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Integrin-Mediated Extracellular Matrix Effects on Hepatocyte Behavior and the Functionalization of 3D-Printed Polyamide for New Applications
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Integrin-Mediated Extracellular Matrix Effects on Hepatocyte Behavior and the Functionalization of 3D-Printed Polyamide for New Applications
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Integrin-Mediated Extracellular Matrix Effects on Hepatocyte Behavior and the Functionalization of 3D-Printed Polyamide for New Applications
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Dissertation
Integrin-Mediated Extracellular Matrix Effects on Hepatocyte Behavior and the Functionalization of 3D-Printed Polyamide for New Applications
2025
نظرة عامة
With a constantly growing number of drugs being submitted for and denied by FDA review, the development of new methodologies for in vitro analyses has become a critical research topic. The study of drug metabolism and toxicity in liver cells using traditional cell culture substrates, such as culture flasks, is insufficient to capture what occurs in vivo. This phenomenon inspired a variety of techniques for culturing cells in three dimensional matrices that recreate the in vivo physical environment. By electrospinning microfiber scaffolds, cells can be cultured on 3D surfaces and characterized to better understand how a 3D extracellular matrix (ECM) modifies cell behavior. Early research in mechanobiology established that the ECM regulates cell behaviors such as motility, proliferation, and apoptosis. However, there is a lack of research that investigates cellular changes on a metabolic level and how these changes are signaled by the ECM to the cell. The integrins, a family of transmembrane proteins, are known to mechanotransduce information regarding the ECM to the cell. This dissertation addresses how integrins and their conformations are responsible for mediating changes in ECM to changes in cell behavior. Alterations to the ECM can even modify post-translational modifications of integrins to promote certain behaviors such as cell metastasis. Separately, 3D-printing and microfluidics have coevolved for the development of low-volume cell culture systems that mimic in vivo blood flow, nutrient provision, and waste removal. The versatility of 3D printing applications, from tissue engineering to aerospace applications, inspired the development of a chemical functionalization method for improving the hydrophobicity of polyamide materials. In this dissertation the applications of functionalized polyamide for the retention of hydrophobic species are demonstrated for solid-phase extraction, drug-delivery, and enzyme immobilization for the automated detection of glucose.
الناشر
ProQuest Dissertations & Theses
موضوع
ISBN
9798315733539
