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Glutamate carboxypeptidase II (GCPII) is a membrane-bound metallopeptidase predominantly expressed in neural and prostatic tissues, with significantly elevated levels in prostate carcinoma that increase with tumor grade. Despite its significance as a target for imaging and therapy in prostate cancer, its physiological function in this tissue remains poorly understood. To help fill this knowledge gap, we developed an integrated approach combining proximity labeling technologies for proteomic profiling (horseradish peroxidase, µMap, and riboflavin tetraacetate labeling) with our previously established iBody platform, which targets GCPII with a small-molecule specific inhibitor with proven efficacy as a chemical probe. Proximity labeling proteomic experiments on U251 MG–GCPII cells were followed by mass spectrometry and statistical analysis of protein abundances obtained by label-free quantification. Additionally, selected identified proteins were further validated through Western blot analyses and GCPII pulldown assays using cell lysates. This work identifies a network of GCPII-associated proteins that are potentially involved in cancer metabolism, migration, invasiveness, progression, and immune evasion. Our novel proximity proteomics labeling strategy provides a low-background framework, efficient biotinylation, and enhanced target binding via the avidity effect. Among the approaches tested, riboflavin tetraacetate-based iBody labeling exhibited the highest precision, underscoring its potential for membrane protein interactome mapping.

The main aim of the study was to record subjective assessment of sleep quality between men and woman in hospitalised patients over 3 nights and look for associations with other basic hospitalisation data (age, type of department, surgery, pain, type of admission, previous hospitalisation, sleep-inducing medication). The secondary aim was to determine whether the (FIRST) questionnaire is an appropriate tool for identifying hospitalised individuals prone to situational sleep disturbance. A multicentre descriptive cross-sectional study was conducted in regular surgical and medical departments of seven selected hospitals in the Czech Republic. On the first day of hospitalization, patients completed the FIRST screening questionnaire. Their subjectively perceived sleep quality for the previous night was assessed from the second to the fourth day of hospitalization using the (RCSQ). The study included 340 patients (172 females and 168 males; mean age 58.9 ± 14.9 years and 57.3 ± 15.0 years, respectively). No significant differences in RCSQ scores were observed between men and women or across the three nights of hospitalization. Moderate correlations were identified between hospitalization data and RCSQ scores, with the strongest positive correlation for alprazolam use (Ra = 0.604). Other positive correlations included surgical department hospitalization, sleep-inducing medications, surgery, male sex, and age. The strongest negative correlation was with pain (Ra = -0.498), while other negative correlations included elective admission, medical department hospitalization, and previous hospitalization. The studied factors explained 18% of the RCSQ variability. The association between FIRST scores and RCSQ was statistically significant (p < 0.001) but weak (Spearman's -0.1734, Kendall's tau -0.1234). Subjective sleep quality during hospitalization is related to the type of department, care provided, and pain, age, and sex. There were no significant changes in subjective sleep quality ratings during the first three days of hospitalization. The FIRST questionnaire is not a suitable screening tool for identifying individuals with situational sleep disturbance in hospital.

NOABSTRACTMechanically ventilated patients have disturbed sleep.To explore whether there is a relationship between successful or unsuccessful weaning of patients and their sleep quality and circadian rhythm.A scoping review. The search process involved four online databases: CINAHL, MEDLINE, ProQuest, and ScienceDirect. Original studies published between January 2020 and October 2022 were included in the review.Six studies met the inclusion criteria. These studies showed that patients with difficult weaning were more likely to have atypical sleep, shorter REM sleep, and reduced melatonin metabolite excretion. Muscle weakness was an independent factor associated with prolonged weaning from mechanical ventilation and was significantly more frequent in patients with atypical sleep. Heterogeneous patient samples and the methodology of the studies hamper a clear interpretation of the results.A relationship was found between abnormal sleep patterns, reduced melatonin metabolite (6-sulfa-toxymelatonin) excretion, and unsuccessful weaning. However, the causality is not clear from the existing research.

Aim. To investigate how nurses and patients perceive sleep and point out the factors that negatively impact patients’ sleep during hospitalisation in selected departments in two hospitals in the Czech Republic.Material and methods. An exploratory qualitative study – thematic analysis. We obtained data from three group interviews involving 16 nurses and eight inpatients from selected departments in two hospitals. We conducted an exploratory qualitative study and thematic analysis.Results. The thematic analysis identified four main themes. Theme (1): Importance of sleep. Theme (2): Quality and organisation of nursing care. Theme (3): Experiencing intense emotions and feelings. Theme (4): Health.Conclusions. Hospitalisation often negatively affects patients’ sleep. Patients’ emotions, experiences, and the hospital environment are essential to achieve good sleep. Nurses and the entire health care system have great potential to improve hospitalisation conditions concerning sleep and the emotional mood of patients and thus indirectly influence the general health of patients and their recovery.

Two mouse hybridoma cell lines cultured in different basal media withthe iron-rich protein-free supplement were subjected to deliberatestarvation by inoculation into media diluted with saline to 50% or less.In the diluted media the growth was markedly suppressed and a largefraction of cells died by apoptosis. The cells could be rescued fromapoptotic death by individual additions of amino acids, such as glycine,L-alanine, L-serine, L-threonine, L-proline, L-asparagine, L-glutamine,L-histidine, D-serine, β-alanine or taurine. Amino acids withhydrophobic or charged side chains were without effect. The apoptosispreventing activity manifested itself even in extremely diluted media,down to 10% of the standard medium. The activity of L-alanine in theprotection of cells starving in 20% medium was shown also in semicontinuousculture. In the presence of 2 mM L-alanine the steady-state viable cell density more than doubled, with respect to control, andthe apoptotic index dropped from 37% in the control to 16%. It wasconcluded that the apoptosis-preventing amino acids acted as signalmolecules, rather than nutrients, and that the signal had a character ofa survival factor. The specificity of present results, obtained with twodifferent hybridomas, supports our view (Franěk and Chládková-Šrámková, 1995) that the membranetransport macromolecules themselves may play the role of therecognition elements in a signal transduction pathway controlling thesurvival of hybridoma cells.