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One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of which ISO 22916 is the standard. To assess the performance of different testing methodologies to analyse the antibacterial activity of hydrophobic surface patterned plastics as part of a Horizon 2020 European research project. Four different testing methods were used to study the antibacterial activity of a patterned film, including the ISO 22916 standard, the immersion method, the touch-transfer inoculation method, and the swab inoculation method, this latter developed specifically for this project. The non-realistic test conditions of the ISO 22916 standard showed this method to be non-appropriate in the study of hydrophobic patterned surfaces. The immersion method also showed no differences between patterned films and smooth controls due to the lack of attachment of testing bacteria on both surfaces. The antibacterial activity of films could be demonstrated by the touch-transfer and the swab inoculation methods, that more precisely mimicked the way of high-touch surfaces contamination, and showed to be the best methodologies to test the antibacterial activity of patterned hydrophobic surfaces. A new ISO standard would be desirable as the reference method to study the antibacterial behaviour of patterned surfaces.

Many studies have illustrated the great benefit of ground-penetrating radar (GPR) in civil engineering. However, in some cases, this geophysical survey method does not produce the desired results due to the electromagnetic characteristics of the subsoil. This study presents the results obtained in two locations near Linares (southern Spain), evaluating the detection of structures buried in conductive host materials (0.02 S/m in site 1 and 0.015 S/m in site 2) characterized by strong signal attenuation. Accounting for the study depth, which was 1.5 m, a 500 MHz shielded GPR antenna was used at both sites. At the first site, a controlled experiment was planned, and it consisted of burying three linear elements. An iron pipe, a PVC pipe, and a series of precast blocks were buried at a depth of 0.5 m in a subsoil composed of highly conductive clayey facies. To eliminate additional multiples caused by other superficial structures and increasing the high-frequency content, the predictive deconvolution flow was applied. In the 3D processing, the cover surfaces technique was used. Once the acquired GPR signals was analyzed and the optimal processing flow established, a second site in which different infrastructures in a conductive host medium formed by marly facies was explored. The 2D flow and 3D processing applied in this work allows to detect and see the continuity of some structures not visible for the default processing.