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Pregnant women undergo a myriad of physiological changes, including important hormonal variations. Pregnancy gingivitis is a condition that affects up to 30% to 100% of women, is related to hormonal modifications, and could play an important role in gestational gut colonization and immunological training of the newborn. Nonetheless, oral health is not always included in routine prenatal care. In this study, we collected saliva samples of pregnant women with and without pregnancy gingivitis and analyzed the oral microbiota through 16S sequencing. In addition, meconium samples from the infants of participating women were analyzed. The oral microbiota of pregnant women with and without pregnancy gingivitis did not show significant differences in diversity. However, significant differences in microbiome composition were observed. Pathway analysis showed that, despite taxonomic similarity, the PG group had activated energy metabolism, bacterial growth, lipid metabolism, and virulence pathways with NOD-like receptor activation, indicating pro-inflammatory microbial activity. In contrast, the NPG group exhibited central metabolism and repair mechanisms, suggesting that PG could affect microbiome function rather than composition. In addition, it appears that the microbiome composition of offspring of mothers with gingivitis also differs from that of offspring from mothers without gingivitis, although the number of available samples did not allow for definite conclusions. As such, a larger cohort and deeper sequencing methods are needed to assess the oral microbiota of pregnant women with and without gingivitis and to explore the possibility of bacterial translocation from the maternal gingiva to the fetal gut.

The oral microbiome of patients with acute lymphoblastic leukemia (ALL) undergoes changes caused by the neoplasia as well as the antimicrobial activity of chemotherapy (CTX), which promotes the development of oral mucositis (OM). This study aimed to analyze the oral microbiome dynamics and salivary cytokine production in pediatric ALL patients before and during CTX, comparing children who did and did not develop OM. We conducted a longitudinal, observational, and analytical study including 32 newly diagnosed pediatric ALL patients (ages 2–16 years) undergoing CTX. Oral rinse and non-stimulated saliva samples were collected at baseline (day 0), day 14, and day 21 of induction of CTX, with an additional sample taken during OM episodes when possible. Microbiome analysis was performed using 16S rRNA sequencing on an Illumina MiSeq platform, and salivary cytokines were measured using a Luminex multiplex assay. The most pronounced microbiome changes occurred on day 14, particularly in patients who developed OM, characterized by higher α diversity, increased abundance of opportunistic taxa, and elevated IL-6 concentrations. In contrast, patients who did not develop OM exhibited a more stable microbial composition. Overall, these findings indicate that temporal oral dysbiosis and increased IL-6 may serve as early markers and potential predictors of OM development during chemotherapy in pediatric ALL patients.

This article provides the foundation for employing nonparametric testing in dental clinical research. To make wise judgments in their research, investigators should learn more about the main nonparametric tests and their particular uses. Biostatistical analysis is essential in dental research; dental research frequently deviates from the assumptions that underpin traditional parametric statistics. Nonparametric statistics are useful for studies with small sample sizes, nominal- or ordinal-level data, and non-normally distributed variables. These statistical tests make no assumptions about the sampled population. Nonparametric tests are statistical methods based on signs and ranks. For dental research to be conducted effectively and accurately, statistical approaches must be applied correctly. Therefore, dental researchers must understand the many statistical methods at their disposal and know when to use them.

Background/Objectives: Children with Autism Spectrum Disorder (ASD) often exhibit similar food-related behaviors, such as excessive sugar consumption, and sensory processing difficulties, which can hinder oral hygiene routines like toothbrushing and increase the risk of cavities or gum problems. Therefore, caregiver involvement in maintaining oral health is crucial. The aim of this study was to assess the knowledge, attitudes, and practices reported by caregivers in terms of oral care provided to children diagnosed with ASD between the ages of 5 and 12 years. Methods: A cross-sectional study was conducted, and the participants comprised 72 caregivers of children with ASD enrolled in four therapeutic centers in SLP, Mexico. Data on caregivers’ knowledge, attitudes, and care in terms of oral health, as well as sociodemographic characteristics, were collected through a structured and self-administered questionnaire. Results: Among the caregivers, 85% were women, and 86% recognized sugar as a cariogenic and gingival bleeding as a sign of inflammation. Despite this, over 60% reported frequent sugar consumption, 65.4% supervised toothbrushing, and floss use was minimal. More than half showed high self-efficacy, which correlated with more frequent supervised or autonomous toothbrushing. Caregivers involved in homecare brushed their children’s teeth more often. Correct knowledge of brushing frequency was associated with actual toothbrushing practices (p < 0.05). Conclusions: This study highlights a notable gap between caregivers’ knowledge and oral care practices in terms of children with ASD.

Bacteriotherapy is a promising option in addressing dental caries, a persistent global public health challenge with multifactorial origin, including dysbiosis. Despite the exploration of various probiotics, outcomes remain inconclusive. Objective: This study aimed to assess the inhibitory potential of L. reuteri and other potential probiotics like S. salivarius and S. oralis on the growth, adhesion, colonization, and viability of major cariogenic pathogens, comparing their probiotic efficacy. Methods: An in vitro experimental study was conducted, encompassing direct competition assays in solid and liquid co-culture tests and the characterization of adhesion to dental enamel and cell viability by life or death assay. Results: L. reuteri exhibited the significant inhibition of S. sobrinus and S. mutans growth in both solid and liquid cultures, with statistically notable differences. Scanning electron microscopy and confocal microscopy demonstrated reduced cariogenic biofilm formation when combined with L. reuteri, corroborated by diminished bacterial viability and decreased dental enamel coverage. These findings underscore L. reuteri’s potential as an effective agent in caries prevention. Conclusion: The study suggests L. reuteri could serve as an effective probiotic in bacteriotherapy against dental caries. It displayed substantial inhibitory activity in vitro against cariogenic bacteria, impeding biofilm formation and adhesion, thereby impacting cell viability.

Odontogenic tumors constitute a group of heterogeneous lesions of benign and malignant neoplasms with variable aggressiveness. Ameloblastomas are a group of benign but locally invasive neoplasms that occur in the jaws and are derived from epithelial elements of the tooth-forming apparatus. We previously described orosomucoid-1 protein expression in odontogenic myxomas. However, whether orosomucoid-1 is expressed in other odontogenic tumors remains unknown. Since orosomucoid-1 belongs to a group of acute-phase proteins and has many functions in health and disease, we identified and analyzed orosomucoid-1 expression in ameloblastoma variants and ameloblastic carcinoma using western blot and immunohistochemical techniques. Thirty cases of ameloblastoma were analyzed for orsomucoid-1; five specimens were fresh for western blot study (four benign ameloblastomas and one ameloblastic carcinoma), and 25 cases of benign ameloblastoma for immunohistochemical assays. Orosomucoid-1 was widely expressed in each tumor variant analyzed in this study, and differential orosomucoid-1 expression was observed between benign and malignant tumor. Orosomucoid-1 may play an important role in the behavior of ameloblastomas and influence the biology and development of the variants of this tumor.

Pediatric oral oncology represents a complex and challenging field within pediatric dentistry and oncology. This brief review aims to examine current understanding, diagnostic approaches, and treatment modalities for oral and maxillofacial malignancies in the pediatric population. Special emphasis is placed on early detection, multidisciplinary treatment strategies, and management of treatment-related complications. Recent advances in targeted therapies and their impact on survival rates are also discussed. La oncología oral pediátrica representa un campo complejo y desafiante dentro de la odontología y la oncología pediátricas. Esta breve revisión tiene como objetivo examinar la comprensión actual, los enfoques diagnósticos y las modalidades de tratamiento para las neoplasias malignas orales y maxilofaciales en la población pediátrica. Se hace especial hincapié en la detección precoz, las estrategias de tratamiento multidisciplinario y el tratamiento de las complicaciones relacionadas con el tratamiento. También se discuten los avances recientes en terapias dirigidas y su impacto en las tasas de supervivencia.

Pediatric oral oncology represents a complex and challenging field within pediatric dentistry and oncology. This brief review aims to examine current understanding, diagnostic approaches, and treatment modalities for oral and maxillofacial malignancies in the pediatric population. Special emphasis is placed on early detection, multidisciplinary treatment strategies, and management of treatment-related complications. Recent advances in targeted therapies and their impact on survival rates are also discussed. La oncología oral pediátrica representa un campo complejo y desafiante dentro de la odontología y la oncología pediátricas. Esta breve revisión tiene como objetivo examinar la comprensión actual, los enfoques diagnósticos y las modalidades de tratamiento para las neoplasias malignas orales y maxilofaciales en la población pediátrica. Se hace especial hincapié en la detección precoz, las estrategias de tratamiento multidisciplinario y el tratamiento de las complicaciones relacionadas con el tratamiento. También se discuten los avances recientes en terapias dirigidas y su impacto en las tasas de supervivencia.

The global burden of pediatric severe respiratory illness is substantial, and influenza viruses contribute to this burden. Systematic surveillance and testing for influenza among hospitalized children has expanded globally over the past decade. However, only a fraction of the data has been used to estimate influenza burden. In this analysis, we use surveillance data to provide an estimate of influenza-associated hospitalizations among children worldwide. We aggregated data from a systematic review (n = 108) and surveillance platforms (n = 37) to calculate a pooled estimate of the proportion of samples collected from children hospitalized with respiratory illnesses and positive for influenza by age group (<6 mo, <1 y, <2 y, <5 y, 5-17 y, and <18 y). We applied this proportion to global estimates of acute lower respiratory infection hospitalizations among children aged <1 y and <5 y, to obtain the number and per capita rate of influenza-associated hospitalizations by geographic region and socio-economic status. Influenza was associated with 10% (95% CI 8%-11%) of respiratory hospitalizations in children <18 y worldwide, ranging from 5% (95% CI 3%-7%) among children <6 mo to 16% (95% CI 14%-20%) among children 5-17 y. On average, we estimated that influenza results in approximately 374,000 (95% CI 264,000 to 539,000) hospitalizations in children <1 y-of which 228,000 (95% CI 150,000 to 344,000) occur in children <6 mo-and 870,000 (95% CI 610,000 to 1,237,000) hospitalizations in children <5 y annually. Influenza-associated hospitalization rates were more than three times higher in developing countries than in industrialized countries (150/100,000 children/year versus 48/100,000). However, differences in hospitalization practices between settings are an important limitation in interpreting these findings. Influenza is an important contributor to respiratory hospitalizations among young children worldwide. Increasing influenza vaccination coverage among young children and pregnant women could reduce this burden and protect infants <6 mo.

To clinically and epidemiologically characterize idiopathic gingival papillokeratosis with crypt formation (IGPCF) in a cohort of patients, aiming to enhance understanding and improve diagnosis and management of this rare condition. A cross-sectional study was conducted at the Diagnostic Clinic of the School of Stomatology, UASLP, between August 2022 and April 2023, including patients with complete electronic medical records containing demographic and medical information. Clinical diagnosis was supported by photographic documentation and cytological examination to rule out other pathologies. Group comparisons were performed with chi-square or Fisher’s exact tests. Logistic regression was applied to assess associations. A total of 7,163 patients were analyzed; the overall prevalence of IGPCF was 1.01% (95% CI: 0.79-1.26). Women had nearly a threefold higher likelihood of presenting IGPCF compared with men (OR=2.99, 95% CI: 1.76-5.10; p<0.05). No significant associations were found with age, smoking, alcohol use, or diabetes. Lesions were predominantly located in the maxilla (89.7%) and bilateral in 62.8% of cases. Mild gingival inflammation was observed in most evaluated cases. Clinically, lesions appeared as asymptomatic, pale-yellow, papillary plaques along the mucogingival junction. IGPCF is a rare, typically asymptomatic gingival condition with a distinct bilateral presentation in the maxillary gingiva, mostly affecting young adults. Proper identification is essential to prevent misdiagnosis. This study contributes the largest clinical dataset to date on IGPCF, reinforcing the need for standardized diagnostic criteria to support accurate clinical recognition and effective management.