Journal Article

Prevalence, antimicrobial resistance, and genomic characterization of Salmonella strains isolated in Hangzhou, China: a two-year study

Hu, Huangdu, Fu, Ying, Han, Xinhong, Lu, Shanshan, Zhou, Junxin, Mao, Caiping, Yu, Yunsong, Hua, Xiaoting, Yu, Lifei, Jiang, Yan, Fan, Jianzhong

2024

نظرة عامة

This study explored the molecular epidemiology and resistance mechanisms of 271 non-duplicate Salmonella enterica ( S. enterica ) strains, isolated mainly from adults (209/271) in a tertiary hospital in Hangzhou between 2020 and 2021. Through whole-genome sequencing and bioinformatics, the bacterial strains were classified into 46 serotypes and 54 sequence types (ST), with S. Enteritidis, S. 1,4,[5],12:i:-, and S. Typhimurium being the most prevalent serotypes and ST11, ST34, and ST19 the most common STs. The strains isolated from adults were primarily S. Enteritidis (59/209), while from children were mainly S. 1,4,[5],12:i:- (20/62). Worryingly, 12.55% strains were multi-drug resistant (MDR), with resistance rates to cefepime (FEP), ceftazidime (CAZ), ceftriaxone (CRO) and cefotaxime (CTX) of 7.38%, 9.23%, 15.87% and 16.24%, respectively, and resistance rates to levofloxacin (LEV) and ciprofloxacin (CIP) of 8.49% and 19.19%, respectively. It is worth noting that the resistance rates of CRO and CTX in children reached 30.65%. A total of 34 strains carried extended-spectrum β-lactamase (ESBL) genes, dominated by bla CTX-M-65 (13/34) and bla CTX-M-55 (12/34); it is notable that one strain of S . Saintpaul carried both bla CTX-M-27 and bla CTX-M-55 . The resistance mechanism to cephalosporins was mainly due to ESBL genes (20/43), and other genes included AmpC and β-lactamase genes. The strains resistant to quinolones mainly carried qnrS1 (27/53), and others included qnrB6 , aac(6′)-Ib-cr , and mutations in gyrA and parC . One strain did not carry common quinolone resistance genes but had a parC (p.T57S) mutation to cause CIP resistance. This research provides vital insights into the molecular epidemiology and resistance mechanisms of clinical S. enterica , implicating possible infection control strategies.

• الناشر: BioMed Central,BioMed Central Ltd,BMC
• موضوع: Adult / Anti-Bacterial Agents - pharmacology / Antimicrobial resistance / Antimicrobial Resistance and Infection Control / Beta lactamases / beta-Lactamases - genetics / Biochemistry / Biomedical and Life Sciences / Biomedicine / Causes of / Cephaloridine / Cephalosporins / Child / China - epidemiology / Drug Resistance / Drug resistance in microorganisms / Drug Resistance, Multiple, Bacterial - genetics / Drug therapy / Epidemiology / Genetic aspects / Genetic research / Genome, Bacterial / Genomes / Genomics / Health aspects / Humans / Identification and classification / Infectious Diseases / Medical Microbiology / Microbial Genetics and Genomics / Microbial Sensitivity Tests / Molecular Epidemiology / Moxalactam / Parasitology / Prevalence / Risk factors / Salmonella / Salmonella - classification / Salmonella - drug effects / Salmonella - genetics / Salmonella - isolation & purification / Salmonella enterica / Salmonella enterica - classification / Salmonella enterica - drug effects / Salmonella enterica - genetics / Salmonella enterica - isolation & purification / Salmonella food poisoning / Salmonella Infections - epidemiology / Salmonella Infections - microbiology / Serogroup / Statistics / Whole Genome Sequencing