Explore the vast range of titles available.

Background Urbanization, industrialization, and various other anthropogenic and natural activities increase the release of heavy metals (HMs) into the environment, posing a severe threat to nearby flora, fauna, and humans. HMs are toxic, non-biodegradable, silent, and subtle killers that alter the soil attributes and threaten plants, animals, and humans, impacting biodiversity and human health. Objective HMs such as Cd, Hg, and Pb disturb physiological processes in plants and animals, reducing growth, impaired reproduction, and negative impacts on human beings upon bioaccumulation in food chains. This results in the dire need for effective remediation strategies to clean up HMs in soil and water. Methods Various conventional and non-conventional approaches remove and detoxify HMs from the substrate. Metal immobilization, detoxification, and removal via microbial approaches, i.e., biosorption, bioaccumulation, bioaugmentation, bioleaching, and bio-volatilization, are effective in remediating Cd, Hg, and Pb. Results   Additionally, microbes utilize direct and indirect mechanisms to help the remediation of Cd, Hg, and Pb from contaminated sites. Genetically engineered microbes (GEMs) are also being explored for the remediation of these metals from contaminated soil, offering both opportunities and challenges regarding associated risks and management practices. This review emphasizes the possible heavy metal sources responsible for increasing the concentration of HMs in the environment and their effect on plants and animals. We will discuss various conventional remediation strategies that can help remediate Cd, Hg, and Pb. Conclusion By elucidating the role of microbial-dependent remediation approaches, including GEMs, this review also contributes to advancing sustainable approaches for mitigating Cd, Hg, and Pb-contaminated soil and safeguarding environment.

Anthropogenic activities have resulted in severe environmental degradation. Untreated wastewater from tanneries is hazardous to all kinds of life on earth. Effluent from tanning industries, containing large amount of Cr, is used to irrigate the crops in Pakistan. The current experiment was carried out to study the effects of tannery wastewater on spinach and the role of lysine-Zn in mitigating the severity of stress. The plants were grown in soil and the following treatments were used: irrigation with 0%, 33%, 66%, and 100% wastewater (ww) along with two doses (0 mM, 10 mM) of Zn-lysine. Foliar application of zinc-lysine enhanced the plant growth, biomass, Zn contents, photosynthesis, and enzyme activities in different tissues of plant. Zinc-lysine (10 mM) considerably decreased the Cr content in roots and shoots, along with ameliorating the oxidative stress by enhancing the activities of antioxidant enzymes in plants. Addition of Zn-lys (10 mM) improved the plant height by 19%, root length by 57%, leaf dry weight by 19%, and root dry weight by33% under 100% Cr treatment. Zn-lys significantly reduces the oxidative stress and concentration of Cr as compared with the Cr treatments alone. Application of Zn-lys (10 mM) reduced the Cr contents in roots by 27 and 22 under 33 and 66% Cr treatment, respectively. Taken together, Zn-lys chelates efficiently ameliorated the toxic effects of chromium. Zn-lysine has the extravagant potential of mitigating the heavy metal toxicity without harming the normal growth and development of the plants.

This study encompasses isolation and screening of heavy metal-resistant fungal and bacterial strains from tannery solid waste (TSW). Twelve fungal strains and 25 bacterial strains were isolated from TSW. The growth of fungal strains was observed against different heavy metals ranging from 10 to 1050 mg L−1 and the growth of bacteria was observed in metal concentrations ranging from 10 to 1200 mg L−1. Five multi-metal-resistant fungal isolates belonging to the genus Trichoderma and ten bacterial isolates belonging to the genus Bacillus showed good metal resistance and biosorption potential. They were identified through molecular techniques, fungi based on ITS region ribotyping, and bacteria based on 16S rRNA ribotyping. The fungal strains were characterized as T. hamatum (TSWF-06), T. harzianum (TSWF-11), T. lixii (TSWF-02), and T. pseudokoningii (TSWF-03, TSWF-10). The bacterial strains were characterized as Bacillus xiamenensis (TSW-02), B. velezensis (TSW-05), B. piscis (TSW-06), B. safensis (TSW-10), B. subtilis (TSW-14, TSW-15, TSW-17) B. licheniformis (TSW-19), B. cereus (TSW-20), and B. thuringiensis (TSW-22). The fungal strains, namely, T. pseudokoningii (TSWF-03) and T. harzianum, proved to be two multi-metal-resistant strains with good biosorption efficiency. Unlike fungi, bacterial strains showed metal-specific resistance. The strains Bacillus xiamenensis, B. subtilis (TSW-14), and B. subtilis (TSW-15) showed good biosorption efficiency against Cr, B. safensis against Cu, B. piscis, and B. subtilis (TSW-17) against Pb and B. licheniformis and B. thuringiensis against Zn. The autochthonous fungal and bacterial strains can therefore be employed to clean metal-contaminated environments.

This study was aimed at finding the metal sorption potential of six indigenous Trichoderma strains by using batch experiments for Cd (II), Cr (VI), Cu (II), and Pb (II). Trichoderma atrobrunneum showed maximum metal biosorption potential at 800 mg L −1 of initial concentration. Two adsorption isotherm models, (1) Langmuir (2) Freundlich models, were employed on the biosorption data obtained at various initial metal concentrations (10 mg L −1 –200 mg L −1 ) and pseudo-first (PSI) and pseudo-second (PSII) order equilibrium kinetic models were subjected to data of agitation time (3–7 days). A maximum correlation coefficient value (R 2 ) of ≤ 1 was observed for the Langmuir and PSII model. Results revealed that pH 6–7 was the best for metal sorption, while metal removal efficiency was increased by increasing temperature (298 K, 303 K, 308 K, 313 K). The results of thermodynamic study parameters (∆G°, ∆H°, ∆S°) indicated that heavy metal biosorption by Trichoderma strains was an endothermic, spontaneous, and feasible process. Moreover, surface characterization analysis through SEM, BET, FTIR, and XRD showed that T . atrobrunneum and Trichoderma sp. could adsorb more metal ions when grown in high metal concentrations. The results indicate that living biomass of T . atrobrunneum and Trichoderma sp. is an effective multi-metal biosorbent that can be used for efficacious bioremediation of bio-treatment of heavy metal polluted wastewater.

Kasur is one of the hubs of leather industry in the Punjab, Pakistan, where chrome tanning method of leather processing is extensively being used. Chromium (Cr) accumulation levels in the irrigation water, soil, and seasonal vegetables were studied in three villages located in the vicinity of wastewater treatment plant and solid waste dumping site operated by the Kasur Tanneries Waste Management Agency (KTWMA). The data was interpreted using analysis of variance (ANOVA), clustering analysis (CA), and principal component analysis (PCA). Interpolated surface maps for Cr were generated using the actual data obtained for the 30 sampling sites in each of the three villages for irrigation water, soil, and seasonal vegetables. The level of contamination in the three villages was directly proportional to their distance from KTWMA wastewater treatment plant and the direction of water runoff. The highest level of Cr contamination in soil (mg kg −1 ) was observed at Faqeeria Wala (37.67), intermediate at Dollay Wala (30.33), and the least in Maan (25.16). A gradational variation in Cr accumulation was observed in the three villages from contaminated wastewater having the least contamination level (2.02–4.40 mg L −1 ), to soil (25.16–37.67 mg kg −1 ), and ultimately in the seasonal vegetable crops (156.67–248.33 mg kg −1 ) cultivated in the region, having the highest level of Cr contamination above the permissible limit. The model used not only predicted the current situation of Cr contamination in the three villages but also indicated the trend of magnification of Cr contamination from irrigation water to soil and to the base of the food chain. Among the multiple causes of Cr contamination of vegetables, soil irrigation with contaminated groundwater was observed to be the dominant one.

The current research encompasses utilization of peanut shells (PS) as feedstock for pyrolysis carried out at various temperatures (250, 400, and 550 °C) for deriving biochar, namely PS-BC250, PS-BC400, and PS-BC550. After analyzing the biochar types physicochemically, it was applied as a soil ameliorant for the growth of cucumber. The results showed that in prepared biochar type, bulk density, volatile contents, hydrogen, oxygen, and nitrogen content decreased, whereas pH, electrical conductivity, ash content, fixed carbon content, and surface area increased with the increasing temperature. Scanning electron microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) presented high porosity, re-orientation of vessels, and a greater number of aromatic compounds, respectively, for PS-BC prepared at 550 °C. On applying PS-BC250, PS-BC400, and PS-BC550 as amendments in potted soil at 2, 4, and 6% (w/w), it improved soil quality (viz pH, ECe, BD, and soil water holding capacity) and increased the yield of cucumber. Because of improved soil properties and crop yield, PS-BC550 at the rate of 4% (w/w) demonstrated a great potential for agricultural application while provisioning dual circular economic indicators in the form of diverting PS waste to an effective alternative of chemical fertilizer having intensive carbon footprints in cucumber production.

The present study aimed at converting meat industry waste, particularly waste bones and chicken feathers, into biochar to recycle valuable nutrients present in it, which ultimately become part of the municipal waste. The bone biochar (BB) and feathers biochar (FB) were prepared at 550 °C, and their potential was evaluated as an organic amendment for the growth of sunflower. The ash content (AC) and fixed carbon (FC) improved significantly in prepared biochars as compared to raw feedstock. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) analyses signaled the occurrence of various functional groups viz. amide group and hydroxyapatite, porosity, and multiple nutrients. Application of BB and FB in potted soil alone as well as in composites (1:1, 1:2, 2:1) at 1%, 3%, and 5% (w/w) and synthetic fertilizer significantly increased soil pH, electrical conductivity (ECe), organic matter (OM) and water holding capacity (WHC), while reducing the bulk density (BD). The growth of plants grown in soil treated with a 2:1 composite of feathers and bone biochar at 5% application rate showed significantly greater differences in plant height, total chlorophyll content, and plant dry weight than the control but was comparable to growth with chemical fertilizer, rendering it a potential alternative to chemical-based synthetic fertilizer.

Synergistic effect of biochar and microbes in soil enhances performance of plants. Hazardous tannery solid waste can be reduced by one-third in volume by conversion to biochar. A greenhouse trial was set up with soil having different doses of metal resistant microbe-impregnated biochar (MIBC) prepared from tannery solid waste. Consortia of autochthonous strains of Trichoderma and Bacillus were inoculated on BC and the behavior and fate of metals were evaluated for their bioavailability to sunflower. Sunflower was grown in pots for 80 days having six different amendments of tannery solid waste biochar (0–10% w/w) with and without Trichoderma and Bacillus consortia and its morphological and biochemical attributes as well as metal uptake were observed. The results illustrated that application of BC at 2% rate without inoculation increased the shoot length and dry biomass by 19.8% and 77.4%, respectively, while plant growth and performance were reduced at higher amendments of BC. However, application of MIBC with Trichoderma or/and Bacillus consortium significantly improved the plant attributes at all levels of amendment. The results indicated that MIBC having Trichoderma and Bacillus consortia at 10% rate increased shoot length and dry biomass by 65.3% and 516% compared to control without BC. Application of BC without inoculation reduced the uptake of Cu, Fe, and Ni and increased the mobilization of all other metals for uptake in sunflower. Mobilization and uptake of Cd, Cr, Cu, Ni, Pb, and Zn decreased with MIBC having Trichoderma and Bacillus consortia whereas that of Fe and Mg were noted. A considerable decrease in proline and total phenolic content was demonstrated by MIBC-grown sunflower. The data of metal fractionation in BC also supported the above findings. Therefore, MIBC can be used as a promising option for enhancing growth performance and ensuring the physiological safety of sunflower as an energy crop.

Hexavalent chromium is one of the most common heavy metal, prevalent industrial wastes. Biosorption techniques using active and dead microorganisms on biochar can be employed to remediate effluents heavy metals. This research encompasses the removal of Cr (VI) from metal solutions using biochar colonized by an indigenous Cr resistant strain of Trichoderma asperellum (OM990850). The fungus was used as live biosorbent (Lb) and dead biosorbent (Db) colonizing the rice husk biochar (BC), as well as without biochar. Batch biosorption experiments considering pH, contact time, and initial metal concentration were carried out to observe the efficiency and mechanism of the sorption. Removal efficiency of Cr (VI) by Lb colonized BC was the highest (98%), followed by Lb (94%). Additionally, the metal uptake capacity of Lb and Lb + BC (274.9, 300 mgg −1, respectively) was also high. The Cr (VI) sorption was high at lower pH (2.0–4.0 pH). Two adsorption isotherm models were employed to investigate the efficacy of the process. The Langmuir non-linear model was the best fit for Lb and Lb + BC, and BC treatments (R 2  = 0.978, 0.997, 0.986, respectively), while the Freundlich model was a better fit for Db, Db + BC treatments (R 2  = 0.982 and 0.982, 0.967, respectively). Pseudo-second-order kinetic model was found to be the best fit with the obtained experimental data. Trichoderma asperellum impregnated biochar proved to be an effective, sustainable, cost-effective and eco-friendly biosorbent for removing Cr (VI) from industrial effluents and future commercialization. Highlights A colonized biochar derived from rice husk was prepared using a Cr resistant strain of Trichoderma asperellum. The fungus colonized biochar was used for heavy metal removal both in live (BC+Lb) and dead (BC+Db) forms. Live biomass of fungus alone and on biochar showed the best biosorption according to Langmuir Model. Psuedo-second order kinetic model was observed to be the best fit for the experimental data.

Endophytic bacteria inhabit plant tissues such as roots, stems, leaves, fruits, and seeds and can multiply inside plant tissue without damaging them. This study involves the isolation, characterization, metabolic profiling, and effect of endophytic bacteria isolated from the roots of Scots pine ( Pinus sylvestris ), on the growth of sunflower. In the current study, fifteen isolates of endophytic bacteria were obtained from the roots of Scots pine, and their molecular characterization was performed using 16 s rRNA ribotyping. The molecular characterization revealed that the strains belonged to Bacillus spp., Pseudomonas spp., Micrococcus sp., Serratia sp., Enterobacter sp., Pantoea sp., Staphylococcus sp., and Microbacterium sp. Among the isolated strains, 9 strains showed positive results for ammonium production, 12 strains for calcium solubilization, 11 strains for magnesium solubilization, 5 strains for zinc solubilization, 12 strains for phosphate solubilization, 8 strains for potassium solubilization, 10 strains for indole acetic acid (IAA) production, 9 strains for siderophore, and 6 strains for hydrogen cyanide (HCN) production. The greenhouse experiment results demonstrated that all isolated endophytic bacteria improved the shoot length, dry weight, and chlorophyll content of sunflower, whereas a significant increase was observed by PS-3 ( Bacillus cereus ), PS-6 ( Serratia marcescens ), and PS-8 ( Pseudomonas putida ). Besides, the concentration of nitrogen, phosphorus, and potassium were also measured in sunflower shoots, and results asserted that bacterial inoculation increased the bioavailability of these essential nutrients to plants compared to uninoculated control. Thus, these endophytic bacteria could be used as an encouraging option to improve plant growth and performance.