phytochemicals on TiO sub( 2) nanoparticles-induced DNA damage in lymphocytes

Lee, Seong-A, Lee, Mi-Young, Ryu, A-Reum, Bang, In-Chul

2016

Overview

The adverse health effect of nanoparticles is of concern for humans and environment. In the present study, TiO sub( 2)-nanoparticles (TiO sub( 2)-NPs)-induced oxidative DNA damage in lymphocytes was measured by comet assay. 80 mu g ml super( -1) TiO sub( 2)-NPs induced approximately 3-fold increase in DNA damage than in the PBS-control group as measured by olive tail moment. However, on treating vitamin C and N-acetylcysteine, DNA damage was effectively protected in a concentration dependent manner. Moreover, the protective effect of several phytochemicals including berberine, resveratrol, sulforaphane, and curcumin on DNA damage caused by TiO sub( 2)NPs was manifested. The increased olive tail moment induced by TiO sub( 2)-NPs was effectively inhibited by treatment with these phytochemicals. Especially, olive tail moment of 5 mu g ml super( -1) berberine-treated group was significantly reduced down to the level of control group, showing almost complete protection. Taken together, the protective effect of phytochemicals against DNA damage by TiO sub( 2)-NPs may be applied for the development of antidote for TiO sub( 2) toxicity.

• Subject: Olea