Journal Article

Impact of Substrate Amount and Fruiting Induction Methods in Lentinula edodes Cultivation

Hirai, Milton Mineo, Vieira Junior, Wagner Gonçalves, Rocha, Bruno de Souza, Freitas, Marcos Antônio da Silva, Santana, Asser Botelho, de Paula, Adriano Taffarel Camargo, Zied, Diego Cunha, Alves, Lucas da Silva

2025

نظرة عامة

Mushroom production is a sustainable practice but requires improvements, such as in Lentinula edodes (Berk) Pegler cultivation, which has high water and labor demands. In this context, this study proposed replacing the traditional primordia induction method by submersion with a water injection method. Two primordia induction methods (submersion and injection) and two cultivation block formats were compared: rectangular cube (2 kg) and cylindrical (3.5 kg). The substrate, composed of eucalyptus sawdust (72%), wheat bran (12.5%), rice bran (12.5%), CaCO3 (1%), and CaSO4 (2%), was inoculated with strain LED 19/11 and incubated for 80 days at 26 ± 5 °C and 85 ± 15% humidity. After this period, the blocks were washed and transferred to the production environment. Fruiting was induced either by submersion or water injection, and production was evaluated over four harvest flushes. The 2 kg blocks had higher yields with submersion (16.62%), while the 3.5 kg blocks responded better to injection (13.01%), showing more homogeneous production. Increasing the substrate quantity contributes to greater harvest stability across production cycles. Water injections proved to be a viable alternative, reducing handling and facilitating large-scale production. The use of this technique demonstrates great importance in reducing water use and also the need for labor in cultivation.

• الناشر: MDPI AG
• موضوع: Brazil / Calcium carbonate / Cultivation / cultivation methods / Design / Eucalyptus / Fungiculture / High density polyethylenes / Humidity / Injection / Labor / Labor market / Lentinula edodes / Methods / mushroom production / Mushrooms / Plastics / Primordia / Rice / Rice bran / Sawdust / shiitake / Stainless steel / Sustainable practices / Vegetable industry / Water / Water injection / water optimization / Water use / Wheat bran