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Background Our aim was to investigate whether the surgical approach, i.e. endoscopic (ET) versus conventional/open thyroidectomy (ConT), contributes towards the improvement in quality of life (QoL), cosmetic outcomes and overall patient satisfaction with treatment. Methods We conducted a prospective study (October 2016–August 2018) consisting of patients undergoing hemithyroidectomy for euthyroid, non-malignant solitary thyroid nodules (STNs). Patients were divided into two groups: Group I (ET)—41 patients and Group II (ConT)—52 patients. ET was performed via bilateral-axillo breast approach (BABA). A thyroid disease-specific questionnaire “ThyPRO-39hin” was used to evaluate pre- and post-operative QoL. Visual analogue scale (VAS) was used for post-operative pain. Cosmetic satisfaction, paraesthesia and overall satisfaction with treatment were evaluated using Likert-type verbal response scales. Pre- and post-operative scores (>6 months) were compared, and p value < 0.05 was considered significant. Results Mean age ( p  = 0.26), gender distribution ( p  = 0.07), mean tumour size ( p  = 0.74) and preoperative scores of QoL were comparable between the two groups. Post-operatively scores of symptom domain ( p  = 0.03), tiredness ( p  = 0.03), impaired social life ( p  = 0.03), cosmetic complaints ( p  =  < 0.001) and overall QoL ( p  =  < 0.001) were significantly better in Group I. Also, post-operative pain perception at first follow-up visit ( p  = 0.001) was lower in patients undergoing ET/Group I, with higher scar satisfaction scores ( p  =  < 0.001) and overall satisfaction with treatment ( p  =  < 0.001). Post-operative paraesthesia perception was comparable ( p  = 0.06) amongst the two groups. Conclusion In this cohort study, patients undergoing endoscopic thyroid surgery reported superior post-operative QoL, cosmetic and overall satisfaction with treatment as compared to conventional/open thyroidectomy.

HuD plays a critical role in neurite outgrowth, neuronal plasticity, and survival. However, HuD autoantibodies from patients with paraneoplastic gut dysmotility can trigger the apoptotic cascade in human neuroblastoma cell line and myenteric neurons. The mechanism by which HuD regulates the apoptotic pathway is unclear. Apoptosis is one of the underlying causes of neurodegenerative diseases like Alzheimer’s disease. In the current study, we found that HuD interacts with Msi2 transcript and positively regulates it in the mouse neuroblastoma (N2a) cells. MSI2 being an RNA binding protein has diverse mRNA targets and regulates the mitochondrial apoptotic pathway by interacting with and repressing APAF1 transcript. Conversely, the reduced levels of HuD leads to decreased Msi2 expression and increased APAF1 levels, which results in apoptosis in N2a cells. Overall, our research indicates that HuD and Msi2 possess an anti-apoptotic role in N2A cells.

HuD plays a critical role in neurite outgrowth, neuronal plasticity, and survival. However, HuD autoantibodies from patients with paraneoplastic gut dysmotility can trigger the apoptotic cascade in human neuroblastoma cell line and myenteric neurons. The mechanism by which HuD regulates the apoptotic pathway is unclear. Apoptosis is one of the underlying causes of neurodegenerative diseases like Alzheimer's disease. In the current study, we found that HuD interacts with Msi2 transcript and positively regulates it in the mouse neuroblastoma (N2a) cells. MSI2 being an RNA binding protein has diverse mRNA targets and regulates the mitochondrial apoptotic pathway by interacting with and repressing APAF1 transcript. Conversely, the reduced levels of HuD leads to decreased Msi2 expression and increased APAF1 levels, which results in apoptosis in N2a cells. Overall, our research indicates that HuD and Msi2 possess an anti-apoptotic role in N2A cells.

Background There is limited experience in managing pediatric primary hyperparathyroidism (PHPT). The aim of this study was to analyze the clinical presentation and outcome of surgery in children with PHPT managed at a tertiary referral center. Methods This retrospective study (September 1989–August 2019) consisted of 35 pediatric PHPT patients (< 18 years) who underwent parathyroidectomy. Clinico-pathologic profile and outcome were noted. Results The mean age of cohort was 15.2±2.9 years and girls outnumbered boys (M:F = 1:1.9). Familial and symptomatic disease was noted in 8.5 and 94.3% cases, respectively. Skeletal manifestations (83%) were the commonest followed by renal (29%). Fifty-four percent children had skeletal fractures, and 23% were bed-ridden. Among rare manifestations, hypercalcemic crisis, recurrent pancreatitis and stigmata of rickets were observed in 2.8, 11.4 and 14.2% children, respectively. Mean calcium concentration was 12.1 ± 2.0 mg/dl and PTH 91.8 ± 66.5 pmol/L. The sensitivity of preoperative imaging in parathyroid localization was 91.4%. Minimally invasive parathyroidectomy (MIP) was performed in 40% cases. Parathyroid adenoma was observed in 91.4% patients, whereas remaining had hyperplasia. Thirty-four percent suffered from Hungry bone syndrome in postoperative period. The cure rate following primary surgery was 97%. One child with persistent PHPT had successful re-operation. Median follow-up was 5 (1–17) years, and no recurrence or familial disease was revealed during this period. Conclusion Majority of pediatric patients present with symptomatic PHPT. Despite relatively high incidence of familial disease select pediatric patients can undergo successful MIP.

We propose Pseudomonas coronafaciens sp. nov. as a new species in genus Pseudomonas, which is diverse from P. syringae. We also classified strains from onions which are responsible for yellow bud (YB) disease as P. coronafaciens. Sequencing of 16S rRNA gene and multi-locus sequence analysis (MLSA) of housekeeping genes (gyrB, rpoD, gltA and gap1 genes) for the P. syringae pv. coronafaciens strains along with other strains of P. syringae pathovars resulted in a distinct cluster separate from other P. syringae pathovars. Based on DNA-DNA relatedness, pathotype strain of P. syringae pv. coronafaciens (CFBP 2216PT) exhibited ≤35.5% similarity with the pathotype strains of P. syringae pv. syringae (CFBP 1392PT, 4702T) but exhibited ≥90.6% with the YB strains (YB 12-1, YB 12-4, YB 09-1). Also, the YB strains (YB 12-1, YB 12-4, YB 09-1) were able to infect only onion but not oat, rye and Italian ryegrass (common hosts for P. syrinage pv. coronafaciens). Contrastingly, P. syringae pv. coronafaciens strains (NCPPB 600PT, ATCC 19608, Pcf 83-300) produced typical halo blight symptoms on oat, rye and Italian rye grass but did not produce any symptoms on onion. These results provide evidence that P. syringae pv. coronafaciens should be elevated to a species level and the new YB strains may potentially be a novel pathovar of hereto proposed P. coronafaciens species.

INTRODUCTION: The quantity of audio and visual data is increasing exponentially due to the internet's rapid growth. The digital information in images and videos could be used for fully automated captions, indexing, and image structuring. The online image and video data system has seen a significant increase. In such a dataset, images and videos must be retrieved, explored, as well as inspected.OBJECTIVES: Text extraction is crucial for locating critical as well as important data. Disturbance is indeed a critical factor that affects image quality, and this is primarily generated during image acquisition and communication operations. An image can be contaminated by a variety of noise-type disturbances. A text in the complex image includes a variety of information which is used to recognise textual as well as non-textual particulars. The particulars in the complicated corrupted images have been considered important for individuals seeing the entire issue. However, text in complicated degraded images exhibits a rapidly changing form in an unconstrained circumstance, making textual data recognition complicated.METHODS: The naïve bayes algorithm is a weighted reading technique is used to generate the correct text data from the complicated image regions. Usually, images hold some disturbance as a result of the fact that filtration is proposed during the early pre-processing step. To restore the image's quality, the input image is processed employing gradient and contrast image methods. Following that, the contrast of the source images would be enhanced using an adaptive image map. Stroke width transform, Gabor transform, and weighted naïve bayes classifier methodologies have been used in complicated degraded images to segment, features extraction, and detect textual and non-textual elements.RESULTS: Finally, to identify categorised textual data, the confluence of deep neural networks and particle swarm optimization is being used. The dataset IIIT5K is used for the development portion, and also the performance of the suggested methodology is assessed by utilizing parameters like as accuracy, recall, precision, and F1 score. It performs well enough for record collections such as articles, even when significantly distorted, and is thus suitable for creating library information system databases.CONCLUSION: A combination of deep neural network and particle swarm optimization is being used to recognise classified text. The dataset IIIT5K is used for the development portion, and while high performance is achieved with parameters such as accuracy, recall, precision, and F1 score, characters may occasionally deviate. Alternatively, the same character is frequently extracted [3] multiple times, which may result in incorrect textual data being extracted from natural images. As a result, an efficient technique for avoiding such flaws in the text retrieval process must be implemented in the near future.

Background Sentinel lymph node biopsy (SLNB) using radio-pharmaceutical (RP) and a blue dye is gold standard for axillary staging in clinically node-negative early breast cancer. High costs and limited availability of RP and/or gamma probe are major deterrents in performing SLNB in developing countries. Fluorescence-guided SLNB can obviate the need for RP and gamma probe. Fluorescein is an inexpensive fluorescent lymphatic tracer. In this study, we compared SLN identification rate (SLN-IR) and false negative rates (FNR) of fluorescein-guided SLNB and radio-guided SLNB using 99m Tc-Sulfur-colloid, in isolation, or in combination with methylene blue dye (MBD). Methods Sixty-five cN0 early and large operable breast cancer patients underwent validation SLNB using fluorescein (and blue LED light), 99m Tc-Sulfur-colloid (and gamma probe) and MBD. Inj Fluorescein 4% was injected, 1 ml each peri-tumoral and sub-areolar five minutes before axillary incision. Axillary dissection was performed irrespective of SLNB histology. The SLN-IR and FNR with various tracers and their combinations were compared. Results The mean number of SLNs identified was 3.5 ± 1.8 (range 1–6). The SLN-IR using RP alone was 94%, fluorescein alone was 92%, and MBD alone was 82%. The SLN-IR using fluorescein plus MBD combination was 95.4%, compared to 97% using MBD plus RP combination. FNR was 6.3% using fluorescein plus MBD, as well as RP plus MBD combinations. Conclusions SLN-IR of > 90% and SLN-FNR of < 10% using fluorescein plus MBD combination are in acceptable range, and are comparable to that of RP plus MBD combination. Fluorescein can replace RP for performing SLNB, in combination with MBD.

Subgenome dominance has been reported in diverse allopolyploid species, where genes from one subgenome are preferentially retained and are more highly expressed than those from other subgenome(s). However, the molecular mechanisms responsible for subgenome dominance remain poorly understood. Here, we develop genome-wide map of accessible chromatin regions (ACRs) in cultivated strawberry (2 n  = 8 x  = 56, with A, B, C, D subgenomes). Each ACR is identified as an MNase hypersensitive site (MHS). We discover that the dominant subgenome A contains a greater number of total MHSs and MHS per gene than the submissive B/C/D subgenomes. Subgenome A suffers fewer losses of MHS-related DNA sequences and fewer MHS fragmentations caused by insertions of transposable elements. We also discover that genes and MHSs related to stress response have been preferentially retained in subgenome A. We conclude that preservation of genes and their cognate ACRs, especially those related to stress responses, play a major role in the establishment of subgenome dominance in octoploid strawberry. Subgenome dominance is widely observed in allopolyploid species, but the molecular mechanisms remain unclear. Here, the authors generate genome-wide map of accessible chromatin regions (ACRs) in allo-octoploid cultivated strawberry and reveal that dynamics of the ACRs play an important role in its subgenome dominance.