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Understanding water stress signaling mechanisms and screening for tolerant cocoa cultivars are major challenges when facing prolonged dry and rainy seasons in cocoa-producing areas. While abscisic acid (ABA) and proline are supposed to enhance drought tolerance in cocoa, the role of polyamines remains unclear. The aim of this study was to investigate the biochemical response and phenological adaptation of cocoa (Theobroma cacao) on different soil moisture conditions, with a focus on short-term (20 days) and long-term (89 days) stress conditions, and to compare the performance of three cocoa cultivars. In a split plot design with four blocks, cocoa seedlings of an international high-yielding cultivar (TSH-565) and two locally selected cultivars (IIa-22 and III-06) from the drought-exposed Alto Beni region, Bolivia, were arranged in pots under a roof shelter (cultivar: three levels). The seedlings were exposed to strong (VERY DRY) and moderate (DRY) soil moisture deficits, water logging (WET) and regular irrigation (MOIST) that served as a control (moisture: four levels). We examined the growth performance and the levels of ABA, proline, and polyamines in the leaves. Growth was reduced already at a moderate drought, while severe drought enhanced seedling mortality. Severe drought increased the levels of ABA by 453% and of proline by 935%, inducing a short-term stress response; both compounds were degraded over the long-term period. The polyamine concentration was unrelated to soil moisture. The cocoa cultivars did not differ in their biochemical response to soil moisture stress (proline: p-value = 0.5, ABA: p-value = 0.3), but the local cultivar III-06 showed a stronger height growth increment than the international cultivar TSH-565 (237%, p-value = 0.002) under drought conditions.

Management practices such as tillage, crop rotation, irrigation, organic and inorganic inputs application are known to influence diversity and function of soil microbial populations. In this study, we investigated the effect of conventional versus organic farming systems at low and high input levels on structure and diversity of prokaryotic microbial communities. Soil samples were collected from the ongoing long-term farming system comparison trials established in 2007 at Chuka and Thika in Kenya. Physicochemical parameters for each sample were analyzed. Total DNA and RNA amplicons of variable region (V4-V7) of the 16S rRNA gene were generated on an Illumina platform using the manufacturer's instructions. Diversity indices and statistical analysis were done using QIIME2 and R packages, respectively. A total of 29,778,886 high quality reads were obtained and assigned to 16,176 OTUs at 97% genetic distance across both 16S rDNA and 16S rRNA cDNA datasets. The results pointed out a histrionic difference in OTUs based on 16S rDNA and 16S rRNA cDNA. Precisely, while 16S rDNA clustered by site, 16S rRNA cDNA clustered by farming systems. In both sites and systems, dominant phylotypes were affiliated to phylum Actinobacteria, Proteobacteria and Acidobacteria. Conventional farming systems showed a higher species richness and diversity compared to organic farming systems, whilst 16S rRNA cDNA datasets were similar. Physiochemical factors were associated differently depending on rRNA and rDNA. Soil pH, electrical conductivity, organic carbon, nitrogen, potassium, aluminium, zinc, iron, boron and micro-aggregates showed a significant influence on the observed microbial diversity. The observed higher species diversity in the conventional farming systems can be attributed to the integration of synthetic and organic agricultural inputs. These results show that the type of inputs used in a farming system not only affect the soil chemistry but also the microbial population dynamics and eventually the functional roles of these microbes.

The growing need for accessible alternatives to synthetic insecticides has renewed interest in farmer-made botanical formulations for pest management. This study evaluated the efficacy of selected homemade botanical insecticides against diamondback moth ( Plutella xylostella ) and cabbage aphid ( Brevicoryne brassicae ) on cabbage ( Brassica oleracea var. capitata ) and kale ( B. oleracea var. acephala ) under laboratory and semi-field conditions. Three homemade formulations were assessed: a one-day-old multi-plant extract with neem ( Azadirachta indica ; PE + N), a one-day-old extract without neem (PE), and a fourteen-day-old, fermented extract without neem (PE14). These were compared with three commercial botanical products. In replicated leaf- and plant-based bioassays, neem-based treatments (PE + N and Nimbecidine) consistently caused higher pest mortality than non-neem formulations. Both treatments achieved over 50% mortality of P. xylostella and B. brassicae at higher concentrations, with shorter median lethal times (LT 50 ) and lower median lethal concentrations (LC 50 ). Formulations without neem remained below the 50% mortality threshold under most conditions. Efficacy was lower under semi-field conditions than in laboratory assays, reflecting environmental influences on botanical stability. These findings demonstrate that neem-based homemade botanicals derived from farmer practices can provide pest suppression comparable to commercial biopesticides under controlled and semi-field conditions. While variability in efficacy and lack of formulation standardization remain challenges, such botanicals represent a promising complementary option within integrated pest management strategies for smallholder farming systems.

Thermophilic composting is a promising soil and waste management approach involving diverse micro and macro-organisms, including eukaryotes. Due to sub-optimal amounts of nutrients in manure, supplemental feedstock materials such as Lantana camara, and Tithonia diversifolia twigs are used in composting. These materials have, however, been reported to have antimicrobial activity in in-vitro experiments. Furthermore, the phytochemical analysis has shown differences in their complexities, thus possibly requiring various periods to break down. Therefore, it is necessary to understand these materials’ influence on the biological and physical-chemical stability of compost. Most compost microbiome studies have been bacterial-centric, leaving out eukaryotes despite their critical role in the environment. Here, the influence of different green feedstock on the fungal and non-fungal eukaryotic community structure in a thermophilic compost environment was examined. Total community fungal and non-fungal eukaryotic DNA was recovered from triplicate compost samples of four experimental regimes. Sequencing for fungal ITS and non-fungal eukaryotes; 18S rDNA was done under the Illumina Miseq platform, and bioinformatics analysis was done using Divisive Amplicon Denoising Algorithm version 2 workflow in R version 4.1. Samples of mixed compost and composting day 84 recorded significantly (P<0.05) higher overall fungal populations, while Lantana-based compost and composting day 84 revealed the highest fungal community diversity. Non-fungal eukaryotic richness was significantly (P< 0.05) more abundant in Tithonia-based compost and composting day 21. The most diverse non-fungal eukaryotic biome was in the Tithonia-based compost and composting day 84. Sordariomycetes and Holozoa were the most contributors to the fungal and non-fungal community interactions in the compost environment, respectively. The findings of this study unravel the inherent influence of diverse composting materials and days on the eukaryotic community structure and compost’s biological and chemical stability.

Background Thermophilic composting is a promising method of sanitizing pathogens in manure and a source of agriculturally important thermostable enzymes and microorganisms from organic wastes. Despite the extensive studies on compost prokaryotes, shifts in microbial profiles under the influence of various green materials and composting days are still not well understood, considering the complexity of the green material sources. Here, the effect of regimens of green composting material on the diversity, abundance, and metabolic capacity of prokaryotic communities in a thermophilic compost environment was examined. Methods Total community 16S rRNA was recovered from triplicate compost samples of Lantana-based, Tithonia-based, Grass-based, and mixed (Lantana + Tithonia + Grass)- based at 21, 42, 63, and 84 days of composting. The 16S rRNA was sequenced using the Illumina Miseq platform. Bioinformatics analysis was done using Divisive Amplicon Denoising Algorithm version 2 (DADA2) R version 4.1 and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States version 2 (PICRUSt2) pipelines for community structure and metabolic profiles, respectively. In DADA2, prokaryotic classification was done using the Refseq-ribosomal database project (RDP) and SILVA version 138 databases. Results Our results showed apparent differences in prokaryotic community structure for total diversity and abundance within the four compost regimens and composting days. The study showed that the most prevalent phyla during composting included Acidobacteriota , Actinobacteriota , Bacteroidota , Chloroflexi , and Proteobacteria . Additionally, there were differences in the overall diversity of metabolic pathways but no significant differences among the various compost treatments on major metabolic pathways like carbohydrate biosynthesis, carbohydrate degradation, and nitrogen biosynthesis. Conclusion Various sources of green material affect the succession of compost nutrients and prokaryotic communities. The similarity of amounts of nutrients, such as total Nitrogen, at the end of the composting process, despite differences in feedstock material, indicates a significant influence of composting days on the stability of nutrients during composting.

IntroductionUncertainty in the yield of maize due to variability in weather is a major challenge to smallholder farmers in Sub Sahara Africa. This study explores the potential of combining locally available organic resources and inorganic fertilizer to increase grain yield and reduce variability in yields associated with variations in rainfall distribution.MethodsTo assess the effectiveness of this practice, the Agricultural Production Systems sIMulator (APSIM) crop model was calibrated and evaluated using maize experiments on nutrient management options. The evaluated model was used to simulate maize growth and yield using multiple-year data (1984–2018) under different planting windows for two growing seasons. The treatments were (i) control, (ii) inorganic fertilizer alone (INOFRecom), combining organic resources [empty fruit bunch of palm (EFB) and compost with inorganic fertilizer (INOF) to make up equivalent nutrients in (ii)]; (iii) EFB + INOF and (iv) Comp + INOF.ResultsThough all the soil amendments boosted grain output, the EFB + INOF treatment outperformed the other treatments in the major season with gains of between 161 and 211% and the most stable (least inter-annual variability of 27%) yield. Across the planting windows, the INOFRecom and EFB + INOF treatments achieved comparable yield increments in the minor season. Though grain yield variability was high during the minor rainy season, combining organic and inorganic fertilizers reduced inter-annual yield variability, thus, lowering uncertainty in yield due to variable inter-annual rainfall. Combining local organic resources with a reduced amount of inorganic fertilizer produced higher yields and better yield stability compared to using only inorganic fertilizer.DiscussionsThus, such soil fertility management solutions might sustain resource use and boost maize grain yield in the study area, where strategies for sustainable crop nutrition remain a critical necessity. The improved nitrogen management regimes may result in fewer environmental hazards for vulnerable rainfed agricultural systems.

To match Nitrogen (N) supply to crop N demand, it is essential to understand N release and uptake patterns in different farming systems and crops. To assesses the dynamics of N released in organic and conventional systems and potential synchrony and asynchrony in crop N uptake, a study was conducted over three cropping seasons (potato, maize and leafy vegetables) at two sites in the Central Highlands of Kenya. Mineral-N release and synchrony were monitored in conventional and organic systems at high (recommended N, P, pesticides and irrigation) and low input (low N, P, pesticide use and rainfed) systems. Mineral-N release was assessed using in situ buried bags and N synchrony was measured by the daily differences in N fluxes. The percentage of N applied released during potato (38%) and vegetable (44%) cropping seasons were similar between systems. However, under maize strong temporal N immobilization from inputs occurred, particularly at Thika, related to the poor quality of manure and compost (lignin:N ratio > 13). In all systems, excess-asynchrony of available N was pronounced during vegetative stages and at harvest, while insufficient-asynchrony occurred at reproductive stages. During potato cropping season at Thika, Org-High showed highest positive N fluxes (> 20 kg N ha−1 day−1) at planting and tuber bulking stage. At early stages of maize and vegetables Org-Low and Org-High experienced up to 5 times larger negative N fluxes (insufficiency) compared to conventional treatments at Chuka site. The study recommends reducing N applications at planting and increasing N dosages at reproductive stages of crops.

Purpose Market crop waste (MCW) contributes significantly to the quantity of municipal solid waste generated in sub-Saharan Africa. These wastes, however, contain high levels of plant nutrients which can be harnessed through composting to improve soil organic matter and nutrient status of impoverished tropical soils.Method In this work, annual MCWs from two urban markets in Accra, Ghana were characterized by quantifying their seasonal availabilities, primary nutrients, heavy metals concentrations and level of microbial contamination to ascertain their suitability or otherwise for composting.Results Waste generated in the rainy seasons was higher than in the dry seasons. Primary nutrients sequestered in the MCW collected were 211.1 kg N, 1.84 kg P and 89.66 kg K for both markets. Feedstock quality analysis showed heavy metal levels in the MCW were far below the contaminant levels. Moisture content of vegetable and fruit waste was 74.34 - 90.46% and far above the desired level of aerobic composting. Pathogen levels of 5.92 CFU/g E. coli and 5.41 CFU/g Salmonella in cocoyam leaves; 6.27 CFU/g total coliform and 4.74 CFU/g Enterococcus in cabbage were detected and found to be above the maximum contaminant level as per USEPA standard.ConclusionUse of cassava, plantain peduncle and corn husk as bulking agents with vegetable and fruit waste as N, P and K sources should serve as feedstock for good quality compost production.

Background A long-term experiment at two trial sites in Kenya has been on-going since 2007 to assess the effect of organic and conventional farming systems on productivity, profitability and sustainability. During these trials the presence of significant numbers of termites (Isoptera) was observed. Termites are major soil macrofauna and within literature they are either depict as ‘pests’ or as important indicator for environmental sustainability. The extent by which termites may be managed to avoid crop damage, but improve sustainability of farming systems is worthwhile to understand. Therefore, a study on termites was added to the long-term experiments in Kenya. The objectives of the study were to quantify the effect of organic (Org) and conventional (Conv) farming systems at two input levels (low and high) on the abundance, incidence, diversity and foraging activities of termites. Results The results showed higher termite abundance, incidence, activity and diversity in Org-High compared to Conv-High, Conv-Low and Org-Low. However, the termite presence in each system was also dependent on soil depth, trial site and cropping season. During the experiment, nine different termite genera were identified, that belong to three subfamilies: (i) Macrotermitinae (genera: Allodontotermes, Ancistrotermes, Macrotermes, Microtermes, Odontotermes and Pseudocanthotermes), (ii) Termitinae (Amitermes and Cubitermes) and (iii) Nasutitiermitinae (Trinervitermes). Conclusions We hypothesize that the presence of termites within the different farming systems might be influenced by the types of input applied, the soil moisture content and the occurrence of natural enemies. Our findings further demonstrate that the organic high input system attracts termites, which are an important, and often beneficial, component of soil fauna. This further increases the potential of such systems in enhancing sustainable agricultural production in Kenya.

The study was to assess the: (i) effect of human urine and other organic inputs on cabbage growth, yield, nutrient uptake, N-use efficiency, and soil chemical characteristics; (ii) economic returns of the use of urine and/or other organic inputs as a source of fertiliser for cabbage production. To meet these objectives, participatory field trials were conducted at Dzorwulu, Accra. Four different treatments (Urine alone, Urine + dewatered faecal sludge (DFS), Urine + poultry droppings (PD), NPK (15-15-15) + PD) were applied in a Randomised Complete Block Design (RCBD) with soil alone as control. Each treatment was applied at a rate of 121 kg·N·ha−1 corresponding to the Nitrogen requirement of cabbage in Ghana. Growth and yield parameters, plant nutrient uptake, and soil chemical characteristics were determined using standard protocols. There were no significant differences between treatments for cabbage head weight, or total and marketable yields. However, unmarketable yield from NPK + PD was 1 to 2 times higher (p < 0.05) than those from Urine + PD, Urine + DFS, and Urine alone. Seasonal effect on yields was also pronounced with higher (p < 0.001) cabbage head weight (0.95 kg) and marketable yields (12.7 kg·ha−1) in the dry season than the rainy season (0.42 kg and 6.27 kg·ha−1). There was higher (p < 0.005) phosphorous uptake in cabbage from Urine + PD treated soil than those from other treatments. Nitrogen (N), phosphorous (P), and potassium (K) uptake in the dry season was significantly higher than the rainy season. Soils treated with Urine + DFS and Urine + PD were high in total N content. Urine + PD and Urine + DFS treated soils gave fairly high yield than PD + NPK with a net gain of US $1452.0 and US$ 1663.5, respectively. The application of urine in combination with poultry droppings has the potential to improve cabbage yields, nutrient uptake, and soil nitrogen and phosphorous content.