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Adulteration and mislabeling have become a very common global malpractice in food industry. Especially foods of animal origin are prepared from plant sources and intentionally mislabeled. This type of mislabeling is an important concern in food safety as the replaced ingredients may cause a food allergy or toxicity to vulnerable consumers. Moreover, foodborne pathogens also pose a major threat to food safety. There is a dire need to develop strong analytical tools to deal with related issues. In this context, proteomics stands out as a promising tool used to report the aforementioned issues. The development in the field of omics has inimitable advantages in enabling the understanding of various biological fields especially in the discipline of food science. In this review, current applications and the role of proteomics in food authenticity, safety, and quality and food traceability are highlighted comprehensively. Additionally, the other components of proteomics have also been comprehensively described. Furthermore, this review will be helpful in the provision of new intuition into the use of proteomics in food analysis. Moreover, the pathogens in food can also be identified based on differences in their protein profiling. Conclusively, proteomics, an indicator of food properties, its origin, the processes applied to food, and its composition are also the limelight of this article. In this context, proteomics stands out as a promising tool used to report aforementioned issues. The development in the field of omics has inimitable advantages in enabling the understanding of various biological fields especially in the discipline of food science. In this review, current applications and role of proteomics in food authenticity, safety, and quality and food traceability are highlighted comprehensively. Additionally, the other components of proteomics have also been comprehensively described.

Nanotechnology has revolutionized the field of food systems, diagnostics, therapeutics, pharmaceuticals, the agriculture sector, and nutraceuticals. Nanoparticles are playing important role in giving the solution to enhance bioavailability of oral delivery of bioactive compounds. This review revealed that nanoparticles can improve the bioavailability of micronutrients, for example, vitamin B12, vitamin A, folic acid, and iron. However, toxicity associated with nanoparticle‐based delivery systems is still a major concern after ingestion of nano‐based supplements. The mode of the mechanism of nanomaterial along with bioactive components in different physiological conditions of the human body is also a major gap in the field of nanoceuticals. In the future, more evidence‐based clinical investigations are needed to confirm the exact approach to physiological changes in the human body. This review revealed that nanoparticles can improve the bioavailability of micronutrients, for example, vitamin B12, vitamin A, folic acid, and iron. However, toxicity associated with nanoparticle‐based delivery systems is still a major concern.

The target of this research was to determine sugar profile, volatile compounds, minerals content and antioxidant properties of the Sukkari date flesh as methanolic and ethanolic extracts. Sukkari date showed sugar 78.32% (dry weight), while fibre, crude protein, ash and crude fat were 3.15, 3.01, 2.30 and 0.56, respectively. Glucose (51.80%), fructose (47.50%), while small amount sucrose, fucose and galacturonic acid were also detected. Potassium, calcium and magnesium were observed to be the predominant. Twenty-two components were identified; 5-Hydroxymethylfurfural was present in the highest amount (27.25%), followed by 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (9.45%). Total phenolic and flavonoid content of methanolic extract were 62.50 mg GAE/100 g and 3.20 mg CE/100 g, respectively, against 60.25 mg GAE/100 g and 2.90 mg CE/100 g respectively, for ethanolic extracts. Three assays including DPPH, reducing power and ABTS radical scavenging activities showed a good antioxidant activity of date palm extract. Sukkari date was observed to have good nutritional and antioxidant characteristics and can be utilize as potential nutrition.

This research aimed to evaluate the influences of the pulsed electric field (PEF), ultrasound (US), and combination between them (PEF + US) on the quality of vinegar processed from date palm fruits compared with untreated vinegar (UT). Physicochemical properties, free amino acids (FAA), volatile components, organic acids, total phenolics and flavonoids, and sensory analysis were determined. The results showed that there were no significant differences in pH, total titratable acidity, ethanol content, and total sugar in all treated vinegar compared with UT. However, the values were found to be decreased (PEF + US < PEF < US < UT). Twenty-eight compounds were identified in the vinegar treated by PEF + US as the highest number of components, followed by PEF and US (23 and 22 components, respectively), compared with 19 compounds identified in UT. Compared with UT, there was a significant increase (p < 0.05) in the total FAA in dates vinegar among all treated samples (UT < US < PEF < PEF + US). Total phenolic and flavonoids contents results indicated that there was a significant increase (p < 0.05) in the treated vinegar compared with UT. Sensory analysis results indicated that no significant difference (p < 0.05) in all the parameters, except for a quite significant difference (p < 0.05) in the overall acceptability between the treated vinegar. In this study, vinegar was successfully produced from date palm fruits. Therefore, PEF + US are capable not only in enhancing the extraction process but also in the production of vinegar with good quality.

The current study was aimed to observe the influence of pulsed electric field (PEF) on the extraction of bioactive components; antioxidant activity and physicochemical properties of date palm fruit extract (DPFE) as compared to the extract untreated by PEF. The fruit was treated with PEF (frequency: 10 Hz, time: 100 µs, pulses number: 30, electric field strength (EFS): 1, 2, and 3 kV/cm. The results show that PEF has a positive impact on the total content of carotenoids, anthocyanins, flavonoids, and phenolics by increasing the EFS. DPFE treated with PEF exhibit a strong antioxidant activity as compared to untreated extract, while electrical conductivity, pH values, and titratable acidity were not affected by PEF. The results indicate a notable increase in the volatile components in DPFE treated with PEF at 3 kV/cm. Thus, PEF treatment can assist the ethanolic extraction of DPFE to improve the bioactivity and antioxidative activity. These findings suggest that PEF can be a more suitable technique to enhance solvent extraction on a commercial basis.

This study investigates the ecological interaction between honeybees ( Apis mellifera ) and fennel ( Foeniculum vulgare ) plants, examining the mutual benefits of this relationship. Field experiments conducted in Egypt from December 2022 to May 2023 recorded diverse insect pollinators attracted to fennel flowers, especially honeybees. Assessing honeybee colonies near fennel fields showed improvements in sealed brood (357.5–772.5 cells), unsealed brood (176.3–343.8 cells), pollen collection (53.25–257.5 units), honey accumulation (257.5–877.5 units), and colony strength (7.75–10) over three weeks. Fennel exposure explained 88–99% of variability in foraging metrics. Comparing open versus self-pollinated fennel revealed enhanced attributes with bee pollination, including higher flower age (25.67 vs 19.67 days), more seeds per umbel (121.3 vs 95.33), bigger seeds (6.533 vs 4.400 mm), heavier seeds (0.510 vs 0.237 g/100 seeds), and increased fruit weight per umbel (0.619 vs 0.226 g). Natural variation in seed color and shape also occurred. The outcomes demonstrate the integral role of honeybees in fennel agroecosystems through efficient pollination services that improve crop productivity and quality. Fennel provides abundant nutritional resources that bolster honeybee colony health. This research elucidates the symbiotic bee-fennel relationship, underscoring mutualistic benefits and the importance of ecological conservation for sustainable agriculture.

To discover more innovative, high-nutraceutical foods that can be used in other food production systems, this research was carried out to investigate the sensory properties and physical-chemical of functional bread by enhancing alhydwan. Alhydwan contains a high amount of dietary fiber (36.13%), protein (14.60%), ash (6.88%), and fat (11.49%) as well as substantial amounts of unsaturated fatty acids; 74.63% the fatty acids. We assessed the impact of supplanting of 5,10 and 15% of wheat flour by alhydwan flour on bread dough rheology and bread quality. With increased levels of alhydwan, a positive increase in dough stickiness and water absorption was observed. The softness of the crumb increased with the alhydwan level. At the alhydwan supplement of 10 g/100 g, the highest sensory assessment score was recorded. The microstructure of alhydwan enhanced formulation showed the starch granules more advanced degrees of gelatinization. FTER examination of alhydwan revealed remarkable differences alhydwan flour were stronger than those in the control. Our studies showed that alhydwan can be integrated into bread to enhance the quality of bread and dough rheology, which can be used to enhance functional properties of bread, moreover, addition of alhydwan to the cereal flour may affect the characteristics of processed food and can help to maintain the smooth texture of the resultant products.

Aluminum (Al) is an important factor in the environment as it is used in agriculture and several industries leading to hazardous effects via oxidative stress. Bromelain is a cheap extract from the byproduct waste of Ananas comosus stem. It has been used in several biological and therapeutic applications. So, this study was undertaken to assess the hepatoprotective potential of bromelain versus oxidative stress induced by aluminum chloride in rats. Results revealed that administration of AlCl3 reduced the body and liver weights and increased Al concentration in the blood and liver tissue. Also, AlCl3 caused valuable changes in hematological parameters and increased TBARS and H2O2 concentrations in rat liver. Enzymatic (SOD, CAT, GPx, GR, and GST) and nonenzymatic (GSH) antioxidants and protein content were significantly decreased. Furthermore, alterations in liver biomarkers such as bilirubin level and enzyme activities in both serum and liver homogenate (LDH, ALP, AST, and ALT) were detected. AlCl3 also caused inflammation as indicated by upregulation of the inflammation-related genes [interleukin 1 beta (IL-1β)], tumor necrosis factor-alpha (TNF-α), as well as matrix metalloproteinase (MMP9), and downregulation of nuclear factor erythroid 2 (Nrf2) expression. In addition, histopathological examination showed significant variations in the liver that confirms the biochemical results. Otherwise, bromelain intake alone slumped lipid peroxidation and gotten better antioxidant status significantly. Moreover, supplementation with bromelain before AlCl3 intoxication restores enzymatic and nonenzymatic antioxidants as well as biochemical indices and tissue architecture with respect to the AlCl3 group. In conclusion, bromelain proved its remarkable protective power to abolish AlCl3 toxicity. So, it might represent a new strategy in the therapy of metal toxicity by its antioxidant capacity.

Assessing the embryotoxicity and teratogenicity of various substances and processes is crucial due to their complexity and resource intensity. The chicken embryo (CE) serves an ideal model for simulating the first months of mammalian embryonic development. This makes the CE a reliable model for testing teratogenic effects, particularly in relation to the nervous system (NS), which experiences developmental abnormalities second in frequency only to cardiovascular teratogenic disorders. Microcomputed tomography (μCT) is a promising method for studying these processes. The advantages of μCT include relatively high research speed, diagnostic accuracy, high resolution and the ability to visualize the entire internal 3D structure of an object while preserving for other types of research. At the same time, there are practically no available databases of normative μCT data, both qualitative and quantitative, which would act as a starting point for screening detection of abnormalities in the development of the NS. In this study, we present a simple method for obtaining very detailed quantitative sets of 2D and 3D μCT data of NS structures of the CE ( Gallus Gallus domesticus ) at HH22-HH41 embryonic stages with contrasting by 1% phosphotungstic acid. The results of μCT demonstrate the exact boundaries, high general and differentiated contrast of the main and specific structures of the NS of CE, which are quantitatively and qualitatively similar to results of histological analysis. Calculations of the X-ray density and volume of the main structures of the NS at constant exponential growth are presented. In addition to the increase in linear dimensions, significant changes in the structures of various parts of the brain were identified and visualized during the CE development at HH22 to HH41 embryonic stages. The data presented establish the first methodology for obtaining normative data, including subtle localized differences in the NS in CE embryogenesis. The data obtained open up new opportunities for modern embryology, teratology, pharmacology and toxicology.

The synergetic enhancement effect of the polyaniline (PANI) integration process on the adsorption properties of the PANI/zeolite-A composite (PANI/ZA) as an adsorbent for malachite green and Congo red synthetic dyes was evaluated based on classic equilibrium modelling in addition to the steric and energetic parameters of advanced isotherm studies. The PANI/ZA composite displays enhanced adsorption capacities for both methylene blue (270.9 mg/g) and Congo red (235.5 mg/g) as compared to ZA particles (methylene blue (179.6 mg/g) and Congo red (140.3 mg/g)). The reported enhancement was illustrated based on the steric parameters of active site density (Nm) and the number of adsorbed dyes per active site (n). The integration of PANI strongly induced the quantities of the existing active sites that have enhanced affinities towards both methylene blue (109.2 mg/g) and Congo red (92.9 mg/g) as compared to the present sites on the surface of ZA. Every site on the surface of PANI/ZA can adsorb about four methylene blue molecules and five Congo red molecules, signifying the vertical orientation of their adsorbed ions and their uptake by multi-molecular mechanisms. The energetic investigation of the methylene blue (−10.26 to −16.8 kJ/mol) and Congo red (−9.38 to −16.49 kJ/mol) adsorption reactions by PANI/ZA suggested the operation of physical mechanisms during their uptake by PANI/ZA. These mechanisms might involve van der Waals forces, dipole bonding forces, and hydrogen bonding (<30 kJ/mol). The evaluated thermodynamic functions, including enthalpy, internal energy, and entropy, validate the exothermic and spontaneous behaviours of the methylene blue and Congo red uptake processes by PANI/ZA.