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The Aromatic and Medicinal Plants sector has undergone a remarkable evolution, especially during the last decade. The global market is moving more and more towards products of natural origin. Indeed, of the 4200-existing plant in Morocco, 800 are listed as aromatic and medicinal plants. Among these plants, saffron is a source of income for many areas of Morocco. Saffron, the dried stigma of the Crocus sativus flower, is considered among the main terroir products of Morocco. Saffron has accompanied all civilizations, whether for its culinary role, for its quality of dye or its ancestral virtues rooted in folk medicine. This review highlights the main components of saffron, and the pharmacological activities that result from it and make this product a serious therapeutic hope. Then, a classification of uses of saffron was carried out according to its uses, traditional, pharmaceutical, cosmetic and perfumery without forgetting its use a spice incorporated in many dishes around the world.

Citrus species are one of the world’s popular fruit crops, cultivated all over the world for their economic and nutritional values. Citrus, like other fruits and vegetables, are an important source of several antioxidant molecules (polyphenols, ascorbic acid, and carotenoids) that can inhibit the harmful effects of free radicals on the human body; due to their functional values and health-promoting properties, Citrus species are considered valuable fruits not only in agri-food industry, but also in pharmaceutical industry. Flavonoids are among the major constituents of polyphenols found in different parts of Citrus fruits (skin, peels, seed, pulp membrane, and juice). Flavonoids have different biological properties (antiviral, antifungal, and antibacterial activities). Several studies have also shown the health-related properties of Citrus flavonoids, especially antioxidant, anticancer, anti-inflammation, anti-aging, and cardiovascular protection activities. In the present review, attempts are made to discuss the current trends of research on flavonoids in different Citrus species.

Background/Objectives: Essential oils (EOs) from Citrus species have attracted attention for their diverse properties, including anti-inflammatory, antioxidant and cytotoxic effects, which address critical health challenges such as chronic diseases and skin disorders. Citrus sinensis (L.) Osbeck, which is a widely cultivated citrus fruit, is attracting increasing attention in the field of medicinal research due to its richness of limonene (comprising approximately 85–90% of the oil). This study investigates the chemical profile of CS-EO and biological activities of CS-EO and limonene. Methods and Results: This study used gas chromatography–mass spectrometry (GC-MS), confirming limonene as the predominant compound (70.15%) along with other minor constituents, including thujene (10.52%), myrcene (5.54%) and α-pinene (2.81%). The biological activities of CS-EO and limonene were examined, specifically focusing on their antioxidant, anti-inflammatory, cytotoxicity and dermatoprotective effects. Antioxidant potential was evaluated using DPPH, FRAP and beta-carotene assays, with CS-EO and limonene exhibiting comparable efficacy. Anti-inflammatory properties were assessed via inhibition assays of prostaglandin E2 (PGE2) and nitric oxide (NO) production, showing significant reductions in LPS-stimulated macrophages treated by CS-EO or limonene. Cytotoxicity testing on various cell lines indicated selective activity of the tested compounds, with low toxicity observed on human skin fibroblasts. Limonene and CS-EO were highly effective on HepG2 cellules, with IC50 values of 0.55 ± 0.01 µg/mL and 15.97 ± 1.20 µg/mL, respectively. Dermatoprotective effects were further confirmed using enzymes, where CS-EO and limonene showed remarkable inhibitory potential against elastase (IC50 of 65.72 ± 1.92 and 86.07 ± 1.53 µg/mL, respectively) and tyrosinase (IC50 of 102 ± 2.16 and 78.34 ± 1.15 µg/mL, respectively) enzymes compared to quercetin used as a standard (IC50 of 111.03 ± 0.1 and 124.22 ± 0.07 µg/mL, respectively). Conclusions: The findings of this study suggest the potential for the development of new therapeutic approaches based on CS-EO, which could be applicable in the pharmaceutical, cosmetic and nutraceutical fields and have protective benefits for skin health.

The botanical species Ceratonia siliqua L., commonly referred to as the Carob tree, and locally as “L’Kharrûb”, holds significance as an agro-sylvo-pastoral species, and is traditionally utilized in Morocco for treating a variety of ailments. This current investigation aims to ascertain the antioxidant, antimicrobial, and cytotoxic properties of the ethanolic extract of C. siliqua leaves (CSEE). Initially, we analyzed the chemical composition of CSEE through high-performance liquid chromatography with Diode-Array Detection (HPLC-DAD). Subsequently, we conducted various assessments, including DPPH scavenging capacity, β-carotene bleaching assay, ABTS scavenging, and total antioxidant capacity assays to evaluate the antioxidant activity of the extract. In this study, we investigated the antimicrobial properties of CSEE against five bacterial strains (two gram-positive, Staphylococcus aureus, and Enterococcus faecalis; and three gram-negative bacteria, Escherichia coli, Escherichia vekanda, and Pseudomonas aeruginosa) and two fungi (Candida albicans, and Geotrichum candidum). Additionally, we evaluated the cytotoxicity of CSEE on three human breast cancer cell lines (MCF-7, MDA-MB-231, and MDA-MB-436) and assessed the potential genotoxicity of the extract using the comet assay. Through HPLC-DAD analysis, we determined that phenolic acids and flavonoids were the primary constituents of the CSEE extract. The results of the DPPH test indicated a potent scavenging capacity of the extract with an IC50 of 302.78 ± 7.55 µg/mL, which was comparable to that of ascorbic acid with an IC50 of 260.24 ± 6.45 µg/mL. Similarly, the β-carotene test demonstrated an IC50 of 352.06 ± 12.16 µg/mL, signifying the extract’s potential to inhibit oxidative damage. The ABTS assay revealed IC50 values of 48.13 ± 3.66 TE µmol/mL, indicating a strong ability of CSEE to scavenge ABTS radicals, and the TAC assay demonstrated an IC50 value of 165 ± 7.66 µg AAE/mg. The results suggest that the CSEE extract had potent antioxidant activity. Regarding its antimicrobial activity, the CSEE extract was effective against all five tested bacterial strains, indicating its broad-spectrum antibacterial properties. However, it only showed moderate activity against the two tested fungal strains, suggesting it may not be as effective against fungi. The CSEE exhibited a noteworthy dose-dependent inhibitory activity against all the tested tumor cell lines in vitro. The extract did not induce DNA damage at the concentrations of 6.25, 12.5, 25, and 50 µg/mL, as assessed by the comet assay. However, the 100 µg/mL concentration of CSEE resulted in a significant genotoxic effect compared to the negative control. A computational analysis was conducted to determine the physicochemical and pharmacokinetic characteristics of the constituent molecules present in the extract. The Prediction of Activity Spectra of Substances (PASS) test was employed to forecast the potential biological activities of these molecules. Additionally, the toxicity of the molecules was evaluated using the Protox II webserver.

Opuntia dillenii (Ker Gawl.) Haw. belongs to the Cactaceae family and is native to the arid and semi-arid regions of Mexico and the southern United States. O. dillenii are now used as medicinal plants in various countries. In this study, we investigated the chemical composition of ethanolic extracts obtained from seeds, juice, and peel of O. dillenii fruits collected from Morocco, and we evaluated their antibacterial, antifungal, and antidiabetic activities. Phytochemical screening revealed high quantities of polyphenols (193.73 ± 81.44 to 341.12 ± 78.90 gallic acid eq [g/100 g dry weight]) in the extracts. The major phenolic compounds determined by HPLC were gallic acid, vanillic acid, and syringic acid. Regarding flavonoids, quercetin 3-O-β-D-glucoside and kaempferol were the predominant molecules. Juice extracts showed weak to moderate antibacterial activity against the bacteria species Listeria monocytogenes, Escherichia coli, and Salmonella braenderup. All tested extracts displayed a significant inhibitory effect on α-glucosidase and α-amylase activities in vitro, with the peel extracts showing the greatest inhibitory effects. Together, these findings suggest that O. dillenii fruits are a promising source for the isolation of novel compounds with antibacterial or antidiabetic activities. For the most abundant phytochemicals identified in O. dillenii peel ethanolic extract, molecular docking simulations against human pancreatic α-amylase enzyme were performed. These indicated the presence of bioactive compounds in the extract with a better potential to decrease the enzyme activity than the commercial drug acarbose.

Figs are delectable, beneficial, nutritious, and seasonal; nevertheless, they deteriorate quickly, making processing the optimal method for preservation. Drying figs is the most practical and efficient way to preserve them. Paste is another possibility. The present study emphasizes the nutritional value of two fig pastes, as well as their antioxidant and antimicrobial properties. Ghoudane (GD) and Chetoui (CH) fig pastes are high in sugars, fiber, minerals, and antioxidants, making them nutritious. The HPLC-UV-MS/MS analysis identified bioactive compounds of the two fig pastes under investigation. The CH paste was found mainly to contain gallic acid, quercetin, 3’-methyl ether, and neochlorogenic acid. Similarly, the GD paste notably comprises catechin, 3’-methyl ether, and neochlorogenic acid. GD paste has more polyphenols (374.6 ± 4.57 mg GAE/100 g) and flavonoids (102.5 ± 2.16 mg QE/100 g) than CH paste. The GD paste was shown a strong antioxidant, as shown by its DPPH IC 50 value of 5.62 ± 0.02 mg/mL and TAC value of 80.16 ± 0.83 µg AAE /mg extract. Also, it revealed a strong correlation between the contents of bioactive polyphenols and flavonoids and their antioxidant and antimicrobial activities. Furthermore, hydroethanol fig paste extracts have shown high antibacterial and fungal activity. In silico analysis showed that the bioactive chemicals in both pastes demonstrated different levels of inhibition against the targeted proteins. The quercetin 3-methyl ether demonstrated the highest efficacy in blocking NADPH oxidase, with a glide gscore of -6.307 K/mol. On the other hand, neochlorogenic acid exhibited the highest efficacy in inhibiting the activity of two enzymes: diphosphate kinase from Staphylococcus aureus (-10.493 kcal/mol) and β-ketoacyl-[acyl carrier protein] synthase from Escherichia coli (-7.357 kcal/mol). Catechin is highly potent in inhibiting the pathogenic fungus Candida albicans by targeting sterol 14-α demethylase (CYP51) with a glide gscore of -7.638 kcal/mol. The fig paste is an important source of nutrients and biologically active substances that can be used in several fields, such as food and phytopharmaceuticals. This confirms the importance of the fig paste process as a way of increasing the value of the fig fruit, enhancing its utility, and thereby increasing its reputation and appreciation.

Atriplex halimus L., also known as Mediterranean saltbush, and locally as “Lgtef”, an halophytic shrub, is used extensively to treat a wide variety of ailments in Morocco. The present study was undertaken to determine the antioxidant activity and cytotoxicity of the ethanolic extract of A. halimus leaves (AHEE). We first determined the phytochemical composition of AHEE using a liquid chromatography (LC)–tandem mass spectrometry (MS/MS) technique. The antioxidant activity was evaluated using different methods including DPPH scavenging capacity, β-carotene bleaching assay, ABTS scavenging, iron chelation, and the total antioxidant capacity assays. Cytotoxicity was investigated against human cancer breast cells lines MCF-7 and MDA-MB-231. The results showed that the components of the extract are composed of phenolic acids and flavonoids. The DPPH test showed strong scavenging capacity for the leaf extract (IC50 of 0.36 ± 0.05 mg/mL) in comparison to ascorbic acid (IC50 of 0.19 ± 0.02 mg/mL). The β-carotene test determined an IC50 of 2.91 ± 0.14 mg/mL. The IC50 values of ABTS, iron chelation, and TAC tests were 44.10 ± 2.92 TE µmol/mL, 27.40 ± 1.46 mg/mL, and 124 ± 1.27 µg AAE/mg, respectively. In vitro, the AHE extract showed significant inhibitory activity in all tested tumor cell lines, and the inhibition activity was found in a dose-dependent manner. Furthermore, computational techniques such as molecular docking and ADMET analysis were used in this work. Moreover, the physicochemical parameters related to the compounds’ pharmacokinetic indicators were evaluated, including absorption, distribution, metabolism, excretion, and toxicity prediction (Pro-Tox II).

Nigella sativa L. (Ranunculaceae), commonly referred to as black seeds or black cumin, is used in popular medicine (herbal) all over the world for the treatment and prevention of several diseases, including diabetes. This study aims to investigate the inhibitory effect of N. sativa extracts and fractions against the activities of intestinal α-glucosidase and pancreatic α-amylase in vitro, and to explain the inhibitory effect of these fractions against these enzymes by identifying their active compounds responsible for this effect and determine their modes of inhibition. To do so, N. sativa hexane and acetone extracts were prepared and analyzed by GC–MS and HPLC–DAD, respectively. The hexane extract was further fractioned into eight different fractions, while the acetone extract generated eleven fractions. The extracts as well as the resulting fractions were characterized and evaluated for their potential in vitro antidiabetic activity using intestinal α-glucosidase and pancreatic α-amylase inhibitory assays in vitro. Hexane extract and fractions were less active than acetone extract and fractions. In the case of intestinal α-glucosidase activity, the acetone fraction SA3 had a high inhibitory effect on intestinal α-glucosidase activity with 72.26 ± 1.42%, comparable to the effect of acarbose (70.90 ± 1.12%). For the pancreatic α-amylase enzymatic inhibitory assay, the acetone fractions showed an inhibitory capacity close to that for acarbose. In particular, the SA2 fraction had an inhibitory effect of 67.70 ± 0.58% and was rich in apigenin and gallic acid. From these fractions, apigenin, (−)-catechin, and gallic acid were further characterized for their inhibitory actions. IC50 and inhibition mode were determined by analyzing enzyme kinetic parameters and by molecular modeling. Interestingly, (−)-catechin showed a possible synergistic effect with acarbose toward α-glucosidase enzyme inhibition, whereas apigenin showed an additive effect with acarbose toward α-amylase enzymatic inhibition. Furthermore, we studied the toxicity of N. sativa hexane and acetone extracts as well as that of acetone fractions. The result of acute toxicity evaluation demonstrated that N. sativa extracts were nontoxic up to a concentration of 10 g/kg, except for fraction SA3. Taken together, these results indicate that N. sativa extracts and/or derived compounds could constitute promising nutraceuticals for the prevention and treatment of type 2 diabetes mellitus.

Kidney disease is one of the most common health problems and kidney failure can be fatal. It is one of the health disorders associated with extreme pain and discomfort in patients. In developing countries, such as Morocco where socioeconomic and sanitary conditions are precarious, medicinal plants are considered the primary source of medication. In the present work an ethnobotanical survey was conducted in a remote area of North-Eastern Morocco and we focused on (1) establishing a record of medicinal plants used traditionally by local people to treat kidney diseases and (2) correlate the obtained ethnomedical use with well-studied pharmacological evidence. From February 2018 to January2020, information was gathered from 488 informants using semi-structured questionnaires. The data were analyzed using three quantitative indices: The use value (UV), family use value (FUV), and informant consensus factor (ICF). A total of 121 plant species belonging to 57 botanical families were identified to treat kidney diseases. The families most represented were Asteraceae (14 species), followed by Lamiaceae (12 species) and Apiaceae (10 species). The most commonly used plant parts were leaves, followed by the whole plant and they were most commonly prepared by decoction and infusion. The highest value of the (UV) index was attributed to Herniaria hirsuta L. (UV = 0.16), and the highest family use value (FUV) was assigned to Caryophyllaceae with (FUV = 0.163). Regarding the informant consensus factor (ICF), this index’s highest values were recorded for kidney stones (ICF = 0.72). The use of 45% of the selected plants were validated based on literature review. This study helped document and preserve crucial traditional plant knowledge of 121 plant species used to treat kidney problems that can be used in the search for new biologically active compounds through more upcoming pharmacological studies.

The fig tree is a multifaceted plant with a wide range of nutritional and health benefits. The employment of the Aksoy weighted classification method, predicated on biological characteristics pertaining to the ripening period, in conjunction with pomological and chemical characteristics of the fresh fruit, yielded favorable outcomes in the assessment of fresh fig quality from eastern Morocco, particularly the OnK Hmam (718) and Chetoui (720) varieties, indicating high quality. From a commercial perspective, the weight of figs is a key factor in determining their value. The study examined the weight of five different varieties of fresh figs, revealing significant variations in their average weight. The Malha fig recorded the lowest average weight of 30.24 g, while the Onk Hmam fig exhibited the highest average weight of 57.25 g. Fresh Chetoui has the highest content of total sugar (15.04%), titratable acid (0.272%), potassium (266.8 mg/100 g), sodium (0.580 mg/100 g), calcium (75.20 mg/100 g) and phosphorus (30.04 mg). Fresh Onk Hmam has the highest fibre concentration (2.60%). Fresh Bounacer has the highest magnesium content (20.52 mg/100 g). Fresh Ghoudane has the highest concentration of iron (1.24 mg/100 g), polyphenols (275.6 GAE/100 g FW) and flavonoids (120.3 QE/100 g FW) among the samples. As fresh figs are seasonal and perishable, the most effective method of preserving their nutritional content and health benefits over an extended period is through the drying process. The primary objective of the drying process is to dehydrate the figs and reduce their water activity to an acceptable level. In addition, the storage and transport costs incurred are economically and commercially advantageous. Sun-dried goudane and chetoui are characterized by their elevated sugar, fiber, mineral, and antioxidant content. Additionally, they exhibit significant volume and weight reduction, thereby reducing packaging requirements and rendering them optimal for use as dietary supplements. In addition, they meet the required criteria of ≤ 26.0% for dry matter and the minimum diameter of 18 mm for black fig (Ghoudane) and 22 mm for white fig (Chetoui) of the European standards. However, it should be noted that their marketability and commercial quality are limited to Category I (65 < maximum allowable fruit count per kilogram ≤ 120), which includes lower quality designations. Dried Ghoudane was found to contain higher levels of polyphenols (380.9 mg GAE/100 g DW) and flavonoids (100.1 mg QE/100 g DW) than dried Chetoui (305.9 mg GAE/100 g DW and 81.90 mg EQ/100 g DW). After sun drying of fresh Ghoudane and Chetoui, Principal Component Analysis (PCA) showed that they had increased levels of phenolic content, titratable acid and minerals. However, total flavonoid content, vitamin C content and soluble solids decreased.