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S792.95; 以幼苗茎段为外植体建立了生长黄酮的悬浮细胞培养体系,对细胞生长周期、相应的黄酮积累量变化以及苯丙氨酸解氨酶(PAL)活性进行了研究.结果表明,黄酮的积累在细胞生长对数期内增加,于15 d达最高峰.在黄酮积累的过程中PAL活性也呈增加趋势.当培养细胞转换新鲜培养基时,PAL活性增加了2倍(从0.05到0.15OD).用紫外线处理,培养于暗中细胞的PAL活性增加了11倍(从0.05到0.60OD).

The cell suspension culture system was established for producing flavoniods by using stem of young seedling as ex-plants. A study was made on the phase of cell growth and relative changing amount of flavonoids, as well as the activity of L-phenylalanine ammonia-lyase (PAL) (EC 4. 3.1.5). It was showed that the flavonoids accumulation increased in the exponential phase of cell growth, and reached the highest on the 15th day after culture. The activity of PAL increased during this period. PAL activity doubled in response to transferring suspension-cultured cells into new medium. In cell suspension cultures, growth in the dark, but not in light-growth cultures, PAL activity was induced up to 12 times by UV-light. 以幼苗茎段为外植体建立了生长黄酮的悬浮细胞培养体系,对细胞生长周期、相应的黄酮积累量变化以及苯丙氨酸解氨酶(PAL)活性进行了研究。结果表明,黄酮的积累在细胞生长对数期内增加,于15 d达最高峰。在黄酮积累的过程中PAL活性也呈增加趋势。当培养细胞转换新鲜培养基时,PAL活性增加了2倍(从0．05到0．15 OD)。用紫外线处理,培养于暗中细胞的PAL活性增加了11倍(从0．05到0．60 OD)。