Journal Article

Flavonoids from Morus alba L. Leaves: Optimization of Extraction by Response Surface Methodology and Comprehensive Evaluation of Their Antioxidant, Antimicrobial, and Inhibition of α-Amylase Activities through Analytical Hierarchy Process

Zou, Xintong, Zhang, Yang, Dong, Yao, Lu, Tenghui, Zhou, Hongli, Cui, Hao, Yang, Xiudong, Wang, Mingxin

2019

نظرة عامة

To explore the flavonoids from L. leaves (MLF), the process of extracting was optimized by a response surface methodology and the antimicrobial and antioxidant activities were evaluated in vitro. The yield of flavonoids reached 50.52 mg g under the optimized extraction conditions (i.e., extraction temperature, 70.85 °C; solvent concentration, 39.30%; extraction time, 120.18 min; and liquid/solid ratio, 34.60:1). The total flavonoids were extracted in organic solvents with various polarities, including petroleum ether (MLF ), ethyl acetate (MLF ), and -butanol (MLF ). In vitro, the four MLF samples exhibited good antioxidant activities for scavenging of 2, 2'-azinobis-(3-ethylbenz-thiazoline-6-sulphonate) radical, 1, 1-diphenyl-2-picrylhydrazyl radical, and total reducing power. Regarding antimicrobial efficacy, the MLF samples suppressed the development of , , and . The MLF samples inhibited α-amylase activity to a certain extent. The analytical hierarchy process (AHP) was used to evaluate comprehensively the bioactivities of the MLF samples. The AHP results revealed that the bioactivity comprehensive score (78.83 μg mL ) of MLF was optimal among the four MLF samples. L. leaves also exhibited non-hemolytic properties. All bioactivities suggested the potential of MLF as a candidate resource in the food and drug industries.

• الناشر: MDPI AG,MDPI
• موضوع: Acetic acid / AHP / alpha-Amylases - antagonists & inhibitors / Amylases / Analytic hierarchy process / Anti-Bacterial Agents - chemistry / Anti-Bacterial Agents - isolation & purification / Anti-Bacterial Agents - pharmacology / Antiinfectives and antibacterials / Antimicrobial agents / Antioxidants / Antioxidants - chemistry / Antioxidants - isolation & purification / Antioxidants - pharmacology / Bacillus - drug effects / Binding sites / bioactivities / Biological activity / Butanol / Design / Enzyme Inhibitors - chemistry / Enzyme Inhibitors - isolation & purification / Enzyme Inhibitors - pharmacology / Ethanol / Ethyl acetate / evaluate comprehensively / Experiments / extraction conditions / Flavonoids / Flavonoids - chemistry / Flavonoids - isolation & purification / Flavonoids - pharmacology / Food industry / Gram-positive bacteria / in vitro / Microbial Sensitivity Tests / Morus - chemistry / Morus alba / mulberry leaves / Optimization / Organic solvents / Petroleum ether / Plant Extracts / Plant Leaves - chemistry / Ratios / Response surface methodology / Scavenging / Solvents / Staphylococcus aureus - drug effects / Temperature / Thiazoline / Variance analysis / α-Amylase