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2 result(s) for "韩蕾 孙振元 郭燕 佟一超 李银凤 彭镇华"
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空间环境对草地早熟禾叶片解剖结构及同工酶酶谱的影响
将“神舟”三号(SZ-3)飞船搭载的草地早熟禾品种纳苏干种子地面种植后,根据形态变化进行初步筛选,并通过分株扩繁出突变株系PM1、PM2和PM3。对这三个草地早熟禾突变株系的叶片解剖结构及过氧化物酶同工酶和酯酶同工酶进行观察。结果表明,与对照(CK)相比,三个变异株系由于细胞数目的增加使叶片宽度明显增加;叶肉细胞平均直径降低使叶片厚度减少;PM2、PM3株系叶片泡状细胞的增多,改变了叶片的形态。过氧化物酶同工酶谱带CK有11条,迁移率为4.63%~47.22%;PM1有21条谱带,迁移率为2.78%~68.52%;PM2有18条谱带,迁移率为2.78%~67.59%;PM3有20条谱带,迁移率为2.78%~63.89%。酯酶同工酶谱带CK有3条,迁移率为59.74%~94.81%;PM1有8条谱带,迁移率为38.96%~94.81%;.PM2有7条谱带,迁移率为48.05%~94.81%;PM3有8条谱带,迁移率为49.35%~94.81%。PM1、PM2和PM3三个株系与CK含有一部分完全相同的谱带,另一部分谱带发生了较大变化:分别出现了CK中没有的谱带和在CK中存在而三个变异株系没有的谱带。同时各谱带的强弱也发生了不同程度的变化。
The Effect of the Space Condition on the Anatomical Characters of the Leaves and the Ioszyme of Peroxidase and Esterase in Poa pratensis / 空间环境对草地早熟禾叶片解剖结构及同工酶酶谱的影响
Taking the dry seeds of Poa pratensis Nassau carried by SZ-3 as the treatment and the seeds which on the earth as CK,three mutations were screened based on presentational characters from the plants treatment and asexual reproduction as PM1,PM2 and PM3.The effect of the space condition on the anatomical characters of the leaves and the ioszyme of peroxidase and esterase in leaves were observed and investigated.The results showed that compared to CK,the width of the leaves of the three mutations increased as the number of epidermis increased,the thickness of the leaves reduced as the average diameter of the mesophyllous cells decreased.The number of the bulliform cells in the leaves of PM2 and PM3 increased which changed the shape of the leaves.The results of the ioszyme of esterase showed that there were 11 bands in CK and the Rf value was 4.63%~47.22%;21 bands in PM1 and Rf value was 2.78%~68.52%;18 bands in PM2 and Rf value was 2.78%~67.59%;20 bands in PM3 and Rf value was 2.78%~63.89%.The results of the ios