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The life satisfaction of women is an essential component of their subjective well-being. It is an indicator of a woman’s life quality based on personal perception. Considering the importance of women’s subjective well-being, the United Nations (UN) has recognized this as one of its Sustainable Development Goals (SDGs). To the best of our knowledge, no study has been done yet for Bangladeshi women aged 15-49 years using nationally represented data. This study is the first study, to our knowledge, that will identify the determinants of life satisfaction and investigate the association between these determinants and life satisfaction among women of reproductive age (15-49 years) in Bangladesh. This study examined the overall life satisfaction and impact of some personal and sociodemographic characteristics on it among the women aged 15 to 49 years in Bangladesh using the Multiple Indicator Cluster Survey (MICS) 2019 data, a cross-sectional data. In our study, information regarding life satisfaction was available for a sample of 64,283 women after categorizing the variable overall life satisfaction and adjusting the missing values. The variable overall life satisfaction was categorized into three categories, namely low (0-3), moderate (4-6), and high (7-10), according to the Organisation for Economic Co-operation and Development (OECD) guidelines. Results showed that 14.67% of women are low satisfied, 50.65% are moderately satisfied, and 34.68% are highly satisfied with their lives. A bivariate analysis was applied in this study to show the significant association between the determinants and life satisfaction. The multinomial logistic regression analysis was performed to draw valid conclusions about the effects of the potential determinants on life satisfaction. The study revealed that satisfaction increases with age, education level, and wealth status. For the variable marital status, which has three categories: currently married, formerly married, and never married, current marriage was strongly associated with higher life satisfaction. In contrast, a former marriage and a never marriage were associated with lower life satisfaction. Migration status was also significantly associated with life satisfaction, where women who changed their location were more satisfied than those who never changed their current location. Bangladesh aims to achieve the Sustainable Development Goals (SDGs) by 2030. To achieve the Sustainable Development Goal (SDG) 3, which promotes well-being, and the goal 5, to empower all women and girls, addressing the issues related to life satisfaction and applying appropriate intervention is a must.

ObjectiveThe major objective of this project is to find the best suitable model for district-wise infant mortality rate (IMR) data of Bangladesh over the period 2014–2020 that captures the regional variability and overtime variability of the data.Design, setting and participantsData from seven consecutive cross-sectional surveys that were conducted in Bangladesh between 2014 and 2020 as a part of the Sample Vital Registration System (SVRS) were used in this study. The study included a total of 13 173 (with 390 infant deaths), 17 675 (with 512 infant deaths), 17 965 (with 501 infant deaths), 23 205 (with 556 infant deaths), 23 094 (with 498 infant deaths), 23 090 (with 497 infant deaths) and 23 297 (with 495 infant deaths) complete cases from SVRS datasets for each respective year.MethodA linear mixed effects model (LMM) with a quadratic trend over time in the fixed effects part and a nested random intercept, as well as a nested random slope for a linear trend over time in the part of the random effect, was implemented to describe the situation. This model was selected based on two popular selection criteria: Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC).ResultsThe LMMs analysis results demonstrated statistically significant variations in IMR across different districts and over time. Examining the district-specific area under the logarithm of the IMR curves yielded valuable insights into the disparities in IMR among different districts and regions. Furthermore, a significant inverse relationship was observed between IMR and life expectancy at birth, underscoring the significance of mitigating IMR as a means to enhance population health outcomes.ConclusionThis study accentuates district-wise and temporal variability when modelling IMR data and highlights regional heterogeneity in infant mortality rates in Bangladesh. Area-based programmes should be created for mothers residing in locations with a higher risk of IMR. Further research can examine socioeconomic elements generating these discrepancies.

Recognizing cholera cases early, especially in the initial phase of an outbreak and in areas where cholera has not previously circulated, is a high public health priority. Laboratory capacity in such settings is often limited. To address this, we have developed a rapid diagnostic test (RDT) termed Cholkit that is based on an immunochromatographic lateral flow assay for the diagnosis of cholera cases using stool. Cholkit contains a monoclonal antibody (ICL-33) to the O-specific polysaccharide (OSP) component of V. cholerae O1 lipopolysaccharide, and recognizes both Inaba and Ogawa serotypes. We tested the Cholkit dipstick using fresh stool specimens of 76 adults and children presenting with acute watery diarrhea at the icddr,b hospital in Dhaka, Bangladesh. We compared Cholkit's performance with those of microbial culture, PCR (targeting the rfb and ctxA genes of V. cholerae) and the commercially available RDT, Crystal VC (Span Diagnostics; Surat, India). We found that all stool specimens with a positive culture for V. cholerae O1 (n = 19) were positive by Cholkit as well as Crystal VC. We then used Bayesian latent class modeling to estimate the sensitivity and specificity of each diagnostic assay. The sensitivity of Cholkit, microbiological culture, PCR and Crystal VC was 98% (95% CI: 88-100), 71% (95% CI: 59-81), 74% (95% CI: 59-86) and 98% (95% CI: 88-100), respectively. The specificity for V. cholerae O1 was 97% (95% CI: 89-100), 100%, 97% (95% CI: 93-99) and 98% (95% CI: 92-100), respectively. Of note, two Crystal VC dipsticks were positive for V. cholerae O139 but negative by culture and PCR in this area without known circulating epidemic V. cholerae O139. In conclusion, the Cholkit dipstick is simple to use, requires no dedicated laboratory capacity, and has a sensitivity and specificity for V. cholerae O1 of 98% and 97%, respectively. Cholkit warrants further evaluation in other settings.

Habb-e-Suranjan (HES), an Unani formulation, has been studied for its anti-inflammatory properties in both in vitro and in vivo experiments. HES is recommended for arthritis, gout, and joint pain. The current endeavor is an attempt to put it to the test and verify its efficacy scientifically. It was tested for DPPH, hydroxyl, and nitric oxide scavenging activities. It was shown that HES had the greatest TAC and FRAC values when compared to catechin and ascorbic acid. HES exhibited DPPH and hydroxyl radical scavenging activity that was dose-dependent. Incubation of sodium nitroprusside solutions in PBS at 25°C for 150 min resulted in the production of nitric oxide. Nitric oxide production was effectively decreased by HES. Anti-inflammatory medications boosted the migration of PMN cells toward the chemoattractant FMLP in an agarose experiment of PMN chemotaxis. In carrageenan-induced rat paw edema, in the HES-treated group, paw thickness was 3.021±0.084 at t=0, but it showed an increase in paw inflammation after one hour, i.e., 3.195±0.082 cm which again showed a decrease in paw thickness up to 4th hour, i.e., 3.018±0.078, 2.98±0.032, and 2.684±0.061 at t=2, 3, and 4, respectively. It showed again getting back to the normal thickness of paw at t=24 hrs, i.e., 3.029±0.118 cm. It is concluded that the formulation is potent enough and can be used effectively for the treatment of inflammation and associated health issues. Moreover, there is much scope to evaluate its effectiveness using different in vitro and in vivo models.

The life satisfaction of women is an essential component of their subjective well-being. It is an indicator of a woman's life quality based on personal perception. Considering the importance of women's subjective well-being, the United Nations (UN) has recognized this as one of its Sustainable Development Goals (SDGs). To the best of our knowledge, no study has been done yet for Bangladeshi women aged 15-49 years using nationally represented data. This study is the first study, to our knowledge, that will identify the determinants of life satisfaction and investigate the association between these determinants and life satisfaction among women of reproductive age (15-49 years) in Bangladesh. This study examined the overall life satisfaction and impact of some personal and sociodemographic characteristics on it among the women aged 15 to 49 years in Bangladesh using the Multiple Indicator Cluster Survey (MICS) 2019 data, a cross-sectional data. In our study, information regarding life satisfaction was available for a sample of 64,283 women after categorizing the variable overall life satisfaction and adjusting the missing values. The variable overall life satisfaction was categorized into three categories, namely low (0-3), moderate (4-6), and high (7-10), according to the Organisation for Economic Co-operation and Development (OECD) guidelines. Results showed that 14.67% of women are low satisfied, 50.65% are moderately satisfied, and 34.68% are highly satisfied with their lives. A bivariate analysis was applied in this study to show the significant association between the determinants and life satisfaction. The multinomial logistic regression analysis was performed to draw valid conclusions about the effects of the potential determinants on life satisfaction. The study revealed that satisfaction increases with age, education level, and wealth status. For the variable marital status, which has three categories: currently married, formerly married, and never married, current marriage was strongly associated with higher life satisfaction. In contrast, a former marriage and a never marriage were associated with lower life satisfaction. Migration status was also significantly associated with life satisfaction, where women who changed their location were more satisfied than those who never changed their current location. Bangladesh aims to achieve the Sustainable Development Goals (SDGs) by 2030. To achieve the Sustainable Development Goal (SDG) 3, which promotes well-being, and the goal 5, to empower all women and girls, addressing the issues related to life satisfaction and applying appropriate intervention is a must.

Recognizing cholera cases early, especially in the initial phase of an outbreak and in areas where cholera has not previously circulated, is a high public health priority. Laboratory capacity in such settings is often limited. To address this, we have developed a rapid diagnostic test (RDT) termed Cholkit that is based on an immunochromatographic lateral flow assay for the diagnosis of cholera cases using stool. Cholkit contains a monoclonal antibody (ICL-33) to the O-specific polysaccharide (OSP) component of V. cholerae O1 lipopolysaccharide, and recognizes both Inaba and Ogawa serotypes. We tested the Cholkit dipstick using fresh stool specimens of 76 adults and children presenting with acute watery diarrhea at the icddr,b hospital in Dhaka, Bangladesh. We compared Cholkit's performance with those of microbial culture, PCR (targeting the rfb and ctxA genes of V. cholerae) and the commercially available RDT, Crystal VC (Span Diagnostics; Surat, India). We found that all stool specimens with a positive culture for V. cholerae O1 (n = 19) were positive by Cholkit as well as Crystal VC. We then used Bayesian latent class modeling to estimate the sensitivity and specificity of each diagnostic assay. The sensitivity of Cholkit, microbiological culture, PCR and Crystal VC was 98% (95% CI: 88-100), 71% (95% CI: 59-81), 74% (95% CI: 59-86) and 98% (95% CI: 88-100), respectively. The specificity for V. cholerae O1 was 97% (95% CI: 89-100), 100%, 97% (95% CI: 93-99) and 98% (95% CI: 92-100), respectively. Of note, two Crystal VC dipsticks were positive for V. cholerae O139 but negative by culture and PCR in this area without known circulating epidemic V. cholerae O139. In conclusion, the Cholkit dipstick is simple to use, requires no dedicated laboratory capacity, and has a sensitivity and specificity for V. cholerae O1 of 98% and 97%, respectively. Cholkit warrants further evaluation in other settings.

Cholera rapid diagnostic tests (RDTs) are vulnerable to virulent bacteriophage predation. We hypothesized that an enhanced cholera RDT that detects the common virulent bacteriophage ICP1 might serve as a proxy for pathogen detection. We previously developed a monoclonal antibody (mAb) to the ICP1 major capsid protein. Our objective herein was to design and assemble a first-of-its-kind RDT that detects both a bacterial pathogen (Vibrio cholerae) and associated virulent bacteriophage (ICP1). Candidate mAbs were expanded to increase design options and evaluated by immunological assays (ELISA; western blot). A subset of mAbs were selected for gold conjugation and printing on the RDT. The limit of detection (LOD) of prototype RDTs were determined in diarrheal stools with the addition of ICP1. Three mAb candidates were developed and evaluated for the capsid decoration protein (ORF123) and tail fiber protein (ORF93), and the prior mAb for the major capsid protein (ORF122). A single mAb sandwich RDT prototype for ORF122 was able to detect ICP1; RDTs with mAbs to ORF123 and ORF93 failed to detect ICP1 in single or dual sandwich configurations. Biologically meaningful LODs for ICP1 were achieved only after boiling the stool with ICP1; analysis by electron microscopy suggested increased epitope availability after boiling. In this study, we demonstrate a proof of concept for a functional RDT that can detect both the primary pathogen and a common virulent bacteriophage as a proxy for pathogen detection. Further optimization is required before scaled production and implementation.

Background The 15q11–q13 region is a genetic locus with genes subject to genomic imprinting, significantly influencing neurodevelopment. Genomic imprinting is an epigenetic phenomenon that causes differential gene expression based on the parent of origin. In most diploid organisms, gene expression typically involves an equal contribution from both maternal and paternal alleles, shaping the phenotype. Nevertheless, in mammals, including humans, mice, and marsupials, the functional equivalence of parental alleles is not universally maintained. Notably, during male and female gametogenesis, parental alleles may undergo differential marking or imprinting, thereby modifying gene expression without altering the underlying DNA sequence. Neurodevelopmental disorders, such as Prader–Willi syndrome (PWS) (resulting from the absence of paternally expressed genes in this region), Angelman syndrome (AS) (associated with the absence of the maternally expressed UBE3A gene), and 15q11–q13 duplication syndrome (resulting from the two common forms of duplications—either an extra isodicentric 15 chromosome or an interstitial 15 duplication), are the outcomes of genetic variations in this imprinting region. Methods Conducted a genomic study to identify the frequency of pathogenic variants impacting the 15q11–q13 region in an ethnically homogenous population from Bangladesh. Screened all known disorders from the DECIPHER database and identified variant enrichment within this cohort. Using the Horizon analysis platform, performed enrichment analysis, requiring at least >60% overlap between a copy number variation and a disorder breakpoint. Deep clinical phenotyping was carried out through multiple examination sessions to evaluate a range of clinical symptoms. Results This study included eight individuals with clinically suspected PWS/AS, all previously confirmed through chromosomal microarray analysis, which revealed chromosomal breakpoints within the 15q11–q13 region. Among this cohort, six cases (75%) exhibited variable lengths of deletions, whereas two cases (25%) showed duplications. These included one type 2 duplication, one larger atypical duplication, one shorter type 2 deletion, one larger type 1 deletion, and four cases with atypical deletions. Furthermore, thorough clinical assessments led to the diagnosis of four PWS patients, two AS patients, and two individuals with 15q11–q13 duplication syndrome. Conclusion Our deep phenotypic observations identified a spectrum of clinical features that overlap and are unique to PWS, AS, and Dup15q syndromes. Our findings establish genotype–phenotype correlation for patients impacted by variable structural variations within the 15q11–q13 region.   

Background The unpredicted pandemic disease COVID-19 first flared up adversely in Europe by imparting interminable force of infected and fatality cases to Italy. In late February 2020, the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in northern Italy and swiftly proliferated to the entire country, albeit continuous to date (23 March 2022) with a lesser extent of deadliness. Current review focused on the invasions and the associated consequences by SARS-CoV-2 during the period of March 2020–March 2022. Main body of the abstract Initially, the lethality and transmissibility of the novel virus made Italy stunned within 1 month, the number of death cases reached 12,428 at the end of March 2020. The Italian Government announced an immediate emergency phase in entire country, educational institutions to local businesses, manufacturing works, cultural activities to elective activities were rescinded and all the hospitals to morgues were swamped, ensuing that fear of epidemic was impended. Besides, the Italian National Health System and Service coordinated massive public health interventions and conferred unprecedented efforts to limit the high mortality rate of the first wave of infection. Amidst 2 years of epidemic (as of 23 March 2022), Italy has documented 14,070,450 (23.74% of the population) confirmed infected cases, 12,685,306 (21.41% of the population) healed cases, 158,254 death cases (0.27% of the population) and ranking 9th worldwide in the number of deaths. Short conclusion Based on publicly available Italian Ministry of Health COVID-19 data, current review has comprehended region-wise total infected cases, death cases and healed cases for three consecutive years 2020–2022 to foresee different patterns of the regional outbreak and gradual subservience. At a glance, we highlighted the overview of the exhaustion and exertion of COVID-19 crisis throughout the periods in Italy.

Introduction This study examined the mental health consequences of Bangladesh's July Revolution (2024) by assessing the occurrence of depression and post-traumatic stress disorder (PTSD) symptoms among survivors of state violence and persecution while identifying associated risk factors. Materials and methods A hospital-based cross-sectional study was conducted from September 2024 to February 2025 among 217 injured survivors (mean age: 26.0 ± 9.7 years; 97.2% male). Participants were assessed via face-to-face interviews using validated tools: Patient Health Questionnaire-9 (PHQ-9) (cutoff: ≥10 for depression) and PTSD Checklist for DSM-5 (PCL-5) (cutoff: ≥33 for PTSD). Univariate logistic regression was performed to analyze sociodemographic and clinical associations. Results Depression and PTSD were alarmingly high: 82.5% met the threshold for depression (PHQ-9 mean: 14.7 ± 5.6) and 64.1% for PTSD (PCL-5 mean: 37.6 ± 16.1). Urban survivors had significantly lower odds of depression (OR = 0.47, 95% CI: 0.22-1.00) and PTSD (OR = 0.52, 95% CI: 0.29-0.92) than rural counterparts. Strikingly, 99.3% of PTSD cases had comorbid depression, with strong symptom correlation (r = 0.772, p < 0.001). No other sociodemographic factors (age, sex, education, occupation, and socioeconomic status) or violence type showed significant associations. Conclusions The revolution's survivors exhibited extreme mental health burdens, underscoring an urgent need for trauma-integrated care, especially in rural areas.