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Despite transmitted drug resistance (TDR) surveillance is the critical role in HIV-1 prevention strategies, epidemiological data remain scarce for newly infected men who have sex with men (MSM) in Hebei Province, China. To address this data gap, we conducted a cross-sectional study involving 173 MSM recently infected with HIV-1. Plasma samples were subjected to HIV-1 RNA extraction, followed by amplification and sequencing of the pol gene region (PR-RT: 1.3 kb; IN: 0.8 kb). In this study, 173 sequences were successfully sequenced, and the sequencing success rate was 92.5% (173/187). Subtyping analysis revealed a predominance of CRF07_BC (36.4%, 63/173) and CRF01_AE (32.3%, 56/173), with a notable proportion of unique recombinant form (URF, 20.2%, 35/173). The overall TDR prevalence was 7.51% (13/173), categorized by drug class as follows: protease inhibitors (PIs, 2.31%, 4/173), nucleoside reverse transcriptase inhibitors (NRTIs, 1.16%, 2/173), non-nucleoside reverse transcriptase inhibitors (NNRTIs, 2.31%, 4/173), and integrase strand transfer inhibitors (INSTIs, 2.89%, 5/173). Notably, one participant exhibited concurrent resistance to NRTIs, NNRTIs, and INSTIs. While these findings suggest moderate TDR control in Hebei’s MSM population, we emphasize the necessity for sustained surveillance to dynamically assess treatment program efficacy, optimize second-line therapy regime, and guide public health interventions. This study provides the first comprehensive TDR profile for recently infected individuals in Hebei, offering critical insights for regional HIV-1 management.

Pollen records from archaeological sites provide a direct reflection of the vegetation in the immediate vicinity, enabling an accurate depiction of anthropogenic impacts on vegetation. In this study, we applied the biomization technique to fossil pollen data to reconstruct human impact on the biome at the Chengzishan archaeological site in western Liaoning, China, and hence to explore the response of temperate forest vegetation to human activities. The results indicate that the original vegetation at Chengzishan was warm temperate coniferous and broadleaved mixed forest (TEDE). The findings suggest a shift in biome dominance over time, with cool temperate steppe (STEP) replacing TEDE as the dominant biome in response to human activities. Combined with archaeobotanical records, we conclude that the observed vegetation changes in the pollen record were closely linked to deforestation, fire use, and agricultural activities.

We conducted an investigation of blood management in which blood transfusion recipients underwent molecular biological analysis, to trace the possible source of HIV infection. Epidemiological investigation was carried out among HIV-infected individuals. Blood transfusion recipients infected with HIV were tracked for the date of transfusion, reason for transfusion, hospital where transfusion was received, source of blood, components of transfusion, number of transfusions, and transfusion volume. A total of 285 blood transfusion recipients infected with HIV-1 were detected in Hebei over the study period, with 42.81% (122/285) detected through clinical diagnostic testing. These cases showed a concentrated distribution in southern Hebei, with local outbreak characteristics. A census of the population in Shahe County, which had a high concentration of cases, revealed that recipients of blood transfusions had an HIV infection rate of 15.54% (92/592). Post-transfusion infection frequently occurred among blood transfusion recipients at township medical institutions, with a peak in 1995. Owing to late detection of HIV infection among blood transfusion recipients, the rates of spousal transmission and mother-to-child transmission reached 20.87% and 28.05%, respectively. Around 1995, community medical institutions did not screen for HIV antibodies among paid blood donors, which was an important cause of the outbreak of HIV-1 infection among blood transfusion recipients. Our findings indicate that cases of blood transfusion-related infection decreased rapidly with gradual improvement in the HIV screening system for blood donors that began in 1995, particularly after full implementation of HIV nucleic acid testing of volunteer blood donors was begun in 2015.

Background There has been a clear increase in HIV-1 infection cases in recent years in Hebei Province, China, and transmission via blood is one of the risk factors in the early. This article aimed to investigate the HIV infection rate and control efficiency among the paid blood donor population over a period of 18 years. Methods From 1995–2013, HIV/AIDS cases among former blood donors in Hebei Province were registered and closely monitored to collect data of all-cause mortality, intervention measures to prevent family transmission, disease transmission between couples as well as between mothers and infants, and HAART therapy outcomes. Results A total of 326 cases were identified as directly infected with HIV/AIDS during plasma donation in Hebei Province. Of these, 146 cases (44.8 %) were identified in the same year as infection; 180 cases (55.2 %) were identified 1–18 years after infection because they did not participate in the 1995 screening. The final case was identified in February 2012. By 2013, the mortality rate and survival rate of plasma donor-related HIV/AIDS was 54.9 % and 45.1 %, respectively. The identified transmission rate between couples was 11.3 % (8/71); this rate during the same year as infection was 3.3 % (1/30), and the rate 4–17 years after HIV infection was 17.1 % (7/41). Approximately 91.2 % (145/159) of married women of childbearing age did not have children after being informed of HIV infection. Only 8.8 % (14/159) of these women had children after being informed of HIV infection. The mother-to-infant transmission rate was 38.5 % (5/13). The HAART coverage rate has increased from 10.1 % (16/159) in 2003 to 83.6 % (127/152) in 2013. Since 1999, the HIV mortality rate has trended up; by 2013, the cumulative mortality rate reached 54.9 % (179/326). After HAART was initiated in China, the death rate decreased to some extent. Second generation transmission (via couple or mother-to-infant transmission) among blood donor-related HIV cases accounted for approximately 4.0 % (13/326). All first- or second-generation cases were infected with HIV-1 subtype B. Conclusions In this accident of HIV-infection among plasma donors in Hebei Province, a total of 339 direct and second-generation cases have been identified over 18 years of monitoring. Favorable clinical results have been achieved using intervention measurements and antiviral therapy.

With respect to the storage allocation of cloud storage, this paper presents a new method to solve the problem based on improved genetic algorithm. The model is the storage efficiency of cloud storage systems as the goal, to achieve load balancing, high efficient and storage security. Firstly, preprocess the storage tasks and nodes during the storage task allocation, and manage virtual tasks and nodes after preprocessing in a whole. And then apply the natural number encoding to encode the storage task, combine with storage efficiency matrix for the individual choice, and improve the crossover operator and mutation operator. Simulation results show that this strategy can achieve the cloud storage allocation effectively.

Nitrogen-use efficiency of indica varieties of rice is superior to that of japonica varieties. We apply 16S ribosomal RNA gene profiling to characterize root microbiota of 68 indica and 27 japonica varieties grown in the field. We find that indica and japonica recruit distinct root microbiota. Notably, indica-enriched bacterial taxa are more diverse, and contain more genera with nitrogen metabolism functions, than japonica-enriched taxa. Using genetic approaches, we provide evidence that NRT1.1B, a rice nitrate transporter and sensor, is associated with the recruitment of a large proportion of indica-enriched bacteria. Metagenomic sequencing reveals that the ammonification process is less abundant in the root microbiome of the nrt1.1b mutant. We isolated 1,079 pure bacterial isolates from indica and japonica roots and derived synthetic communities (SynComs). Inoculation of IR24, an indica variety, with an indica-enriched SynCom improved rice growth in organic nitrogen conditions compared with a japonica-enriched SynCom. The links between plant genotype and root microbiota membership established in this study will inform breeding strategies to improve nitrogen use in crops.Rice coordinates recruitment of the root microbiota to optimize nitrogen acquisition from soil.

Media supplementation with exogenous chemicals is known to stimulate the accumulation of important lipids produced by microalgae and thraustochytrids. However, the roles of exogenous chemicals in promoting and preserving the terpenoids pool of thraustochytrids have been rarely investigated. Here, we realized the effects of two media supplements—mannitol and biotin—on the biomass and squalene production by a thraustochytrid strain (Thraustochytrium sp. ATCC 26185) and elucidated their mechanism of action. A significant change in the biomass was not evident with the exogenous addition of these supplements. However, with mannitol (1 g/L) supplementation, the ATCC 26185 culture achieved the best concentration (642 ± 13.6 mg/L) and yield (72.9 ± 9.6 mg/g) of squalene, which were 1.5-fold that of the control culture (non-supplemented). Similarly, with biotin supplementation (0.15 mg/L), the culture showed 459 ± 2.9 g/L and 55.7 ± 3.2 mg/g of squalene concentration and yield, respectively. The glucose uptake rate at 24 h of fermentation increased markedly with mannitol (0.31 g/Lh−1) or biotin (0.26 g/Lh−1) supplemented culture compared with non-supplemented culture (0.09 g/Lh−1). In addition, the reactive oxygen species (ROS) level of culture supplemented with mannitol remained alleviated during the entire period of fermentation while it alleviated after 24 h with biotin supplementation. The ∆ROS with mannitol was better compared with biotin supplementation. The total antioxidant capacity (T-AOC) of the supplemented culture was more than 50% during the late stage (72–96 h) of fermentation. Our study provides the potential of mannitol and biotin to enhance squalene yield and the first lines of experimental evidence for their protective role against oxidative stress during the culture of thraustochytrids.