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"Berntsen, Garrett"
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The Indian Ocean region : a strategic net assessment
The Indian Ocean Region (IOR) is one of the most areas of the world in human terms. This study provides a comprehensive overview of the subregions and countries in the IOR, drawing heavily on a new country risk assessment model developed by Abdullah Toukan, a senior associate with the Burke Chair at CSIS.
Book
Protecting our protectors
With a historic number of female veterans in our generation, are they getting one-size-fits-all mental health care that has been tailored for men? Other goals that even our diametrically opposed political parties should be able to agree on include: *Appointing a national director of suicide prevention, to develop an overarching strategy for suicide prevention and mental health support and oversee its execution. *Filling the 1,000 mental health care jobs currently unfilled at the VA and improving incentives to recruit mental health professionals. *Increasing mental health care eligibility for combat veterans from five to 15 years.
Newspaper Article
Soldiers Protesting the War on Reddit Dishonor Their Oath of Service
A quick Google search turns up a number of resources that help soldiers with principled objections to war file petitions to exit the military. Debate and avenues for dissent were already wide open without the Reddit posts and will remain so. Since the president decided to take his case to Congress, there are Congressional office phones ringing from constituents sharing their opinions on the use of force in Syria.
Newspaper Article
Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.
Journal Article
Ultrafast structural changes within a photosynthetic reaction centre
Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. Here we use time-resolved serial femtosecond crystallography
1
using an X-ray free-electron laser
2
to observe light-induced structural changes in the photosynthetic reaction centre of
Blastochloris viridis
on a timescale of picoseconds. Structural perturbations first occur at the special pair of chlorophyll molecules of the photosynthetic reaction centre that are photo-oxidized by light. Electron transfer to the menaquinone acceptor on the opposite side of the membrane induces a movement of this cofactor together with lower amplitude protein rearrangements. These observations reveal how proteins use conformational dynamics to stabilize the charge-separation steps of electron-transfer reactions.
Time-resolved serial femtosecond crystallography is used to reveal the structural changes that stabilize the charge-separation steps of electron-transfer reactions in the photosynthetic reaction centre of
Blastochloris viridis
on a timescale of picoseconds.
Journal Article
Ultrafast structural changes within a photosynthetic reaction centre
Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. In this study we use time-resolved serial femtosecond crystallography using an X-ray free-electron laser to observe light-induced structural changes in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds. Structural perturbations first occur at the special pair of chlorophyll molecules of the photosynthetic reaction centre that are photo-oxidized by light. Electron transfer to the menaquinone acceptor on the opposite side of the membrane induces a movement of this cofactor together with lower amplitude protein rearrangements. These observations reveal how proteins use conformational dynamics to stabilize the charge-separation steps of electron-transfer reactions.
Journal Article
Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.
Design Type(s)
virus particle imaging objective
Measurement Type(s)
physicochemical characterization • X-ray diffraction data
Technology Type(s)
dynamic light scattering • X-ray free electron laser • electrospray ionisation gas-phase electrophoretic mobility molecular analysis • particle-tracking velocimetry
Factor Type(s)
Sample Characteristic(s)
Rice dwarf virus (isolate O)
Machine-accessible metadata file describing the reported data
(ISA-Tab format)
Journal Article
Data Descriptor
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a wellcharacterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 mu m diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 angstrom ngstrom were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.
Journal Article