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Aim： Abrus agglutinin （AGG） from the seeds of Indian medicinal plant Abrus precatorius belongs to the class II ribosome inactivating protein family. In this study we investigated the anticancer effects of AGG against human hepatocellular carcinoma in vitro and in vivo. Methods： Cell proliferation, DNA fragmentation, Annexin V binding, immunocytofluorescence, Western blotting, caspase activity assays and luciferase assays were performed to evaluate AGG in human liver cancer cells HepG2. Immunohistochemical staining and TUNEL expression were studied in tumor samples of HepG2-xenografted nude mice. Results： AGG induced apoptosis in HepG2 cells in a dose- and time-dependent manner. AGG-treated HepG2 cells demonstrated an increase in caspase 3/7, 8 and 9 activities and a sharp decrease in the Bcl-2/Bax ratio, indicating activation of a caspase cascade. Co-treatment of HepG2 cells with AGG and a caspase inhibitor or treatment of AGG in Bax knockout HepG2 cells decreased the caspase 3/7 activity in comparison to HepG2 cells exposed only to AGG. Moreover, AGG decreased the expression of Hsp90 and suppressed Akt phosphorylation and NF-κB expression in HepG2 cells. Finally, AGG treatment significantly reduced tumor growth in nude mice bearing HepG2 xenografts, increased TUNEL expression and decreased CD-31 and Ki-67 expression compared to levels observed in the untreated control mice bearing HepG2 cells. Conclusion： AGG inhibits the growth and progression of HepG2 cells by inducing caspase-mediated cell death. The agglutinin could be an alternative natural remedy for the treatment of human hepatocellular carcinomas.

Nanozymes, a type of nanomaterial with enzyme-like properties, are a promising alternative to natural enzymes. In particular, transition metal dichalcogenides (TMDCs, with the general formula MX2, where M represents a transition metal and X is a chalcogen element)-based nanozymes have demonstrated exceptional potential in the healthcare and diagnostic sectors. TMDCs have different enzymatic properties due to their unique nano-architecture, high surface area, and semiconducting properties with tunable band gaps. Furthermore, the compatibility of TMDCs with various chemical or physical modification strategies provide a simple and scalable way to engineer and control their enzymatic activity. Here, we discuss recent advances made with TMDC-based nanozymes for biosensing and therapeutic applications. We also discuss their synthesis strategies, various enzymatic properties, current challenges, and the outlook for future developments in this field.

Chronic myeloid leukemia is a stem cell disease with the presence of Philadelphia chromosome generated through reciprocal translocation of chromosome 9 and 22. The use of first- and second-generation tyrosine kinase inhibitors has been successful to an extent. However, resistance against such drugs is an emerging problem. Apart from several drug-resistant mechanisms, drug influx/efflux ratio appears to be one of the key determinants of therapeutic outcomes. In addition, intracellular accumulation of drug critically depends on cell membrane fluidity and lipid raft dynamics. Previously, we reported two novel cell-penetrating peptides (CPPs), namely, cationic IR15 and anionic SR11 present in tryptic digest of Abrus agglutinin. Here, the potential of IR15 and SR11 to influence intracellular concentration of imatinib has been evaluated. Fluorescent correlation spectroscopy and lifetime imaging were employed to map membrane fluidity and lipid raft distribution following peptide-drug co-administration. Results show that IR15 and SR11 are the two CPPs which can modulate membrane fluidity and lipid raft distribution in K562 cells. Both IR15 and SR11 significantly reduce the viability of CML cells in the presence of imatinib by increasing the intracellular accumulation of the drug.

Soil salinization is a serious problem for cultivation of rice, as among cereals rice is the most salt sensitive crop, and more than 40% of the total agricultural land amounting to approximately 80 million ha the world over is salt affected. Salinity affects a plant in a varieties of ways, including ion toxicity, osmotic stress and oxidative damage. Since miRNAs occupy the top place in biochemical events determining a trait, understanding their role in salt tolerance is highly desirable, which may allow introduction of the trait in the rice cultivars of choice through biotechnological interventions. High throughput sequencing of sRNAs in the root and shoot tissues of the seedlings of the control and NaCl treated Pokkali, a salt-tolerant rice variety, identified 75 conserved miRNAs and mapped 200 sRNAs to the rice genome as novel miRNAs. Expression of nine novel miRNAs and two conserved miRNAs were confirmed by Northern blotting. Several of both conserved and novel miRNAs that expressed differentially in root and/or shoot tissues targeted transcription factors like AP2/EREBP domain protein, ARF, NAC, MYB, NF-YA, HD-Zip III, TCP and SBP reported to be involved in salt tolerance or in abiotic stress tolerance in general. Most of the novel miRNAs expressed in the salt tolerant wild rice Oryza coarctata, suggesting conservation of miRNAs in taxonomically related species. One of the novel miRNAs, osa-miR12477, also targeted L-ascorbate oxidase (LAO), indicating build-up of oxidative stress in the plant upon salt treatment, which was confirmed by DAB staining. Thus, salt tolerance might involve miRNA-mediated regulation of 1) cellular abundance of the hormone signaling components like EREBP and ARF, 2) synthesis of abiotic stress related transcription factors, and 3) antioxidative component like LAO for mitigation of oxidative damage. The study clearly indicated importance of osa-miR12477 regulated expression of LAO in salt tolerance in the plant.

Background Poor filling of grains in the basal spikelets of large size panicles bearing numerous spikelets has been a major limitation in attempts to increase the rice production to feed the world’s increasing population. Considering that biotechnological intervention could play important role in overcoming this limitation, the role of cytokinin in grain filling was investigated based on the information on cell proliferating potential of the hormone and reports of its high accumulation in immature seeds. Results A comparative study considering two rice varieties differing in panicle compactness, lax-panicle Upahar and compact-panicle OR-1918, revealed significant difference in grain filling, cytokinin oxidase (CKX) activity and expression, and expression of cell cycle regulators and cytokinin signaling components between the basal and apical spikelets of OR-1918, but not of Upahar. Exogenous application of cytokinin (6-Benzylaminopurine, BAP) to OR-1918 improved grain filling significantly, and this was accompanied by a significant decrease in expression and activity of CKX , particularly in the basal spikelets where the activity of CKX was significantly higher than that in the apical spikelets. Cytokinin application also resulted in significant increase in expression of cell cycle regulators like cyclin dependent kinases and cyclins in the basal spikelets that might be facilitating cell division in the endosperm cells by promoting G1/S phase and G2/M phase transition leading to improvement in grain filling. Expression studies of type-A response regulator ( RR ) component of cytokinin signaling indicated possible role of OsRR3 , OsRR4 and OsRR6 as repressors of CKX expression, much needed for an increased accumulation of CK in cells. Furthermore, the observed effect of BAP might not be solely because of it, but also because of induced synthesis of trans-zeatin (tZ) and N 6 -(Δ 2 -isopentenyl)adenine (iP), as reflected from accumulation of tZR (tZ riboside) and iPR (iP riboside), and significantly enhanced expression of an isopentenyl transferase ( IPT ) isoform. Conclusion The results suggested that seed-specific overexpression of OsRR4 and OsRR6 , and more importantly of IPT9 could be an effective biotechnological intervention towards improving the CK level of the developing caryopses leading to enhanced grain filling in rice cultivars bearing large panicles with numerous spikelets, and thereby increasing their yield potential.

Low light intensity is a great limitation for grain yield and quality in rice. However, yield is not significantly reduced in low light tolerant rice varieties. The work therefore planned for comparative transcriptome profiling under low light stress to decipher the genes involved and molecular mechanism of low light tolerance in rice. At active tillering stage, 50% low light exposure for 1 day, 3 days and 5 days were given to Swarnaprabha (low light tolerant) and IR8 (low light sensitive) rice varieties. Illumina (HiSeq) platform was used for transcriptome sequencing. A total of 6,652 and 12,042 genes were differentially expressed due to low light intensity in Swarnaprabha and IR8, respectively as compared to control. CAB, LRP, SBPase, MT15, TF PCL1 and Photosystem I & II complex related gene expressions were mostly increased in Swarnaprabha upon longer duration of low light exposure which was not found in IR8 as compared to control. Their expressions were validated by qRT-PCR. Overall study suggested that the maintenance of grain yield in the tolerant variety under low light might be results of accelerated expression of the genes which enable the plant to keep the photosynthetic processes moving at the same pace even under low light.

High grain number is positively correlated with grain yield in rice, but it is compromised because of poor filling of basal spikelets in dense panicle bearing numerous spikelets. The phenomenon that turns the basal spikelets of compact panicle sterile in rice is largely unknown. In order to understand the factor(s) that possibly determines such spikelet sterility in compact panicle cultivars, QTLs and candidate genes were identified for spikelet fertility and associated traits like panicle compactness, and ethylene production that significantly influences the grain filling using recombinant inbred lines developed from a cross between indica rice cultivars, PDK Shriram (compact, high spikelet number) and Heera (lax, low spikelet number). Novel QTLs, qSFP1.1 , qSFP3.1 , and qSFP6.1 for spikelet fertility percentage; qIGS3.2 and qIGS4.1 for panicle compactness; and qETH1.2 , qETH3.1 , and qETH4.1 for ethylene production were consistently identified in both kharif seasons of 2017 and 2018. The comparative expression analysis of candidate genes like ERF3 , AP2 -like ethylene-responsive transcription factor, EREBP , GBSS1 , E3 ubiquitin-protein ligase GW2 , and LRR receptor-like serine/threonine-protein kinase ERL1 associated with identified QTLs revealed their role in poor grain filling of basal spikelets in a dense panicle. These candidate genes thus could be important for improving grain filling in compact-panicle rice cultivars through biotechnological interventions.

The rapid degradation, overexploitation, and encroachment of floodplain wetlands have led to considerable decline in fish diversity and production from these invaluable aquatic resources threatening livelihood of the dependent fishers. The climate change evident in the fast few decades has further aggravated the problem of eutrophication causing water stress and sedimentation leading to rampant macrophyte proliferation affecting ecological and economic functioning of these ecosystems. Macrophyte control and management needs serious attention for sustaining ecosystem services provided by these resources. In this direction, pen culture of grass carp Ctenopharyngodon idella as a biocontrol for macrophytes along with Indian major carps was implemented in a co-management mode in Beledanga, a typical floodplain wetland, a gradually shrinking, macrophyte dominated floodplain wetland in lower Ganga basin. Indian major carps Labeo catla (6.28±0.23g), Labeo rohita (5.1±0.12g), Cirrhinus mrigala (3.5±0.08g) were stocked in the ratio 4:3:3 at the rate of 20 Nos.m −2 in pens (0.1ha each) in triplicate. Grass carp (7.1±0.42g) was stocked in pen at the rate of 20Nos.m −2 in duplicate. The fishes were fed with pelleted feed twice a day at the rate of 2–3% of body weight. The seed was overwintered in pens for a period of 90 days from November 2019 to January 2020. Average weight recorded at the end of culture period was 25.13±1.70g, 18.11±0.63g, 14.53±0.87g, and 39.20±1.90g in L. catla , L. rohita , C. mrigala , and C. idella , respectively. The survival of fish ranged from 70 to 81%. Growth performance and feed utilization efficiency of grass carp were significantly higher ( p <0.05) compared to other carp species. The pen culture was found to be economically viable with a benefit cost ratio of 1.53. The fishes produced were released back into the open wetland as an additional input for culture-based fisheries. The intervention along with niche-based enhanced stocking led to 24% increase in the fish production from the wetland with grass carp contributing 20–22% of the total catch with 32% increase in revenue generated by the sale of fish within a short span of 1 year. The study successfully demonstrated technological suitability and economic feasibility of pen culture in this wetland and role of grass carp as a potential biocontrol species for macrophyte management. Grass carp stocked in open wetland grew to 0.8 to 1kg within 6 months and 2–2.3kg within a year and could utilize 40–45% of the submerged and emergent macrophytes. Integration and optimization of grass carp will not only aid in habitat management of macrophyte-choked wetlands but will also boost their small-scale fisheries by converting standing macrophyte biomass into protein-rich fish biomass. The enhanced production will also cater to nutritional and livelihood security of the dependent fishers.

Labeo bata fry were reared in high-density polyethylene (HDPE) pens at different stocking densities to determine growth, survival, feed utilization efficiency and economics in Borkona beel, Barpeta, Assam. Pens (500 m2 each) were stocked with fry at four stocking densities, 3 (SD3), 5 (SD5), 7 (SD7) and 9 (SD9) no. m−2 in triplicates. Feeding was performed twice a day at a rate of 3–5% body weight with floating pelleted feed containing 28% crude protein. Fish grew from 2.38 ± 0.30 g to 82.78 ± 3.18, 75.94 ± 0.89, 71.94 ± 0.89 and 61.81 ± 3.35 g at stocking densities of 3, 5, 7 and 9 no. m−2, respectively. Weight gain per cent ranged from 2491.85 ± 140.56 to 3371.33 ± 133.16 and specific growth rate from 1.41 ± 0.02 to 1.55 ± 0.02, both of which decreased with increasing stocking density. The net and gross yields increased with increasing stocking density and were highest at SD9. The benefit-cost ratio was maximum at SD7 (1.42), followed by SD9 (1.41). Post-pen culture, the monthly income of fishers increased by 6.10% (SD3) to 40.50% (SD9). Significant differences (p > 0.05) in water quality parameters were not observed between treatments (inside pens) and reference site (outside pen at 10 m distance). Weight gain exhibited a significant positive correlation with temperature (r = 0.92; p = 0.029) and alkalinity (r = 0.95; p = 0.014). The present study can provide impetus towards species diversification in pen enclosures, income enhancement of small-scale wetland fishers and sustainable ecosystem-based floodplain wetland fisheries management.

Labeo bata fry were reared in high-density polyethylene (HDPE) pens at different stocking densities to determine growth, survival, feed utilization efficiency and economics in Borkona beel, Barpeta, Assam. Pens (500 m[sup.2] each) were stocked with fry at four stocking densities, 3 (SD3), 5 (SD5), 7 (SD7) and 9 (SD9) no. m[sup.−2] in triplicates. Feeding was performed twice a day at a rate of 3–5% body weight with floating pelleted feed containing 28% crude protein. Fish grew from 2.38 ± 0.30 g to 82.78 ± 3.18, 75.94 ± 0.89, 71.94 ± 0.89 and 61.81 ± 3.35 g at stocking densities of 3, 5, 7 and 9 no. m[sup.−2] , respectively. Weight gain per cent ranged from 2491.85 ± 140.56 to 3371.33 ± 133.16 and specific growth rate from 1.41 ± 0.02 to 1.55 ± 0.02, both of which decreased with increasing stocking density. The net and gross yields increased with increasing stocking density and were highest at SD9. The benefit-cost ratio was maximum at SD7 (1.42), followed by SD9 (1.41). Post-pen culture, the monthly income of fishers increased by 6.10% (SD3) to 40.50% (SD9). Significant differences (p > 0.05) in water quality parameters were not observed between treatments (inside pens) and reference site (outside pen at 10 m distance). Weight gain exhibited a significant positive correlation with temperature (r = 0.92; p = 0.029) and alkalinity (r = 0.95; p = 0.014). The present study can provide impetus towards species diversification in pen enclosures, income enhancement of small-scale wetland fishers and sustainable ecosystem-based floodplain wetland fisheries management.