Explore the vast range of titles available.

Radiobiological investigations on the reproductive behavior of female Spodoptera litura (Fabr.) were conducted with the aim of determining the suitable radio-sterilizing dose for females in order to release them along with sub-sterile males for effective implementation of the Inherited Sterility technique against this pest. Calling and copulation duration significantly increased, while mating success, oviposition, fertility and longevity significantly decreased with increasing radiation dose (100–200 Gy) compared to control. In view of the effect of irradiation on mating behavior and reproductive viability of female S. litura, 130 Gy was identified as a suitable radio-sterilization dose. Further molecular studies were conducted to corroborate this dose for female sterilization, along with a higher dose of 200 Gy in order to validate the gradational response of ionizing radiation. GC-MS analysis indicated decreased sex pheromone titer at 130 Gy, which was more pronounced at 200 Gy. Pheromone-associated genes, PBAN and PBAN-R showed decreased expression at 130 Gy, and were drastically reduced at 200 Gy. The fertility-related Vg gene also showed a negative correlation with radiation exposure. Based on these radiation responses of female S. litura, 130 Gy might be considered a suitable dose for complete female sterility and its inclusion in sterile insect programs against S. litura.

Mass rearing of insects of high biological quality is a crucial attribute for the successful implementation of sterile insect release programs. Various ontogenetic stages of Spodoptera litura (Fabr.) were treated with a range of low doses of ionizing radiation (0.25–1.25 Gy) to assess whether these gamma doses could elicit a stimulating effect on the growth and viability of developing moths. Doses in the range of 0.75 Gy to 1.0 Gy administered to eggs positively influenced pupal weight, adult emergence, and growth index, with a faster developmental period. The enhanced longevity of adults derived from eggs treated with 0.75 Gy and 1.0 Gy, and for larvae and pupae treated with 1.0 Gy, indicated a hormetic effect on these life stages. Furthermore, the use of these hormetic doses upregulated the relative mRNA expression of genes associated with longevity (foxo, sirtuin 2 like/sirt1, atg8) and viability/antioxidative function (cat and sod), suggesting a positive hormetic effect at the transcriptional level. These results indicated the potential use of low dose irradiation (0.75–1 Gy) on preimaginal stages as hormetic doses to improve the quality of the reared moths. This might increase the efficiency of the inherited sterility technique for the management of these lepidopteran pests.

The ‘Inherited or F1 sterility technique’ (IS), using sub-sterilized male moths, is a widely proposed pest management tool for Lepidoptera pests in general, and the tobacco cutworm Spodoptera litura (Fabr.) in particular. However, the multiple mating tendency of female moths and the ejaculate quality of male moths might influence the efficiency of this technique. Reduced ejaculate quality was observed in irradiated males, as evidenced by radiation’s impact on certain bio-parameters, such as the weight of the spermatophores and their protein content, sperm count, the molecular expression of the sex peptide receptor (SPR) and egg fertility, with a greater impact in F1 male progeny. During the remating of females with untreated males, irrespective of the irradiation status of the first male, there was an increase in calling behavior, remating propensity and fertility in females, with a larger time gap between consecutive matings. The ability of F1 male progeny to check remating propensity in females 24 h after the initial mating was lower than that of unirradiated males. Partially sterile (130 Gy) males were as successful as unirradiated males in inducing the level of mating refractoriness in females. Decreased ejaculate quality in F1 male progeny could be associated with increased female receptivity during remating. Understanding the influence of male moth irradiation, insemination quality and post (initial)-mating intervals on the remating behavior of normal female moths and induced sterility might help in simulation modeling and optimizing IS insect programs.

This paper investigates the Buchen tradition of oral narration and enactment of life stories associated with popular Tibetan Buddhist personalities and the ways in which gender and sexuality are embedded in such performances. As an exclusively male tradition, the Buchen and their troupe perform the parts of both male and female characters in rituals and in their dramatization of edifying religious stories. An accidental discovery of a video clip displaying a group of women impersonating the Buchen in Mane village, Spiti, provides an opportunity to study how certain characteristics are marked by gender roles in this society and what the implications of cross-dressing entail for both categories. An interdisciplinary approach has been employed to understand this representation of gender in the larger context of Tibetan Buddhist philosophy, and culture.

Kyrgyzstan is one of the 27 high multidrug-resistant tuberculosis (MDR-TB) burden countries listed by the WHO. In 2012, Médecins Sans Frontières (MSF) started a drug-resistant tuberculosis (DR-TB) project in Kara Suu District. A qualitative study was undertaken to understand the perception of TB and DR-TB in order to improve the effectiveness and acceptance of the MSF intervention and to support advocacy strategies for an ambulatory model of care. This paper reports findings from 63 interviews with patients, caregivers, health care providers and members of communities. Data was analysed using a qualitative content analysis. Validation was ensured by triangulation and a 'thick' description of the research context, and by presenting deviant cases. Findings show that the general population interprets TB as the 'lungs having a cold' or as a 'family disease' rather than as an infectious illness. From their perspective, individuals facing poor living conditions are more likely to get TB than wealthier people. Vulnerable groups such as drug and alcohol users, homeless persons, ethnic minorities and young women face barriers in accessing health care. As also reported in other publications, TB is highly stigmatised and possible side effects of the long treatment course are seen as unbearable; therefore, people only turn to public health care quite late. Most patients prefer ambulatory treatment because of the much needed emotional support from their social environment, which positively impacts treatment concordance. Health care providers favour inpatient treatment only for a better monitoring of side effects. Health staff increasingly acknowledges the central role they play in supporting DR-TB patients, and the importance of assuming a more empathic attitude. Health promotion activities should aim at improving knowledge on TB and DR-TB, reducing stigma, and fostering the inclusion of vulnerable populations. Health seeking delays and adherence problems will be countered by further implementation of shortened treatment regimens. An ambulatory model of care is proposed when convenient for the patient; hospitalisation is favoured only when seen as more appropriate for the respective individual.

The 'Inherited or F sterility technique' (IS), using sub-sterilized male moths, is a widely proposed pest management tool for Lepidoptera pests in general, and the tobacco cutworm (Fabr.) in particular. However, the multiple mating tendency of female moths and the ejaculate quality of male moths might influence the efficiency of this technique. Reduced ejaculate quality was observed in irradiated males, as evidenced by radiation's impact on certain bio-parameters, such as the weight of the spermatophores and their protein content, sperm count, the molecular expression of the sex peptide receptor ( ) and egg fertility, with a greater impact in F male progeny. During the remating of females with untreated males, irrespective of the irradiation status of the first male, there was an increase in calling behavior, remating propensity and fertility in females, with a larger time gap between consecutive matings. The ability of F male progeny to check remating propensity in females 24 h after the initial mating was lower than that of unirradiated males. Partially sterile (130 Gy) males were as successful as unirradiated males in inducing the level of mating refractoriness in females. Decreased ejaculate quality in F male progeny could be associated with increased female receptivity during remating. Understanding the influence of male moth irradiation, insemination quality and post (initial)-mating intervals on the remating behavior of normal female moths and induced sterility might help in simulation modeling and optimizing IS insect programs.

Spodoptera litura (Fabr.), an important pest of agricultural crops in India, can be controlled using the ‘Inherited or F[sub.1] sterility technique’. The multiple mating tendency in female moths, their receptivity to remating after initial mating with radio-sterilized males and the sperm use pattern in sequential matings might influence the level of sterility induced in a radiation-mediated F[sub.1] sterility program. In this study, the effect of irradiation was assessed on the quality of the ejaculate of exposed male moths, with a substantial decrease in insemination quality in their F[sub.1] male progeny. Females mated to F[sub.1] males showed an increased calling efficiency and remating propensity (with normal males) and influenced reproductive performance with a prolonged post (initial)-mating interval compared with the control moths. These findings on factors influencing female receptivity during remating and eventual reproductive sterilization might help optimize the technique. The ‘Inherited or F[sub.1] sterility technique’ (IS), using sub-sterilized male moths, is a widely proposed pest management tool for Lepidoptera pests in general, and the tobacco cutworm Spodoptera litura (Fabr.) in particular. However, the multiple mating tendency of female moths and the ejaculate quality of male moths might influence the efficiency of this technique. Reduced ejaculate quality was observed in irradiated males, as evidenced by radiation’s impact on certain bio-parameters, such as the weight of the spermatophores and their protein content, sperm count, the molecular expression of the sex peptide receptor (SPR) and egg fertility, with a greater impact in F[sub.1] male progeny. During the remating of females with untreated males, irrespective of the irradiation status of the first male, there was an increase in calling behavior, remating propensity and fertility in females, with a larger time gap between consecutive matings. The ability of F[sub.1] male progeny to check remating propensity in females 24 h after the initial mating was lower than that of unirradiated males. Partially sterile (130 Gy) males were as successful as unirradiated males in inducing the level of mating refractoriness in females. Decreased ejaculate quality in F[sub.1] male progeny could be associated with increased female receptivity during remating. Understanding the influence of male moth irradiation, insemination quality and post (initial)-mating intervals on the remating behavior of normal female moths and induced sterility might help in simulation modeling and optimizing IS insect programs.

Spodoptera litura (Fabr.) is a serious noctuid lepidopteran pest in India that can be managed using the eco-friendly, radio-genetic inherited sterility (IS) technique. Improving the quality of laboratory-reared moths might increase the efficacy of released sterile moths using this technique. In the present study, radiation hormesis using low-dose ionizing radiation administered to different ontogenetic stages was studied. The growth potential and survival of the developing stages derived from these treated stages was assessed, followed by an assessment of the expression profile of longevity- and viability-related genes. The findings indicated that ionizing radiation doses of 0.75–1.0 Gy might be used as hormetic treatments in eggs, larvae, or the pupal stages of S. litura. This would result in more viable and competitive adult moths for use in the IS technique. Mass rearing of insects of high biological quality is a crucial attribute for the successful implementation of sterile insect release programs. Various ontogenetic stages of Spodoptera litura (Fabr.) were treated with a range of low doses of ionizing radiation (0.25–1.25 Gy) to assess whether these gamma doses could elicit a stimulating effect on the growth and viability of developing moths. Doses in the range of 0.75 Gy to 1.0 Gy administered to eggs positively influenced pupal weight, adult emergence, and growth index, with a faster developmental period. The enhanced longevity of adults derived from eggs treated with 0.75 Gy and 1.0 Gy, and for larvae and pupae treated with 1.0 Gy, indicated a hormetic effect on these life stages. Furthermore, the use of these hormetic doses upregulated the relative mRNA expression of genes associated with longevity (foxo, sirtuin 2 like/sirt1, atg8) and viability/antioxidative function (cat and sod), suggesting a positive hormetic effect at the transcriptional level. These results indicated the potential use of low dose irradiation (0.75–1 Gy) on preimaginal stages as hormetic doses to improve the quality of the reared moths. This might increase the efficiency of the inherited sterility technique for the management of these lepidopteran pests.

Spodoptera litura (Fabr.) is a serious Lepidopteran pest that can be controlled using the eco-friendly radio-genetic Inherited (F1) Sterility (IS) technique. In contrast with the conventional male-only release applied in IS techniques, this study was conducted to determine a suitable dose at which females can be fully radio-sterilized while leaving the more radio-resistant males partially sterile, meaning that both sexes could be irradiated together and released simultaneously in pest infested fields. Behavioral and molecular investigations ascertained 130 Gy to be a suitable dose for female sterilization, at which point their reproductive fitness in terms of calling ability and mating competence will not be significantly compromised. These irradiated females would be sufficiently viable to compete with wild females for mating with wild males, ultimately suppressing the pest population. The simultaneous release of sub-sterile male and sterile female moths using the IS technique might improve its efficacy, leading to more effective pest suppression. Radiobiological investigations on the reproductive behavior of female Spodoptera litura (Fabr.) were conducted with the aim of determining the suitable radio-sterilizing dose for females in order to release them along with sub-sterile males for effective implementation of the Inherited Sterility technique against this pest. Calling and copulation duration significantly increased, while mating success, oviposition, fertility and longevity significantly decreased with increasing radiation dose (100–200 Gy) compared to control. In view of the effect of irradiation on mating behavior and reproductive viability of female S. litura, 130 Gy was identified as a suitable radio-sterilization dose. Further molecular studies were conducted to corroborate this dose for female sterilization, along with a higher dose of 200 Gy in order to validate the gradational response of ionizing radiation. GC-MS analysis indicated decreased sex pheromone titer at 130 Gy, which was more pronounced at 200 Gy. Pheromone-associated genes, PBAN and PBAN-R showed decreased expression at 130 Gy, and were drastically reduced at 200 Gy. The fertility-related Vg gene also showed a negative correlation with radiation exposure. Based on these radiation responses of female S. litura, 130 Gy might be considered a suitable dose for complete female sterility and its inclusion in sterile insect programs against S. litura.

Objectives Kyrgyzstan is one of the 27 high multidrug-resistant tuberculosis (MDR-TB) burden countries listed by the WHO. In 2012, Medecins Sans Frontieres (MSF) started a drug-resistant tuberculosis (DR-TB) project in Kara Suu District. A qualitative study was undertaken to understand the perception of TB and DR-TB in order to improve the effectiveness and acceptance of the MSF intervention and to support advocacy strategies for an ambulatory model of care. Methods This paper reports findings from 63 interviews with patients, caregivers, health care providers and members of communities. Data was analysed using a qualitative content analysis. Validation was ensured by triangulation and a 'thick' description of the research context, and by presenting deviant cases. Results Findings show that the general population interprets TB as the 'lungs having a cold' or as a 'family disease' rather than as an infectious illness. From their perspective, individuals facing poor living conditions are more likely to get TB than wealthier people. Vulnerable groups such as drug and alcohol users, homeless persons, ethnic minorities and young women face barriers in accessing health care. As also reported in other publications, TB is highly stigmatised and possible side effects of the long treatment course are seen as unbearable; therefore, people only turn to public health care quite late. Most patients prefer ambulatory treatment because of the much needed emotional support from their social environment, which positively impacts treatment concordance. Health care providers favour inpatient treatment only for a better monitoring of side effects. Health staff increasingly acknowledges the central role they play in supporting DR-TB patients, and the importance of assuming a more empathic attitude. Conclusions Health promotion activities should aim at improving knowledge on TB and DR-TB, reducing stigma, and fostering the inclusion of vulnerable populations. Health seeking delays and adherence problems will be countered by further implementation of shortened treatment regimens. An ambulatory model of care is proposed when convenient for the patient; hospitalisation is favoured only when seen as more appropriate for the respective individual.