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In this study, the nutritional and phytochemical components of red seaweed, Gracilariopsis longissima, were assessed in relation to the effects of various drying methods (freeze, oven, and sun). Gracilariopsis longissima’s proximate composition differed significantly (P < 0.05) among the three drying techniques, with freeze-dried samples having significantly greater protein (30.63 ± 0.90% dry weight) and lipids (1.49 ± 0.05% dry weight) contents. Except for phosphorus and sulfur, the mineral concentrations were likewise considerably greater in the freeze-dried samples. The total amino acids (30.48 ± 0.06% dry weight) and fatty acids contents were substantially greater (P < 0.05) in the freeze-dried samples. In freeze-dried samples, the levels of essential amino acids (18.92 ± 0.02% dry weight) and unsaturated fatty acids (54.08 ± 0.07%) were substantially greater (P < 0.05). When compared to oven- and sun-dried samples, the total phenolic content (88.70 ± 2.19 mg GAE/g dry weight) and the flavonoid content (71.46 ± 2.17 mg QE/g dry weight) of freeze-dried samples was also substantially higher. Among the three distinct drying techniques used, the freeze-drying technique kept G. longissima’s higher nutritional and phytochemical components.

Seaweed is known to empower a mass embodiment of bioactive compounds with potent health convenience. Current perusals intended to explore the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant dynamism of red seaweed, Gracilariopsis longissima as pervaded by different solvents and solvent fractions (methanol, ethanol, acetone, and water). The extracts dynamism to antioxidant were assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2, 2 ’ -Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Here, 100% methanolic and acetonic extract asserted maximal extent of total phenolic and flavonoid content (88.70 mg of gallic acid/g and 75.06 mg of quercetin/g respectively) in a sufficiently great way. However, 50% methanolic DPPH and ABTS solution (74.32%, IC 50  = 0.027 mg/ml and 70.51%, IC 50  = 0.033 mg/ml) significantly demonstrated the highest percentage of inhibition and antioxidant activity compared to other solvents. Gracilariopsis longissima also contains a high amount of protein (30.63%) and minerals especially potassium (4.47%) and calcium (2.54%). According to the results of this study, G. longissima extracts prepared using 100% methanol as a solvent showed higher total phenolic and total flavonoid quantity and 50% methanol as a solvent showed higher antioxidant potential. These properties will be valuable for nutritional food and medicinal uses.

This research was aimed at utilizing available resources and evaluating the effectiveness of value‐added metrics for fish‐based products, such as fish balls made from various types of fish mince and small tuna (Euthynnus affinis). The fish balls were stored using two distinct approaches: deep freezing (−18°C) for 60 days and refrigeration (4°C). The gustatory approach and the AOAC method were used to gauge the nutritional content and aesthetic effectiveness of the fish products, respectively. The deep‐frozen samples exhibited improved crispness compared to those stored in the refrigerator. Deep‐frozen fish balls had substantially lower pH, total volatile base‐nitrogen (TVB‐N), trimethylamine nitrogen (TMA‐N), and histamine concentrations during the storage period ( p < 0.05) than chilled fish balls. Salmonella species were not detected during the diagnostic process, while the Escherichia coli plate count in the deep‐frozen fish balls significantly decreased to just under 10 5 cfu/g. Fish mince‐based value‐added seafood products could help Bangladesh utilize its resources more efficiently and contribute to maintaining sustainable food production. Furthermore, minimal adverse nutritional effects were observed, and the tuna fish balls remained safe for consumption after storage at −18°C for 60 days.

This investigation evaluated the therapeutic benefit of apocynin in isoproterenol (ISO)‐induced cardiac damage in rats. ISO‐administered male Wistar rats were treated with apocynin for 2 weeks. Blood plasma and left ventricle of heart tissues were collected and analyzed for oxidative stress‐related parameters such as malondialdehyde (MDA), advanced oxidation protein product (AOPP), and nitric oxide (NO). The activities of endogenous antioxidant enzymes such as superoxide dismutase (SOD) and catalase were also measured. The gene expressions of oxidative stress‐related proteins such as Nrf‐2, HO‐1, and HO‐2 in cardiac tissues were also measured. In silico studies like molecular docking and molecular dynamics were also performed to detect how apocynin interacts with NADPH and nitric oxide synthase at the molecular level. This investigation revealed significant elevation of serum transferase enzymes and creatinine kinase‐Muscle Brain (CK‐MB) activities in ISO‐administered rats compared to the control. Apocynin effectively normalized the serum transferases and CK‐MB activities in the blood of ISO‐stressed rats. Moreover, ISO‐induced elevations of MDA, NO, and AOPP levels were also suppressed by apocynin treatment. Consistently, apocynin restored the reduced SOD and catalase activities in ISO‐administered rats. This restoration of enzyme activity might be due to the increased expression of Nrf‐2 and HO‐1 and reduced expression of iNOS and TNF‐α in ISO‐administered rats. Histological analysis revealed that apocynin treatment ameliorated the mononuclear cell adherence and fibrosis in the cardiac tissue of ISO‐administered rats. Computational studies also support the experimental findings. This study demonstrates that apocynin prevents ISO‐induced cardiac injury not only by preventing inflammation but also by empowering the antioxidant defense system. This study suggests that apocynin protects ISO‐induced heart damage by preventing inflammation and fortifying the antioxidant defense system.