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The hexavalent chromium is one of the major carcinogenic components released during the tanning process and lots of work have been carried out on the reduction of hexavalent chromium via chemical and biological routes. Different fatty oils are also employed in the tanning process and have also been released as an effluent along with chromium. However, it is difficult to find a study on the reduction of chromium in the presence of other contaminant which would help to mimic the real-time complication of treating the tannery effluent. It is the first attempt on the reduction of hexavalent chromium in the presence of synthetic fatty liquor and neatsfoot oil using Fusarium oxysporum . The maximum percentage of chromium reduction was 73.62% and 60.28% in neatsfoot oil and synthetic fatty oil, respectively, for the initial chromium concentration of 25 mg/L. The biomass productivity was better with both neatsfoot oil and synthetic fatty oil, whereas the same has decreased with the presence of chromium. The reduction of chromium was found to follow the uncompetitive substrate inhibition kinetics than the general Michaelis–Menten kinetics. The kinetic parameters were calculated using particle swarm optimization algorithm, which were compared with the already reported data. The uncompetitive substrate inhibition kinetics was represented the experimental data in both the cases and the value of substrate inhibition constant was low in the case of neatsfoot oil compared with the synthetic fatty liquor.

The processing of shellfishery industrial wastes is gaining much interest in recent times due to the presence of valuable components. Chitin is one of the valuable components and is insoluble in most common solvents including water. In this study, a novel gram-positive bacterial strain capable of solubilizing chitin was screened from a prawn shell dumping yard. The chitinolytic activity of the isolated strain was observed through the zone of hydrolysis plate assay. The hyper-producing isolate was identified as Bacillus velezensis through the 16S rRNA sequencing technique. The structural and morphological characterization of raw and colloidal chitin preparation was carried out using FTIR, XRD, and SEM analysis. The residual protein and mineral content, degree of polymerization, and degree of acetylation were reported for both raw and colloidal chitin preparations. There was a linear increase in the chitinase activity with an increase in the colloidal chitin concentration. The maximum activity of chitinase was observed as 38.98 U/mL for the initial colloidal chitin concentration of 1.5%. Supplement of additional carbon sources, viz., glucose and maltose, did not improve the production of chitinase and resulted in a diauxic growth pattern. The maximum chitinase activity was observed to be 33.10 and 30.28 U/mL in the colloidal chitin–containing medium with and without glucose as a secondary carbon source, respectively. Interestingly, the addition of complex nitrogen sources has increased the production of chitinase. A 1.95- and 2.14-fold increase in the enzyme activity was observed with peptone and yeast extract, respectively. The chitinase was confirmed using SDS-PAGE, native PAGE, and zymograms. The optimum pH and temperature for chitinase enzyme activity were found to be 7.0 and 44 °C, respectively.