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Anisakids are widespread marine parasites of medical, veterinary and economic relevance. They infect marine natural hosts but humans can accidentally acquire the fish-borne zoonosis anisakiasis by ingesting infected raw fishes or mollusks. Among the several species described, Anisakis pegreffii is one of the main etiological agent of the disease, in particular in the Mediterranean area. Despite the growing evidence of miRNAs involvement in host-parasite interplay, and the emerging role of exosomal microvesicles in shuttling them between different cell types (and sometime across species), no information on miRNAs from any Anisakis species is presently available. In this study we isolated extracellular vesicles (EVs) released by Anisakis pegreffii infective third-stage larvae (L3) and analyzed by RNA-seq small RNAs from both L3 and EVs. We showed by nanoparticle tracking analysis that L3 release in culture medium particles of size compatible with the one of extracellular vesicles. A catalogue of 156 miRNAs from A. pegreffii was compiled by sequence comparison to evolutionary close species and miRNA prediction software. Using differential expression analysis, we identified a small number of highly abundant miRNAs in larvae and extracellular vesicles fractions whose potential biological relevance may deserve future investigation. Finally, A. pegreffii miRNAs were compared to those described in other parasitic helminths and predicted targets among human genes were searched, suggesting their potential involvement during infection.

At a routine health check of a female peregrine falcon, 23 trematodes preliminary identified as Prosthogonimus sp. were removed from the bursa of Fabricius. Based on morphological and molecular examination, a new species, Prosthogonimus falconis, was described. The pear-shaped flukes were 4.3–6.9 mm long, with greatest width posterior to testes. Tegumental spines measuring between 17 and 21 μm long covered the whole body. Length and width ratio of oral to ventral suckers were 1:1.3. Extracaecal, multifollicular vitelline glands commenced prior to acetabulum and terminated posterior to testes. Eggs in the distal uterus measured 21 × 12 μm. Molecular analysis of internal transcribed spacer 2, cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 gene regions revealed that the new species described here is phylogenetically closest to Prosthogonimus cuneatus and Prosthogonimus pellucidus clusters.

Recent fire seasons have highlighted the growing threats posed by wildfires, despite efforts in suppression policies. These challenges arise from the complex interplay of climate change, fuel availability, land use, and socio-economic conditions. In Italy, the community of scientists, policy- and decision-makers, and practitioners is converging on the urgent need for changes in the prevailing paradigm that governs wildfire policy and management strategies, and advocate for new perspectives and a holistic approach that integrates prevention, mitigation, and response strategies. In recent years, Italy has significantly restructured its wildfire governance system, highlighting both the strengths and limitations of shifting paradigms in fire management in Europe. This paper examines the current wildfire management in Italy, providing a historical timeline of wildfire management in the country and highlighting its distinct features compared to other Mediterranean countries. Conducting an exhaustive review of recent Italian scientific research on wildfire management, this study outlines key observations that highlight areas for improvement. The paper finally aims to provide specific recommendations, presented as future policy considerations, for improving the resilience and sustainability of the Italian wildfire management system.

Fire exposure describes the spatial juxtaposition of values with fire behaviour in terms of likelihood and intensity. Wildfire exposure analysis is based on the estimation of the potential wildfire intensity and on the burn probability. Fire modelling can produce spatially explicit information on fire spread and behaviour, and offers a feasible method to simulate, map, and analyse fire exposure. FlamMap Minimum Travel Time (MTT) algorithm (Finney, 2006) was used to conduct wildfire simulations considering historical data of fuel moisture conditions and winds, as well as the most frequent wind directions and historical ignition locations (2005-2018). Analysis was conducted on spatial and quantitative variations in selected fire hazard and exposure factors, namely Burn Probability (BP), Conditional Flame Length (CFL) and Fire Size (F). We observed pronounced spatial variations among and between municipalities in the factors, especially for those in the northern and southern parts of Ardabil. The variations across the burnable area of the municipalities can be fundamentally related to a number of factors, including spatial variation in ignition locations, fuel moisture and load, weather conditions, and topography of the terrain. The findings can provide information and support in wildfire management planning and fire risk mitigation activities.

Surface biomass characterization plays a key role in wildfire management. It allows classifying vegetation fuels flammability for fire risk analysis, to define silvicultural prescriptions for fire hazard reduction, to plan prescribed burning, or to model fire behavior and its effects, such as greenhouse gas and pollutant emissions. To facilitate fuel classification and analysis of potential fire behavior and effects in Italy, we harmonized 634 measurements of surface wildland fuels from Alpine, temperate and Mediterranean environments. The dataset provides quantitative data for duff, fine dead fuels and downed woody material, live grasses and shrubs fuel components. Surface fuel data were harmonized by subdividing loads (Mg ha-1) to standard size classes for dead (0-6, 6-25 and 25-75 mm) and live (0-6, 6-25 mm) fuels, collecting percent cover and depth/height (cm) of the various fuel components, and classifying observations into 19 fuelbed categories. To ensure comparability with existing vegetation classification systems, we classified each observation according to the European Fuel Map, the Corine Land Cover classes (level IV), the European Forest Types, and the forest categories of the Italian National Forest Inventory. The dataset and a photo description of each fuelbed category are available as Supplementary material. This dataset is the first step to develop several products at the national scale such as: (i) fuel type classification and mapping; (ii) carbon stock and wildfire emission estimates; (iii) calibration of fuel models for the simulation of fire behavior and effects.

The fire danger (FD) defines the conditions less or more favourable for a fire ignition success and its propagation. FD indexes, that integrates environmental variables related to FD in more or less complex equations and systems, are widely used in wildfire prone countries for both scientific and operational purposes. Assessing the performance of FD indexes is challenging and this issue is quite debated within the fire community, which has been trying to apply several methodologies to evaluate FD indexes. The main aim of this work is to give a contribution to this effort. The analysis was conducted using data from a fire-prone Mediterranean area (Sardinia island, Italy), where 8 FD indexes were evaluated and compared using different statistical approaches. We calculated the daily FD values for the period 2000-2007 over the study area. A set of statistical tools (namely Spearman rank correlation, Index Value Distribution and Percentile Analysis, and Logistic Regression) were applied to evaluate the performance of each FD index by comparing FD values with fire occurrence indicators. The statistical tests revealed a large variability in FD indexes performance, depending also on fire activity conditions. Our results showed that two of the tested FD indexes reached a good overall performance. Findings from this study can help both the scientific community and local fire managers, supporting the evaluation of early warning systems and fire prevention strategies in the Mediterranean Basin.

Level-set approaches are efficient and versatile methods for solving interface tracking problems and have been used in recent years to describe wildland fire propagation. Being based on an Eulerian description of the spread problem, their numerical implementation offers improved computational agility and better portability to parallel computing environments with respect to vector-based simulators. The use of a continuous representation of the fire perimeter in place of the binary formulation used in Cellular Automata avoids the commonly observed distortion of the fire shape. This work presents an algorithm for fire-spread simulation based on a level-set formulation. The results are compared to the ones obtained by two well-known Cellular Automata simulators under homogeneous conditions, and to the ones given by a well-know vector-based fire-spread simulator under realistic slope and wind conditions. According to this work, the level-set approach provides better results, in terms of accuracy, at a much reduced computational cost.

The signal sequence trap method was used to isolate cDNAs corresponding to proteins containing secretory leader peptides and whose genes are expressed specifically in the salivary glands of the malaria vector Anopheles gambiae. Fifteen unique cDNA fragments, ranging in size from 150 to 550 bp, were isolated and sequenced in a first round of immunoscreening in COS-7 cells. All but one of the cDNAs contained putative signal sequences at their 5' ends, suggesting that they were likely to encode secreted or transmembrane proteins. Expression analysis by reverse transcription-PCR showed that at least six cDNA fragments were expressed specifically in the salivary glands. Fragments showing a high degree of similarity to D7 and apyrase, two salivary gland-specific genes previously found in Aedes aegypti, were identified. Of interest, three different D7-related cDNAs that are likely to represent a new gene family were found in An. gambiae. Moreover, three salivary gland-specific cDNA fragments that do not show similarity to known proteins in the databases were identified, and the corresponding full length cDNAs were cloned and sequenced. RNA in situ hybridization to whole female salivary glands showed patterns of expression that overlap only in part those observed in the culicine mosquito A. aegypti

Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly (medfly) Ceratitis capitata with a germline transformation system based on the transposable element Minos from Drosophila hydei. Transformants were identified as phenotypic revertants of a white-eyed mutation carried by the recipient strain. Clusters of transformants were detected among the progeny of 390 individuals screened for germline transformation. Five independent and phenotypically active integration events were identified, in each of which a single copy of the transposon was inserted into a different site of the medfly genome. Molecular analysis indicates that they represent transposase-mediated insertions of the transposon into medfly chromosomes.

A commercial sandwich ELISA (Platelia Aspergillus EIA; Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive falcons and 182 samples from 142 aspergillosis-negative falcons were tested. The sensitivity of the test was only 12 per cent and its specificity was 95 per cent. The test was therefore unsatisfactory for detecting galactomannan in the serum samples and cannot be used as a screening test for aspergillosis in falcons.