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"Ahmed, Jaseem"
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Experimental study and analysis on single slope single basin solar still with zeolite crystals as storage materials
We humans find solutions for every problem around us. Taking an environmental problem for water scarcity we have come up with a traditional idea but a bit modified. We have used solar distillation as the basic concept and developed a single slope single basin solar still (SSSBSS) where we have used a storage material that is rarely used for this process and have carried out the research work in it. The experimental study is carried for a single slope single basin solar still (SSSBSS) under two cases (i) SSSBSS (ii) SSSBSS with zeolite crystals as storage materials. The still was made of galvanized iron (GI) and the sides were insulated with polystyrene. It has a basin area of 1 m 2 . Bore water of Coimbatore which is a bit salty was taken as the feed water. The test was carried out at Kuniyamuthur, Coimbatore during 10 th , 11 th , 12 th of February 2021. Even though it was winter, the output was average. By the comparison using zeolite crystals, the efficiency was doubled compared to the first case.
Journal Article
Why Fed's strategy on liquidity risk deserves respect
Sir, With reference to your editorial advice to the Fed (\"Ignore market fear\", August 8), in relation to hedge funds, the Fed and its sister regulators closely supervise systemically important institutions - commercial and investment banks - at the core of the US financial system.
Newspaper Article
X-linked ADGRG2 mutation and obstructive azoospermia in a large Pakistani family
We performed whole exome sequencing to identify an unknown genetic cause of azoospermia and male infertility in a large Pakistani family. Three infertile males were subjected to semen analysis, hormone testing, testicular histology, ultrasonography, karyotyping, Y-chromosome microdeletion and
CFTR
testing. The clinical testing suggested a diagnosis of obstructive azoospermia (OA). To identify the cause, we performed whole exome sequencing (WES) for 2 infertile brothers and 2 fertile family members. For segregation analysis and variant confirmation, we performed Sanger sequencing. WES data analysis of the family revealed segregated variants in 3 candidate genes. We considered novel nonsense variant c.2440C > T(p.Arg814*) in X-linked gene
ADGRG2
as biologically most plausible. It is predicted to truncate the protein by 204 amino acids (aa) at a key transmembrane domain.
Adgrg2
-knockout male mice show sperm loss due to obstructive fluid stasis, while
ADGRG2
mutations cause OA in the infertile male patients. Our analysis of testicular histology reveals secondary severe reduction of spermatogenesis, consistent with human and knockout mouse phenotypes. The
ADGRG2
nonsense mutation is absent in the largest population databases, ExAC and gnomAD. Analysis of the novel nonsense mutation in extended family members confirmed co-segregation of the mutation with OA in all affected males. The likely pathogenic nature of the mutation is supported by its truncation effect on the transmembrane domain and distinctive ultrasound results. The study demonstrates effectiveness of WES in discovering a genetic cause of azoospermia.
Journal Article
Influence of NPK Fertilizer, Nano-Selenium and Silicon Oxide Nanoparticles of Two Different Cultivars of Grape (Vitis vinifera L.) on Some Chemical Characteristics
This research was carried out in one of the private orchards in the Al-Midihatiah District, Babylon Governorate. An experiment was conducted on two different cultivars of European grapes Khalili and Halwani to study the influence of NPK fertilizer, nano-selenium and silicon oxide and their effect on some chemical contents (nitrogen, phosphorus, potassium, phenols and flavones). Factorial experiment was done according to the Randomized Complete Block Design (RCBD) (6×3×2). It included two different cultivars (Khalili and Halwani) and three levels of NPK fertilizer (0, 1.5, 3 mL L −1 ) and six levels of Se and SiO 2 and their interactions [C1: 0 Se and 0 SiO 2 , C2: 15 Se, C3: 30 Se, C4: 50 SiO 2 , C5: 100 SiO 2 , C6: 30 Se+100 SiO 2 g L −1 ]. Results showed a significant effect of triple interference on the percentage of nitrogen, phosphorus and potassium where the highest value of the Khalili cultivar was reached when using NPK3 and 50 silicon, compared to the lowest value when treating NPK0 and 15 selenium for the Khalili cultivar. There was a significant effect in the characteristics of the contents of the leaves of phenols and flavones where the highest values were observed when using the concentration NPK3 g L −1 and 30 selenium + 100 silicon for the Khalili cultivar.
Journal Article
Manufacturing and studying the effect of partial substitution on the properties of the compound Bi2-x AgxSr1.9Ba0.1Ca2Cu3O10+δ superconductors
The effect of the silver fractional exchange at Bi sites on the structure, electric, morphologic and δ content for Bi-2223 have been thoughtful. High temperature superconductor composition (Bi2-x AgxSr1.9Ba0.1Ca2Cu3O10+δ ) with (x = 0.1 - 0.4) which preparing by method of SSR . XRD diffraction technique was used to estimate the structural properties of the specimens which show an orthorhombic crystalline structure for all the specimens with elevated rate of Bi-2223 phase and growing of c-axis lattice parameter with growing Ag rate. By using AFM in 3D the surface morphology has been thoughtful. The results of all samples specimens showed that have homogeneous surface and good crystalline. Tc measured by using 4-probe technique. The highest Tc was noted to be Tc = 138 K for x = 0.4, and oxygen contend was observed increase with increase Ag concentration.
Journal Article
Whole Genome Sequencing instead of Whole Exome Sequencing is required to identify the Genetic Causes of Polycystic Ovary Syndrome in Pakistani families
Background & Objective: Polycystic Ovary Syndrome (PCOS) is the major cause of infertility in females. PCOS is a complex and multifactorial disease, genetic and environmental factors being important predisposing factors. Diagnosis of PCOS is difficult due to the complexity of this disease; hence, better diagnostic tests are required to improve its management. Aim of the study was to elucidate the genetic causes of PCOS in three Pakistani families.Methods: Three Pakistani families segregating PCOS in an apparently autosomal recessive mode were recruited. Whole genome Single Nucleotide Polymorphism (SNP) genotyping and Whole Exome Sequencing (WES) were carried out to identify the candidate genes.Results: SNP genotypes data analyses identified multiple regions of homozygosity on different chromosomes. WES was performed in affected members of the family. Screening for pathogenic mutations in homozygous regions failed to detect any mutation/variant of interest.Conclusion: PCOS is multifactorial and complex disease so variants in the coding as well as in non-coding regions may be the genetic causes of the disease. To elucidate the genetic cause(s) of the PCOS, Whole Genome Sequencing (WGS) is recommended to cover both coding and non?coding regions of the genome.doi: https://doi.org/10.12669/pjms.343.14644How to cite this:Khan MJ, Nazli R, Ahmed J, Basit S. Whole Genome Sequencing instead of Whole Exome Sequencing is required to identify the Genetic Causes of Polycystic Ovary Syndrome in Pakistani families. Pak J Med Sci. 2018;34(3):540-545.
Journal Article
PENILE NEUROFIBROMA IN A 10-YEAR - OLD CHILD: A RARE CASE STUDY
Neurofibromas of the penis occur uncommonly and their solitary occurrence is even rarer. We report a case of a ten year old child presenting with a solitary neurofibroma located on penis. Aprovisional clinical diagnosis of penile retention cyst was made and histopathological examination releaved a benign spindle cell lesion and few close differential diagnosis were made, which on application of immunohistochemistry was diagnosed as neurofibroma of penis. Surgical excision of the lesion was done and there was no recurrence after a year of follow up.
Journal Article