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The study aimed to determine the relationship between digit ratios among a mother–child population in Ghana. This was a cross-sectional study from December 2020 to April 2021 involving 272 mothers, their daughters (n = 132) and their sons (n = 140). The right (2D:4DR) and the left (2D:4DL) digit ratios were measured using computer-assisted analysis. The data were analysed in SPSS (v23) and GraphPad Prism (v8) at an alpha value of 0.05. The mean ± SD age of the mothers was 23.9 ± 3.67 years while the median (IQR) age of daughters was 116(54–240) days and sons, 134(54–240) days. The mean ± SD 2D:4DR were 0.94 ± 0.04, 0.91 ± 0.04 and 0.90 ± 0.04 respectively for mothers, daughters and sons. The mean ± SD 2D:4DL was 0.93 ± 0.04, for mothers, 0.92 ± 0.05 for daughters and 0.92 ± 0.05 for sons. The daughters and sons showed leftward asymmetry while the mothers showed rightward asymmetry in digit ratios. The 2D:4DR of sons was significantly lower than daughters (P = 0.031). There were negative correlations between the 2D:4DL and age of daughters (r = −0.182, P = 0.043) and sons (r = −0.221, P = 0.012). The 2D:4DR of mothers was positively correlated with that of daughters (r = 0.332, P = 0.000) and that of sons (r = 0.233, P = 0.008). There are significant relationships between digit ratios in a mother–child population.

Pathophysiologically, type 2 diabetes can result from insulin resistance or insulin insufficiency alone. It is unclear whether relative insulin shortage or pronounced insulin resistance is linked to poor cardiometabolic problems like obesity. Therefore, the objective of this study was to evaluate the relationship between insulin resistance (IR), hypertension, and dyslipidaemia, in men with type 2 diabetes mellitus. One hundred and twenty-one (121) type 2 diabetic men participated in this cross-sectional study, which was conducted between September 2018 and September 2019. Sociodemographic information was collected using a self-designed questionnaire. Anthropometric data were also taken and blood samples collected for estimation of insulin, glucose, and lipid concentrations. HOMA-IR was calculated from the fasting insulin and glucose values, and a HOMA−IR≥2 was considered to indicate insulin resistance. Of the 121 participants, 39.7% were classified as insulin-resistant. Levels of total cholesterol (4.82±1.2 mmol/L; p=0.007 vs. 4.25±1.1 mmol/L), LDL cholesterol (3.17±0.9 mmol/L; p=0.001 vs. 2.52±0.8 mmol/L), and TC/HDL-C ratio (3.93±0.9; p=0.042 vs. 3.58±0.9) and the prevalence of abnormal LDL-C (14.6%; p=0.015 vs. 2.7%) and elevated BP (83.3%; p=0.048 vs. 67.1%) were higher in the insulin-resistant group. LDL cholesterol (AUC=0.670; p=0.001) better classified subjects as being insulin-resistant compared to other lipid markers. The odds of insulin resistance in dyslipidaemia were not statistically significant after adjusting for obesity. The link between insulin resistance and dyslipidaemia and hypertension in male diabetics may thus be mediated by obesity.

There are sex-dependent differences in hematological and biochemical variables in adulthood attributed to the predominant effects of testosterone in males and estrogen in females. The Twin Testosterone Transfer (TTT) hypothesis proposes that opposite-sex females may develop male-typical traits due to exposure to relatively higher levels of prenatal testosterone than same-sex females. Additionally, prenatal testosterone exposure has been suggested as a correlate of current circulating testosterone levels. Consequently, opposite-sex females might exhibit male-typical patterns in their hematological and biochemical variables. Despite this hypothesis, routine laboratory investigations assign the same reference range to all females. Our cross-sectional study, conducted in Tamale from January to September 2022, included 40 twins, comprising 10 opposite-sex (OS) males (25%), 10 OS females (25%), and 20 same-sex (SS) females (50%), all aged between 18 and 27 years. Fasting venous blood samples were collected and analyzed using automated hematology and biochemistry laboratory analyzers. Results indicated that levels of hemoglobin, serum creatinine, gamma-glutamyl transferase, total protein, globulins, and total testosterone were significantly higher in OS males than OS females. Conversely, total cholesterol and low-density lipoprotein cholesterol were significantly higher in OS females than OS males. Unexpectedly, levels of low-density lipoprotein cholesterol and total testosterone were significantly higher in SS females than OS females. Contrary to expectations, opposite-sex females did not exhibit male-typical patterns in their hematological and biochemical variables. This suggests that the TTT effect may not occur or may not be strong enough to markedly affect hematological and biochemical variables in OS females.

Background and Aims Preeclampsia poses a heightened risk for women, particularly in the development of hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome, leading to adverse outcomes for both mothers and newborns. The incidence of HELLP syndrome tends to be notably higher among women with preeclampsia compared with those with normotensive pregnancies. However, there is a dearth of research on the frequency of HELLP syndrome within the context of preeclampsia specifically in Ghana. Furthermore, the potential predictive value of serum erythrocyte adenylate kinase (EAK), a marker of hemolysis, in anticipating the onset of preeclampsia remains largely unexplored. Methods Conducted between May 2020 and April 2022, this research employed a case‐control methodology at the War Memorial and Upper East Regional Hospitals. A total of 291 pregnant women participated, comprising 111 diagnosed with preeclampsia and 180 control subjects, aged between 18 and 43 years. Venous blood samples were collected and subjected to analysis for platelet count, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and EAK, utilizing automated analyzers, alongside the ELISA technique. Diagnosis of HELLP syndrome was established using the Mississippi triple‐class definition. Results The median serum ALT level (with interquartile range) was significantly elevated in the preeclampsia group compared with controls [20.0 (13.7–27.0) vs. 13.0 (9.4–18.6); p < 0.001]. Moreover, the frequency of Mississippi class 3 HELLP syndrome was notably higher among preeclampsia cases (2/111; 1.8%) compared with controls (1/180; 0.6%). Serum ALT emerged as the superior predictor of preeclampsia, outperforming LDH (with an area under the curve of 0.73 compared with 0.58). The sensitivity and specificity of ALT were measured at 47.8% and 87.2%, respectively. Conclusion Although the occurrence of HELLP syndrome in preeclampsia cases appears relatively low, it may escalate as the prevalence of preeclampsia is anticipated to rise in low and middle‐income nations.

Background/Aim Immune tolerance in the fetal–maternal junction is maintained by a balance in the Th1/Th2 system. Th1‐type immunity is associated with pro‐inflammatory cytokines and immune checkpoint molecules (ICMs) such as B7‐H1, while Th2‐type immunity is characterized by anti‐inflammatory cytokines and ICMs such as B7‐1. Any imbalance in the Th1/Th2 immune system may lead to adverse pregnancy outcomes such as pre‐eclampsia (PE). Hitherto, the potential of serum B7‐1 and B7‐H1 proteins as early markers of PE has not been explored in the Ghanaian population. Materials and Methods This was a case–control study from May 2020 to April 2022 at the War Memorial and the Upper East Regional Hospitals. The study involved 291 women, including 180 (61.9%) with normotensive pregnancy and 111 (38.1%) with PE. Venous blood samples were collected and assayed for blood cell count, serum interleukins (ILs)‐4, ‐6, ‐12, ‐18, and TNF‐α as well as serum B7‐1 and B7‐H1 proteins. Results The monocyte count (p = .007), the serum levels of IL‐18 (p = .035), TNF‐α (p = .001), and B7‐H1 (p = .006) were significantly higher in PE than in normotensive pregnancy. In addition, the monocyte count (p = .002), the serum levels of IL‐12 (p = .029), TNF‐α (p = .016), and B7‐1 (p = .009) levels were significantly higher in the third trimester than the second trimester PE. In predicting PE, the area under the curve of cytokines and ICMs ranged from 0.51 for IL‐6 to 0.62 for TNF‐α. Conclusion PE may be characterized by a dominant Th1‐type immunity with higher levels of pro‐inflammatory cytokines and B7‐H1 proteins, but these variables may not be suitable for predicting PE. There are changes in serum cytokines and immune checkpoint molecules B7‐1 and B7‐H1 in pre‐eclampsia (PE). However, these changes are not predictive of PE.

Background and Aim The are sex differences in cardiometabolic risk factors in type 2 diabetes mellitus (T2DM) as well as the age at disease onset. However, the impact of these risk factors on the age at onset of T2DM is less known in the Ghanaian population. An understanding of the differential impact of cardiometabolic risk factors on the age at onset on T2DM may lead to sex‐specific interventions in preventive and management strategies for T2DM. Methods The study was cross‐sectional from January to June 2019 at the Bolgatanga regional hospital. The study involved 163 T2DM patients (Female = 103, Male = 60), aged from 25 to 70 years. The body mass index (BMI) and the waist‐to‐hip ratio (WHR) were measured following standardized anthropometric techniques. Fasting venous blood samples were collected and analyzed for cardiometabolic risk factors including total cholesterol (TCHOL) and low‐density lipoprotein (LDL) cholesterol. Results While TCHOL was higher in males than females (mean [SD]: F = .78 [1.37], M = 4.27 [1.39]) and LDL higher in females than males (mean [SD]: [F = 4.33 [1.22], M = 3.87 [1.26]), these did not, however, attain conventional statistical significance for TCHOL (t = 1.985, p = 0.05) and LDL (t = 2.001, p = 0.05). There were however, significant interactions between sex and the age at disease onset on TCHOL (t = −2.816, p = 0.006) and LDL (t = −2.874, p = 0.005), which were independent of the BMI, WHR and disease duration. The relationship between the age at disease onset and that of TCHOL and LDL were positive in females but negative in males. Conclusion Fasting plasma TCHOL and LDL increases with increasing age at onset of T2DM in females but decreases in males. Strategies for the prevention and management of T2DM should be sex‐specific. Females with T2DM should be given more attention regarding their fasting plasma cholesterol (total) and LDL cholesterol as they are more likely than men to have increased levels of these lipids with increasing age at disease onset.

Highly active antiretroviral therapy (HAART) is known to cause lipid abnormalities such as dyslipidaemia in HIV-infected individuals. Yet, dyslipidaemia may not independently occur as it may be worsened by single nucleotide polymorphisms (SNPs) in lecithin cholesterol acyltransferase (LCAT) and lipoprotein lipase ( LPL ). This case–control study was conducted in three-selected hospitals in the Northern part of Ghana. The study constituted a total of 118 HIV-infected participants aged 19–71 years, who had been on HAART for 6–24 months. Dyslipidaemia was defined based on the NCEP-ATP III criteria. HIV-infected individuals on HAART with dyslipidaemia were classified as cases while those without dyslipidaemia were grouped as controls. Lipid profile was measured using an automatic clinical chemistry analyzer and genomic DNA was extracted for PCR (GeneAmp PCR System 2700). Overall, the prevalence of dyslipidaemia was 39.0% (46/118). High levels of low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and reduced levels of high-density lipoprotein cholesterol (HDL-C) were observed in all cases. A total of 256 selected PCR amplicons comprising 137 LPL (exons 3, 5 and 6) and 119 LCAT (exons 1, 4, and 6) were sequenced in 46 samples (Inqaba Biotech). Six (6) clinically significant SNPs were identified in exons 1 and 4 for LCAT whereas 25 non-clinically significant SNPs were identified for LPL in exons 5 and 6. At position 97 for LCAT exon 1, there was a deletion of the nucleotide, ‘A’ in 32.5% (13/40) of the sampled population while 67.5% (27/40) of the sample population retained the nucleotide, ‘A’ which was significantly associated with dyslipidaemic outcomes in the study population (p = 0.0004). A total of 25 SNPs were identified in exons 5 and 6 of LPL ; 22 were substitutions, and 3 were insertions. However, none of the 25 SNPs identified in LPL exon 5 and 6 were statistically significant. SNPs in LCAT may independently contribute to dyslipidaemia among Ghanaian HIV-infected individuals on HAART, thus, allowing genetic and/or functional differential diagnosis of dyslipidaemia and creating an opportunity for potentially preventive options.

Background and Aims Type 2 diabetes mellitus (T2DM) is often linked to oxidative stress resulting from lipid peroxidation and complications such as diabetic retinopathy, neuropathy, nephropathy, and ketoacidosis (DKA), which may manifest differently based on sex. However, the specific relationship between diabetic complications and markers of oxidative stress in relation to sex remains uncertain. This study seeks to elucidate sex‐based variances in the association between oxidative stress markers and diabetic complications among individuals with T2DM in the Ghanaian population. Methods This study was conducted between July and December 2022 at the Tamale Teaching Hospital. The study involved 55 T2DM patients, comprising: no complications = 13; DKA = 13; neuropathy = 6; nephropathy = 14; and retinopathy = 9. The patients were aged between 20 and 55 years. Single fasting (8–10 h) venous blood samples were collected and analyzed for total cholesterol (TCHOL), high‐density lipoprotein cholesterol (HDL‐C), low‐density lipoprotein cholesterol (LDL‐C), malondialdehyde (MDA), and glutathione peroxidase (GSH‐Px) using an automated biochemistry analyzer and ELISA technique. Results While no significant sex differences were found in complication prevalence, notable disparities emerged in glycemic and oxidative stress markers. Males with DKA exhibited higher MDA levels compared to females, while females with retinopathy had lower MDA levels than males. Additionally, males with DKA showed higher GSH_Px levels than females, while no significant differences were observed between sexes without complications, and males with DKA and retinopathy tended to have lower HDL‐C levels compared to females. Conclusion While no significant differences in complication prevalence were observed between sexes, our findings revealed notable sex disparities in glycemic and oxidative stress markers. These results indicate the importance of considering sex‐specific factors in understanding the pathogenesis and management of diabetic complications.

The second‐to‐fourth digit ratio (2D:4D) is the putative marker of prenatal hormone exposure. The 2D:4D ratio or the right–left difference (Dr‐l) are said to be negative and positive correlates, respectively, of circulating testosterone and estrogen in both adult males and females. However, previous studies on the subject have reported mixed results. This study aimed to determine the sex‐moderated relationship between the 2D:4D ratio and adult circulating testosterone, estradiol, testosterone‐to‐estradiol ratio and the free androgen index. This was a cross‐sectional study from January to June 2021 at the University for Development Studies, Ghana. The study involved 62 participants (Female = 28; Male = 34), aged between 20 and 26 years. The right (2D:4DR), the left (2D:4DL), and their difference (Dr‐l) were measured by computer‐assisted analysis. Fasting venous samples were assayed for total testosterone (T), estradiol (E2), and sex hormone‐binding globulin (SHBG) using ELISA. The free androgen index (FAI) was then calculated (T/SHBG) and the data were analyzed using moderated and/or weighted regression. Males had significantly higher T and FAI than females while females had significantly higher E2 than males, which were independent of age and body mass index (p < 0.001). There was a significant SEX*Dr‐l interaction on FAI (p = 0.007). The Dr‐l correlated negatively with FAI in males but positively in females and accounted for about 94.0% of the variability of FAI in males (adjR2 = 0.940) and only 0.2% in females (adjR2 = 0.002). The 2D:4D ratio, a putative marker of prenatal hormone exposure, may have an impact on sex differences in adult free androgen index. The relationship between the index of prenatal hormone exposure (2D:4D) and the free androgen index is moderated by sex.

The 2D:4D ratio is the putative marker of prenatal hormone exposure and has been suggested as a correlate of adult circulating testosterone and estrogen. The study aimed to determine whether sexual dimorphism in the estimated glomerular filtration rate (eGFR) can be partly explained by the 2D:4D ratio or adult circulating testosterone or estrogen. The study was cross‐sectional from June to December 2021 at the University for Development Studies. The study involved 206 healthy adults (Female = 93, Male = 113) between 18 and 30 years. The 2D:4D ratio was measured using computer‐assisted analysis. Venous blood samples were collected and analyzed for testosterone, estradiol and creatinine using the ELISA technique and routine biochemical analysis. The adjusted eGFR was calculated using the Chronic Kidney Disease Epidemiology Collaboration (CKD‐EPI) creatinine equation (2021). The eGFR and the testosterone‐to‐estradiol ratio (TT:E2) were significantly higher in males than in females (p < 0.001). There was a significant interaction between sex and the TT:E2 on the eGFR (p < 0.001). Although the relationship between the eGFR and the TT:E2 was negative in both males and females, a unit change in the TT:E2 had a greater impact on the eGFR in females (B = −1.38) than in males (B = −0.01). Sexual dimorphism in the eGFR is influenced by both testosterone and estradiol. Although the sex difference in the eGFR may be influenced by the TT:E2, estrogen seems to account for more variability in the eGFR than testosterone. The testosterone‐to‐estradiol ratio may partly account for the sexual dimorphism in the estimated glomerular filtration rate among healthy adults.