Journal Article

Monitoring and Measuring Autophagy

Yoshii, Saori R., Mizushima, Noboru

2017

نظرة عامة

Autophagy is a cytoplasmic degradation system, which is important for starvation adaptation and cellular quality control. Recent advances in understanding autophagy highlight its importance under physiological and pathological conditions. However, methods for monitoring autophagic activity are complicated and the results are sometimes misinterpreted. Here, we review the methods used to identify autophagic structures, and to measure autophagic flux in cultured cells and animals. We will also describe the existing autophagy reporter mice that are useful for autophagy studies and drug testing. Lastly, we will consider the attempts to monitor autophagy in samples derived from humans.

• الناشر: MDPI AG,MDPI
• موضوع: Animals / Autophagosomes - metabolism / Autophagosomes - ultrastructure / Autophagy / Autophagy-Related Proteins - genetics / Autophagy-Related Proteins - metabolism / Humans / Microscopy, Fluorescence - methods / Review