Journal Article

A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine

Larson, Tony R., Graham, Ian A., Li, Yi, Kaminski, Filip, Walker, Carol, Bowser, Tim A., He, Zhesi, Teodor, Roxana, Winzer, Thilo, Gazda, Valeria, Meade, Fergus, Kern, Marcelo, Vaistij, Fabián E.

2012

نظرة عامة

Noscapine is an antitumor alkaloid from opium poppy that binds tubulin, arrests metaphase, and induces apoptosis in dividing human cells. Elucidation of the biosynthetic pathway will enable improvement in the commercial production of noscapine and related bioactive molecules. Transcriptomic analysis revealed the exclusive expression of 10 genes encoding five distinct enzyme classes in a high noscapine-producing poppy variety, HN1. Analysis of an F₂ mapping population indicated that these genes are tightly linked in HN1, and bacterial artificial chromosome sequencing confirmed that they exist as a complex gene cluster for plant alkaloids. Virus-induced gene silencing resulted in accumulation of pathway intermediates, allowing gene function to be linked to noscapine synthesis and a novel biosynthetic pathway to be proposed.

• الناشر: American Association for the Advancement of Science,The American Association for the Advancement of Science
• موضوع: Alkaloids / Anticancer properties / antineoplastic agents / Antineoplastic Agents, Phytogenic - biosynthesis / antitussive agents / biochemical pathways / Biological and medical sciences / Biosynthesis / Capsules / Classical genetics, quantitative genetics, hybrids / Clusters / Cough / Enzymes / Flowers & plants / Fundamental and applied biological sciences. Psychology / Genes / Genes, Plant / Genetics of eukaryotes. Biological and molecular evolution / Genomes / heterozygosity / homozygosity / Latex / Libraries / Low level / Metabolites / Molecular Sequence Data / Morphinans / Multigene Family / Narcotics / Noscapine - metabolism / Open reading frames / Papaver - enzymology / Papaver - genetics / Papaver - metabolism / Papaver somniferum / Pharmaceutical sciences / Plant biology / Poppies / Pteridophyta, spermatophyta / Synthesis / Vegetals