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Valorization of waste by composting converts organic waste into valuable organic supplements. Physicochemical characteristics of tomato waste (TW) limit the efficiency of the composting process. To overcome these challenges, different mixtures were investigated by integration of olive pumice (OP), sheep manure (SM), chicken manure (CM) , and sawdust as bulking agents for the optimi z ation of the composting process. Evaluations of the composting process and compost quality were carried out to measure the temperature profile, organic matter (OM) losses, concentrations of humic substances , and macro-nutriments. The results showed that the type and ratio of feedstock in the mixture influenced the temperature of the composting process. In mixtures with SM and OP, the temperature exceeded 55 °C for more than 2 weeks, ensuring hygienization. Additionally, phosphorus and potassium significantly increased when SM and CM w ere added to the composting mixture. The addition of OP increased the concentrations of humic acid–like carbon (C HA ) and fulvic acid–like carbon (C FA ). Higher content of humic substances was recorded when SM and OP were used as composting feedstock. Using SM in the mixture was found to be more efficient than CM and constitutes a suitable feedstock for composting of tomato waste, achieving successful co-operation of agronomic and animal farm sectors.

The environmental issues related to olive oil by-products and turkey manure are continuously increasing. This ecological hazard could be mitigated by the composting process. This study aimed to investigate the effect of the initial carbon to nitrogen ratio (C/N) and aeration rate (turning frequency (TF)) on mineral contents (P, K, Ca, and Na) and their leaching in the soil surface. Olive pomace (OP) and turkey manure (TM) were co-composted to prepare six mixtures at three levels of initial C/N (20, 22, and 28) and two levels of TF (once and twice a week). The results revealed a substantial effect of TF, twice a week, resulting in a loss of 36.5%, 36%, and 27% for K, Ca, and Na contents, respectively, whereas TF, once a week, preserves the maximum of nutrients in heaps with good compost maturity. The initial C/N of 28 had significantly reduced P and K by 14% and 13%, respectively, and had increased Ca and Na contents by 85% and 30 %, respectively. The leaching of almost all the studied minerals has been demonstrated after composting. Exchangeable cations have been leached more under heaps with higher C/N ratios with TF of once per week, whereas P leaching has been recorded at the maximum level under the heap with an initial C/N of 20 and turned twice weekly. The resulting composts have generally displayed good mineral quality.

This study evaluated the chemical properties of phosphocompost extracts and their effectiveness in inducing tomato seedlings resistance to Meloidogyne javanica. Phosphocomposts: Sugar beet phosphocompost (PC-SB: CP2), green waste phosphocompost (PC-GW: CP3), and olive mill waste phosphocompost (PC-OMW: CP4), were utilized to produce compost water extracts at concentrations of 1:5, 1:10, 1:20, and 1:100 g:mL and then applied as soil drenches for tomato seedlings one-week post-inoculation. The CP2 extract applied at a 1:5 dilution led to marked improvements in growth parameters, with plant height increasing by over 52.2%, shoot fresh biomass rising by approximately 52.44%, and shoot dry biomass showing a gain of 62.21%. Root biomass also rose by 33%. Chlorophyll a increased with CP4 at 1:5 and 1:100 (41.05% and 37.32%), chlorophyll b increased with CP3 at 1:5 and 1:10 (22.34% and 7.59%), while carotenes showed no variation. Polyphenols rose by 86.45–91.01% with CP2 from 1:5 to 1:20, and flavonoids increased by 64.90% with CP4 at 1:10. CP2 diminished the ultimate M. javanica population and reproduction factor by 171.43%, while CP4 at 1:20 decreased egg masses by 151.94%. The root gall index showed no variation. The chemical composition of phosphocomposts revealed that the strategic incorporation of diverse organic improvers (10%) in phosphocomposts yielded distinct nutrient signatures, with sugar beet waste enhancing PO43− (12.91 mg/L) and secondary macronutrients, green waste optimizing NO3− (69.91 mg/L) and SO42− (62.70 mg/L) availability, and olive mill waste producing superior micronutrient concentrations alongside dominant Ca (24.21 mg/L), K (392.50 mg/L), and P (9.17 mg/L) levels. Overall, the results underscore the potential of phosphocompost extracts as a viable, low-cost, and eco-friendly alternative to synthetic nematicides, offering a sustainable and resilient approach to M. javanica control while enhancing tomato plant growth.

Drought is a critical constraint for legume production in semi-arid regions, yet breeding for drought tolerance in faba bean through induced mutagenesis remains largely unexplored. To our knowledge, this is the first EMS-derived mutant population in faba bean specifically developed for drought tolerance, comprising 45 M[sub.2]/M[sub.3] lines derived from small-seeded cv. Zina and large-seeded cv. Aguadulce Superlonga), evaluated under two irrigation regimes—100% field capacity (well-watered control) and 40% field capacity (severe stress)—over two consecutive growing seasons in a randomized complete block design with three replications. Drought stress caused severe yield losses, reducing mean seed number per plant by 42.2% and mean seed weight per plant by 47.1%. Analysis of variance revealed highly significant effects of genotype, irrigation, and generation/year on both yield components. The non-significant genotype × irrigation interaction indicated similar proportional drought response across genotypes, while the non-significant three-way interaction suggested relatively consistent genotype rankings across generations/growing seasons. Among the ten drought tolerance indices evaluated, seed-number-based mean productivity (MPn) and stress tolerance index (STIn) were the most discriminating, whereas weight-based indices failed to differentiate genotypes due to the inherent seed-size contrast between botanical backgrounds. Dunnett’s comparisons identified genotype 23 (Zina-derived) as the top performer, significantly exceeding its parent for both MPn and STIn; genotypes 22, 24, 12, 3, and 15 similarly outperformed controls. Cluster analysis broadly distinguished three groups: a tolerant cluster dominated by Zina-derived lines, a moderately tolerant cluster (Zina wild-type), and a sensitive cluster of Aguadulce Superlonga-derived lines. These findings suggest that EMS mutagenesis generated potentially heritable and exploitable variation for drought tolerance, with selected lines representing promising candidates for further multi-environment validation.

Soil salinity poses a major challenge to the legume‐rhizobia symbiosis development, thereby affecting sustainable agriculture. Selecting NaCl‐tolerant strains and enhancing the native strains' fitness under salt stress are essential steps for the restoration of marginal areas. In this work, 49 Sinorhizobium meliloti strains, the rhizobial species forming symbiotic nitrogen‐fixing associations with alfalfa—including 21 de novo‐sequenced field isolates—were subjected to a thorough in vitro screening for salt tolerance at progressively higher NaCl concentrations. Field isolates showed genome‐based geographical clustering but contrasting salt tolerance abilities. Indeed, genome‐wide association (GWA) analysis on the strains' whole‐genome sequencing data indicated several loci associated with the variability in salt tolerance. Candidate genes were involved in various processes including cell wall organisation, LPS biosynthesis, quorum sensing, and carbohydrate transport and metabolism. The relationship with carbohydrate metabolism was further confirmed by Phenotype Microarray analysis which indicated salt‐tolerant strains having enhanced capacity in carbon source usage. These findings reveal synergistic pathways underlying salt tolerance and suggest candidate traits (e.g., quorum sensing, carbohydrate synthesis and modification) for developing bioinoculants to enhance legume performance in saline soils. Genome‐wide association analysis on 49 Sinorhizobium meliloti strains (21 de novo sequenced) reveals extensive phenotypic heterogeneity in salt tolerance, closely associated with quorum sensing, cell surface stability and adaptation. Salt‐tolerant strains exhibit a wider metabolic capacity, especially in carbon sources exploitation, underscoring their potential for adaptability in harsh conditions.

Several edible agro-industrial co-products have been studied as unconventional ingredients in ruminant diets to reduce the environmental impact of food production chains. When the chemical profile of co-products is characterised by the presence of bioactive molecules, they represent a promising tool to modulate rumen microbiota activity. In the Mediterranean area, the olive oil production chain generates olive oil pomace and olive tree leaves post-milling that are animal edible bio-waste. Two in vitro trials were carried out to investigate the effect of olive oil pomace and olive tree leaves as dietary ingredients on rumen fermentation and microbiome ecology. Two experimental diets, respectively containing olive oil pomace or olive tree leaves, and the related control diets, formulated to be isoproteic and isoenergetic, were fermented and then collected after 6h and 24h. Olive oil pomace increased the content of C18:1 c9 and C18:3 c9c12c15. Considering the microbial communities, the genera Butyrivibrio, Fibrobacter, and Pseudobutyrivibrio were less abundant, while Christensenellaceae_R-7_group, Manheimia, Uruburuella were more abundant in rumen liquor fermented with olive oil pomace. Similarly, the diet containing olive tree leaves increased the content of C18:1 c9 and C18:3 c9c12c15 and decreased the abundance of Pseudobutyrivibrio and Rikenellaceae_RC9_gut_group. Data reported in this study showed that the two by-products deriving from the olive oil production chain are effective in modulating microbial community in a selective manner.

Purpose Treated wastewaters are reused in agriculture to deal with the water deficit, especially in arid and semi-arid regions. However, they may contain contaminants such as heavy metals that can adversely affect the soil quality and life health. This work aimed to assess the degree of contamination of the soil and the turf leaves of a golf course irrigated in the long term (10 years) by treated wastewaters. Method Analysis of eight heavy metals (Cd, Co, Cr, Cu, Ni, Mn, Fe and Zn) was carried out at two sites. The first was irrigated by sprinkling (site 1), the second by a drip system (site 2). Results The results showed a generalized contamination of the soil at the two sites. Fe and Mn recorded the highest levels. Cd and Cr contents were significantly higher in the soil of site 1. However, all the heavy metals recorded values below the limits set by the FAO / WHO standards for soil. Turf leaves also exhibited high values of Fe and Mn contents in the two sites. Mn content was significantly higher in in the turf leaves from site 1. The results showed an accumulation of all the metals in turf leaves in which the Fe and Cd contents exceeded the FAO / WHO standards. Conclusion Long-term irrigation results in an accumulation of heavy metals in the soil and in turf leaves. Particular attention should be paid to Cd and Fe for the use of treated wastewater in agriculture. Drip irrigation system would minimize crop contamination.

Sustainable-forage production is globally increasing, especially in marginal areas where the edaphic conditions for plant growth are not optimal. Soil salinization influences the symbiotic interaction between alfalfa and rhizobia. The efficiency of different symbiotic pairs (Sinorhizobium meliloti—Medicago sativa) was evaluated in relation to NaCl application (100 mM) on two different alfalfa cultivars (Marina and Etrusca) and 21 S. meliloti strains isolated in Algeria. At 100 mM NaCl, it was observed that there was a higher variability of plant dry weight compared to the control. The strains able to improve plant growth at 100 mM NaCl were different and specific for each alfalfa cultivar, highlighting that (symbiont) G × (host) G interaction is magnified under stressed (saline) conditions (E). Three strains were then identified as candidate inoculants for M. sativa cv Marina and used for an in-field experiment with induced stress (no irrigation), together with S. meliloti GR4 (a highly competitive strain). In-field experiments, showed a high variability, and a significant difference of plant biomass was observed only for those inoculated with S. meliloti GR4. Obtained results suggest that multiple traits should be considered for inoculant-strain selection, and for an efficient translation from lab to field, it requires extensive comprehension of the mechanisms driving G × G × E interaction.

This study aimed to investigate the co-composting time effectiveness as well as the effect of the initial Carbon/Nitrogen ratio (C/N)i variation on the hygienization of olive pomace and turkey manure. Six different heaps, at 3 levels of (C/N)i ratios: 20, 22 and 28, were installed and monitored during 6 months and assessed at three steps: At the beginning, the end of thermophilicphase and the end of curing-phase. The microbial monitoring concerned 5 microbial pathogens contents, used as hygiene microbial indicators, namely: Sulphite-Reducing Anaerobes (SRA), Escherichia Coli (E. Coli), Total Aerobic Mesophilic Flora (TAMF), Staphylococci, and Salmonella spp. Initially, the mixtures showed high TAMF and Staphylococci loads. Meanwhile, SRA and E. coli populations were relatively low and Salmonella spp. was not detected. The microbial assessment showed a significant effect of composting time on the reduction of pathogens load, except for SRA where its population has increased significantly, while the (C/N)i had a non-significant effect on pathogen content of the end-product. The final values expressed as colony-forming unit per gram (CFU g-1), were as follow: Sulfite-reducing Anaerobes (≤3.1 x 103 CFU g-1), E. Coli germ used as an indicator of faecal contamination (<4 x 101 CFU g-1), Total aerobic mesophilic flora (≤1.4 x 106 CFU g-1), Staphylococci (<10 CFU g-1) and non-detection of Salmonella spp. Finally, the seed germination tests were carried out on three different seeds: lentils (Lens culinaris), barley (Hordeum vulgare) and durum wheat (Triticum turgidum) showed that the use of the compost extract is favourable for seed germination with germination index (GI%) values exceeding 85%. These results confirm the non-phytotoxicity and maturity of the composts.

In Morocco, greenhouse tomato production is a source of organic waste. This biomass constitutes an important source of carbon, macronutrients (NPK), and microelements (Mn, Zn, Cu, and Fe). This research aims to investigate the composting process of tomato plant residues by sheep manure integration at different proportions and its effect on organic matter mineralization and humification, nitrogen dynamics, and agronomic value of the final compost. The composting process was run in an experimental conditions using two different mixing ratios set up on volume basis: R1 (2:3 tomato plant residues “TPR” + 1:3 sheep manure “SM”) and R2 (1:3 tomato plant residues “TPR” + 2:3 sheep manure “SM”) and two controls TRP (1:1 tomato plant residues “TPR”) and SM (1:1 sheep manure). Parameters such as temperature, pH, EC, C:N ratio, mineral nitrogen, and macroelements (phosphorus and potassium) were measured for a period of 60 days. At the end of the experiment, compost humification ratio and compost quality were assessed. Results showed that when TPR is composted alone, nitrogen loss and lack of aeration were observed due to a lower C:N ratio and higher moisture content. The addition of sheep manure in treatment R2 not only resulted in reaching rapidly thermophile phase and maintaining it for longer periods, but also it reduced N loss, decreased electrical conductivity, and increased concentrations of humic acid and macroelements. The present results provide opportunities to improve composting of TPR as well as the agronomic quality of the produced compost by sheep manure integration.