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Hydropower provides continuous and clean energy for human consumption but also brings a series of environmental concerns to local watersheds. Gas bubble disease or mass mortality in fish can be attributed to total dissolved gas (TDG) supersaturation, which occurs when water is released from dams. It is possible to create temporary refuges for fish suffering from supersaturated total dissolved gas (STDG) by strategically arranging aeration facilities along rivers or reservoirs and using the bubbles generated by aeration to increase the dissipation of STDG. The critical limitation to the widespread application of this approach in engineering is the insufficient understanding of the mass transfer mechanisms of STDG under aerated conditions and the transport characteristics of STDG in water flows. In this work, the mass transfer (MT) mechanisms of STDG under aerated conditions were systematically studied via experiments, image processing, and numerical simulation. An innovative three‐dimensional numerical model was established to forecast the MT process of STDG under aerated conditions. The determination of STDG MT in the model incorporated a sophisticated approach that accounted for the dynamic changes in bubble sizes resulting from diverse mechanisms of bubble coalescence and breakup. To validate and calibrate the model, precise aeration experiments were executed at various aeration intensities to gather data on the bubble size distribution, total gas holdup, and STDG dissipation rates. Furthermore, a numerical model was used to quantitatively investigate the impact of the aerator installation depth on STDG dissipation performance. The results revealed that the relationship between the dissipation coefficients of STDG and the aerator installation depth followed a power function. This research can enhance the understanding of the MT characteristics of STDG under aeration conditions while also providing a useful tool for studying the design and optimization of facilities related to STDG engineering treatment via aeration measures. Plain Language Summary The issue of supersaturated total dissolved gas (STDG) can arise downstream of dams, leading to fish bubble disease and potentially death. As the number of dams increases, the STDG problem has emerged as a significant threat to aquatic life. Strategically placing aeration facilities along rivers or reservoirs and utilizing the bubbles they generate can provide temporary refuges for fish affected by STDG, aiding in its dissipation. Aeration can mitigate the negative effects of STDG on fish. A numerical model is developed to predict the STDG mass transfer process in an aerated flow, considering the dynamics of bubble aggregation and fragmentation. The model was calibrated via a precise aeration experiment and also used to determine the quantifiable relationship between STDG dissipation rates and the depth of aerator installation configurations. This research can enhance our comprehension of the mass transfer mechanisms of STDG under aerated flow and also furnish a tool for the design and optimization of engineering facilities.

Hyperthermophilic Thermotoga spp. are excellent candidates for the biosynthesis of cellulosic ethanol producing strains because they can grow optimally at 80 °C with ability to degrade and utilize cellulosic biomass. In T. neapolitana ( Tne ), a putative iron-containing alcohol dehydrogenase was, for the first time, revealed to be a bifunctional aldehyde/alcohol dehydrogenase (Fe-AAdh) that catalyzed both reactions from acetyl-coenzyme A (ac-CoA) to acetaldehyde (ac-ald), and from ac-ald to ethanol, while the putative aldehyde dehydrogenase (Aldh) exhibited only CoA-independent activity that oxidizes ac-ald to acetic acid. The biochemical properties of Fe-AAdh were characterized, and bioinformatics were analyzed. Fe-AAdh exhibited the highest activities for the reductions of ac-CoA and acetaldehyde at 80–85 °C, pH 7.54, and had a 1-h half-life at about 92 °C. The Fe-AAdh gene is highly conserved in Thermotoga spp., Pyrococcus furiosus and Thermococcus kodakarensis , indicating the existence of a fermentation pathway from ac-CoA to ethanol via acetaldehyde as the intermediate in hyperthermophiles.

The quality and safety of food is an increasing concern for worldwide business. Non-destructive methods (NDM), as a means of assessment and instrumentation have created an esteemed value in sciences, especially in food industries. Currently, NDM are useful because they allow the simultaneous measurement of chemical and physical data from food without destruction of the substance. Additionally, NDM can obtain both quantitative and qualitative data at the same time without separate analyses. Recently, many studies on non-destructive detection measurements of agro-food products and final quality assessment of foods were reported. As a general statement, the future of using NDM for assessing the quality of food and agricultural products is bright; and it is possible to come up with interesting findings through development of more efficient and precise imaging systems like the machine vision technique. The present review aims to discuss the application of different non-destructive methods (NDM) for food quality and safety evaluation.

Geopolymer concrete (GPC) has drawn widespread attention as a universally accepted ideal green material to improve environmental conditions in recent years. The present study systematically quantifies and compares the environmental impact of fly ash GPC and ordinary Portland cement (OPC) concrete under different strength grades by conducting life cycle assessment (LCA). The alkali activator solution to fly ash ratio (S/F), sodium hydroxide concentration (CNaOH), and sodium silicate to sodium hydroxide ratio (SS/SH) were further used as three key parameters to consider their sensitivity to strength and CO2 emissions. The correlation and influence rules were analyzed by Multivariate Analysis of Variance (MANOVA) and Gray Relational Analysis (GRA). The results indicated that the CO2 emission of GPC can be reduced by 62.73%, and the correlation between CO2 emission and compressive strength is not significant for GPC. The degree of influence of the three factors on the compressive strength is CNaOH (66.5%) > SS/SH (20.7%) > S/F (9%) and on CO2 emissions is S/F (87.2%) > SS/SH (10.3%) > CNaOH (2.4%). Fly ash GPC effectively controls the environmental deterioration without compromising its compressive strength; in fact, it even in favor.

Analysis of crop genetic diversity and structure provides valuable information needed to broaden the narrow genetic base as well as to enhance the breeding and conservation strategies of crops. In this study, 95 Austrian and Belgian wheat cultivars maintained at the Centre for Genetic Resources (CGN) in the Netherlands were characterised using 1052 diversity array technology (DArT) markers to evaluate their genetic diversity, relationships and population structure. The rarefacted allelic richness recorded in the Austrian and Belgian breeding pools (A25 = 1.396 and 1.341, respectively) indicated that the Austrian germplasm contained a higher genetic diversity than the Belgian pool. The expected heterozygosity (HE) values of the Austrian and Belgian pools were 0.411 and 0.375, respectively. Moreover, the values of the polymorphic information content (PIC) of the Austrian and Belgian pools were 0.337 and 0.298, respectively. Neighbour-joining tree divided each of the Austrian and Belgian germplasm pools into two genetically distinct groups. The structure analyses of the Austrian and Belgian pools were in a complete concordance with their neighbour-joining trees. Furthermore, the 95 cultivars were compared to 618 wheat genotypes from nine European countries based on a total of 141 common DArT markers in order to place the Austrian and Belgian wheat germplasm in a wider European context. The rarefacted allelic richness (A10) varied from 1.224 (Denmark) to 1.397 (Austria). Cluster and principal coordinates (PCoA) analyses divided the wheat genotypes of the nine European countries into two main clusters. The first cluster comprised the Northern and Western European wheat genotypes, whereas the second included the Central European cultivars. The structure analysis of the 618 European wheat genotypes was in a complete concordance with the results of cluster and PCoA analyses. Interestingly, a highly significant difference was recorded between regions (26.53%). In conclusion, this is the first study to reveal the high diversity levels and structure of the uncharacterised Austrian and Belgian wheat germplasm maintained at the CGN as well as place them in a wider European context. The results should help plant breeders to utilise the most promising wheat genotypes of this study in future breeding programmes for enhancing wheat cultivars.

The present study evaluated the sequential biodiesel-bioethanol production from seaweeds. A total of 22 macroalgal species were collected at different seasons and screened based on lipid and carbohydrate contents as well as biomass production. The promising species was selected, based on the relative increase in energy compounds (REEC, %), for further energy conversion. Seasonal and annual biomass yields of the studied species showed significant variations. The rhodophyte Amphiroa compressa and the chlorophyte Ulva intestinalis showed the highest annual biomass yield of 75.2 and 61.5 g m −2 year −1 , respectively. However, the highest annual carbohydrate productivity (ACP) and annual lipid productivity (ALP) were recorded for Ulva fasciata and Ulva intestinalis (17.0 and 3.0 g m −2 year −1 , respectively). The later was selected for further studies because it showed 14.8% higher REEC value than Ulva fasciata. Saturated fatty acids (SAFs) showed 73.4%, with palmitic acid as a dominant fatty acid (43.8%). Therefore, biodiesel showed high saturation degree, with average degree of unsaturation (ADU) of 0.508. All the measured biodiesel characteristics complied the international standards. The first route of biodiesel production (R1) from Ulva intestinalis showed biodiesel recovery of 32.3 mg g −1 dw. The hydrolysate obtained after saccharification of the whole biomass (R2) and lipid-free biomass (R3) contained 1.22 and 1.15 g L −1 , respectively, reducing sugars. However, bioethanol yield from R3 was 0.081 g g −1 dw, which represented 14.1% higher than that of R2. Therefore, application of sequential biofuel production using R3 resulted in gross energy output of 3.44 GJ ton −1 dw, which was 170.9% and 82.0% higher than R1 and R2, respectively. The present study recommended the naturally-grown Ulva intestinalis as a potential feedstock for enhanced energy recovery through sequential biodiesel-bioethanol production.

The isolated microalga Chlorella sorokiniana BENHA721_ABO4 was grown in Bold’s basal medium (BBM) as a control, municipal wastewater (WW), and wastewater enriched with BBM elements (WW+). Cultivation in WW+ showed the highest cell number which represented 25.3 and 47.3% over that grown in WW and BBM, respectively. However, rapid growth in WW+ was accompanied by significant reduction in lipid content. Due to lipid accumulation in WW, it showed the maximum significant lipid productivity of 16.2 mg L−1 day−1. Microalgae cultivation in WW for 10 days showed 74.2, 83.3, and 78.0% removal efficiency for NO3–N, NH3–N and TP, respectively. In addition, growth in WW significantly reduced polyunsaturated fatty acids by 36.0% with respect to BBM in favor of monounsaturated fatty acids. The present results confirmed that C. sorokiniana isolate BENHA721_ABO4 grown in secondary effluent municipal wastewater offers real potential for future application in wastewater treatment and biodiesel production.

Arthrospira platensis is one of the most important cultured microalgal species in the world. Arthrospira complete dry biomass (ACDB) has been reported as an interesting feedstock for many industries, including biodiesel production. The A. platensis by-product of biodiesel production (lipid-free biomass; LFB) is a source of proteins, functional molecules, and carbohydrates, and can also be reused in several applications. The current study investigated the efficiency of ACDB and LFB in bioremediation of dye (Ismate violet 2R, IV2R) from textile effluents. In addition, the potential of ACDB and LFB loaded by IV2R as a feed for Rotifer, Brachionus plicatilis, was examined. The surface of the adsorbents was characterized by SEM, FTIR, and Raman analysis to understand the adsorption mechanism. The batch sorption method was examined as a function of adsorbent dose (0.02–0.01 g L−1), solution initial concentration (10–100 mg L−1), pH (2–10), and contact time (15–180 min). The kinetic studies and adsorption isotherm models (Freundlich, Langmuir, Tempkin, and Halsey) were used to describe the interaction between dye and adsorbents. The results concluded that the adsorption process increased with increasing ACDB and LFB dose, contact time (120 min), initial IV2R concentration (10 mg L−1), and acidity pH (2 and 6, respectively). For the elimination of industrial textile wastewater, the ACDB and LFB sorbents have good elimination ability of a dye solution by 75.7% and 61.11%, respectively. The kinetic interaction between dye and adsorbents fitted well to Langmuir, Freundlish, and Halsey models for LFB, and Langmuir for ACDB at optimum conditions with R2 > 0.9. In addition, based on the bioassay study, the ACDB and LFB loaded by IV2R up to 0.02 g L−1 may be used as feed for the marine Rotifer B. plicatilis.

Microalgae are considered as a promising feedstock for biomass production. The selection of the most suitable species is based on several key parameters such as lipid and fatty acid productivity. In the present study, the growth of different microalgae strains was examined in freshwater media for photoautotrophs suited for large-scale applications to identify the most suitable medium for each species. In the optimal medium, Scenedesmus obliquus showed the highest biomass productivity measured as increase of cell dry weight (0.25 g cellu dry weight (CDW) L⁻¹ day⁻¹), while Botryococcus braunii showed the highest lipid and total fatty acid content (430 and 270 mg g⁻¹ CDW, respectively) among the tested species. Regarding lipid and total fatty acid productivity, S. obliquus was the most lipid and total fatty acid productive strain with 41 and 18 mg L⁻¹ day⁻¹ during the exponential phase, respectively. Additionally, the proportion of saturated and monounsaturated fatty acids increased with duration of the incubation in S. obliquus, while polyunsaturated fatty acids decreased. These results nominate S. obliquus as a promising microalga in order to serve as a feedstock for renewable energy production.

Cost-effective biomass drying is a key challenge for energy recovery from biomass by direct combustion, gasification, and pyrolysis. The aim of the present study was to optimize the process of biomass drying using hot air convection (HA), infrared (IR), and combined drying systems (IR-HA). The specific energy consumption (SEC) decreased significantly by increasing the drying temperature using convective drying, but higher air velocities increased the SEC. Similarly, increasing air velocity in the infrared dryer resulted in a significant increase in SEC. The lowest SEC was recorded at 7.8 MJ/kg at an air velocity of 0.5 m/s and an IR intensity of 0.30 W/cm2, while a maximum SEC (20.7 MJ/kg) was observed at 1.0 m/s and 0.15 W/cm2. However, a significant reduction in the SEC was noticed in the combined drying system. A minimum SEC of 3.8 MJ/kg was recorded using the combined infrared-hot air convection (IR-HA) drying system, which was 91.7% and 51.7% lower than convective and IR dryers, respectively. The present study suggested a combination of IR and hot air convection at 60 °C, 0.3 W/cm2 and 0.5 m/s as optimum conditions for efficient drying of biomass with a high water content.