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The vertical distribution of volatile organic compounds (VOCs) within the planetary boundary layer (PBL) is critical for understanding ozone (O3) formation, yet knowledge remains limited in complex urban environments. In this study, vertical measurements of 117 VOC species were conducted using an unmanned aerial vehicle (UAV) equipped with a VOC multi-channel sampling system, up to a height of 500 m in Shenzhen, China. Results showed that total VOC (TVOC) concentrations decreased with altitude in the morning, reflecting the influence of surface-level local emissions, but increased with height at midday, likely driven by regional transport and potentially stronger photochemical processes. Source apportionment revealed substantial industrial emissions across all altitudes, vehicular emissions concentrated near the surface, and biomass burning primarily impacting higher layers. Clear evidence of enhanced secondary formation of oxygenated VOCs (OVOCs) was observed along the vertical gradient, particularly at midday, indicating intensified photochemical processes at higher altitudes. These findings underscore the importance of considering vertical heterogeneity in VOC distributions when modeling O3 formation or developing measures to reduce emissions at different altitudes, and also demonstrate the potential of UAV platforms to provide high-resolution atmospheric chemical data in complex urban environments.

Generative artificial intelligence (GenAI) has rapidly emerged as a promising tool in health care. Despite its growing adoption, how physicians make use of it in medical practice has not been qualitatively studied. Existing literature has largely focused on theoretical applications or experimental validations, with limited insight into real-world physician engagement with GenAI technologies. The aim of this study was to leverage a fine-grained dataset at the query level to quantitatively examine how physicians incorporate GenAI into their clinical and research workflows. The primary objective was to analyze usage patterns over time and across physician demographics. A secondary goal was to assess potential risks to patient privacy arising from physicians' interactions with GenAI platforms. This study collected 106,942 query-and-answer pairs by 989 physicians between August 29, 2023, and April 16, 2024. We performed topic classification to identify the most prevalent use cases, examining how these use cases evolved over time and across demographics. We also developed sensitivity classifiers to detect personally identifiable information in physicians' queries to explore the potential privacy breach risks around physicians' use of GenAI. Approximately 40% (396/989) of the enrolled physicians were female, 45.9% (454/989) were younger than 25 years, and 54.1% (535/989) were between 25 and 56 years of age. The majority of them worked in clinical departments (680/989, 68.8%) or medical technology departments (127/989, 12.8%). Our classification-based quantitative analyses suggest the following. First, physicians use GenAI predominantly for medical research (64,379/106,942, 60.2%) rather than clinical practice (13,100/106,942, 12.25%). Second, physicians focus more on health care-related questions (rising from 64,165/106,942, 60% to 83,415/106,942, 78%) within the first 15% (16,041/106,942) of their query sequence. Third, the use of GenAI differed across physician demographics and features. Specifically, female physicians asked a larger proportion of clinical questions (female: 0.154 vs male: 0.108; P<.001) and administration questions (female: 0.027 vs male: 0.018; P<.001) than male physicians; younger physicians posed more clinical questions (age ≤25: 0.146 vs age ∈ (25, 40]: 0.115 vs age >40: 0.103; P<.001) but fewer research questions (age ≤25: 0.580 vs age ∈ (25, 40]: 0.607 vs age >40: 0.664; P<.001) than senior physicians; and physicians accessing GenAI via computers asked more research questions (computer: 0.637 vs mobile: 0.296; P<.001), whereas physicians using mobile devices asked more clinical questions (computer: 0.107 vs mobile: 0.264; P<.001). Fourth, only 2.68% (2866/106,942) of physician queries contained sensitive information, the majority of which were primarily derived from writing and editing. Physicians are actively integrating GenAI into their professional routines, primarily leveraging it for research but also increasingly for clinical support. Usage patterns vary significantly across demographic lines, including gender, age, and device preference. Despite the presence of sensitive information in some queries, the risk of privacy breaches appears to be low.

Rapidly accumulating evidence has now suggested that the long non-coding RNAs (LncRNAs), a large and diverse class of non-coding transcribed RNA molecules with diverse functional roles and mechanisms, play a major role in the pathogenesis of many human inflammatory diseases. Although some LncRNAs are overexpressed in plasma, T cell, and synovial tissues of patients with rheumatoid arthritis (RA), there is a dearth of knowledge in what role these transcripts play in fibroblast-like synoviocytes (FLSs) of these patients. Here, our studies showed that GAPLINC, a newly identified functional LncRNA in oncology, displayed a greater degree of expression in FLSs from RA than in patients with traumatic injury. GAPLINC suppression in RA-FLS cells revealed significant alterations in cell proliferation, invasion, migration, and proinflammatory cytokines production. Additionally, we performed a preliminary bioinformatics analysis of GAPLINC gene sequence in order to find its target molecules, using miRanda, PITA, RNAhybrid algorithms, Kyoto encyclopedia of genes and genomes, and gene ontology analysis. Since the results predicted that some of microRNAs and mRNA may interact with GAPLINC, we simulated a gene co-action network model based on a competitive endogenous RNA theory. Further verification of this model demonstrated that silencing of GAPLINC increased miR-382-5p and miR-575 expression. The results of this study suggest that GAPLINC may function as a novel microRNAs sponging agent affecting the biological characteristics of RA-FLSs. Additionally, GAPLINC may also promote RA-FLS tumor-like behaviors in a miR-382-5p-dependent and miR-575-dependent manner. Based upon these findings, LncRNA GAPLINC may provide a novel valuable therapeutic target for RA patients.

Background The ability of socially assistive robots (SARs) to treat dementia and Alzheimer’s disease has been verified. Currently, to increase the range of their application, there is an increasing amount of interest in using SARs to relieve pain and negative emotions among children in routine medical settings. However, there is little consensus regarding the use of these robots. Objective This study aimed to evaluate the effect of SARs on pain and negative affectivity among children undergoing invasive needle-based procedures. Design This study was a systematic review and meta-analysis of randomized controlled trials that was conducted in accordance with the Cochrane Handbook guidelines. Methods The PubMed, CINAHL, Web of Science, Cochrane Library, Embase, CNKI, and WanFang databases were searched from inception to January 2024 to identify relevant randomized controlled trials (RCTs). We used the Cochrane Risk of Bias tool 2.0 (RoB2.0) to assess the risk of bias among the included studies, and we used RevMan 5.4 software to conduct the meta-analysis. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) framework was used to assess the quality of the evidence. Results Ten RCTs involving 815 pediatric subjects were selected for this review and reported outcomes related to pain and emotions during IV placement, port needle insertion, flu vaccination, blood sampling, and dental treatment. Children undergoing needle-related procedures with SARs reported less anxiety (SMD= -0.36; 95% CI= -0.64, -0.09) and fewer distressed avoidance behaviors (SMD= -0.67; 95% CI= -1.04, -0.30) than did those receiving typical care. There were nonsignificant differences between these groups in terms of in pain (SMD = -0.02; 95% CI = − 0.81, 0.78) and fear (SMD = 0.38; 95% CI= -0.06, 0.82). The results of exploratory subgroup analyses revealed no statistically significant differences based on the intervention type of robots or anesthetic use. Conclusions The use of SARs is a promising intervention method for alleviating anxiety and distress among children undergoing needle-related procedures. However, additional high-quality randomized controlled trials are needed to further validate these conclusions. Trial registration The protocol of this study has been registered in the database PROSPERO (registration ID: CRD42023413279).

Although Guangxi represents one of the distribution centres of begonias in China, the sect. Diploclinium (Wright) A. DC is not well documented herein. In this article, we illustrate a new species belonging to this section, Begonia fangchengensis Y.N.Huang, X.X. Feng & R.K.Li, which has been discovered in southern Guangxi. Begonia fangchengensis mostly resembles B. rotundilimba in elliptic leaf blade, palmate veins, dichasial cyme, three styles, axile placentation, and unequally 3-winged capsule. On the other hand, B. fangchengensis is characterized by creeping rhizome, pilose stipule, red and densely pilose petiole, pubescent leaf blade and pilose inflorescence bract, sparsely pilose ovary and the zygomorphic androecium, differing from the erect stem at anthesis, the green and densely villous petiole, the glabrous stipule, leaf blade, inflorescence bract, ovary and actinomorphic androecium in B. rotundilimba . Considering its small population size and narrow distribution, its conservation status is categorized as ‘Endangered (D)’ according to the IUCN Red List Categories and Criteria.

Tropical cyclones have devastating impacts on the environment, economies, and societies and may intensify in the coming decades due to climate change. Stable water isotopes serve as tracers of the hydrological cycle, as isotope fractionation processes leave distinct precipitation isotopic signatures. Here we present a record of daily precipitation isotope measurements from March 2014 to October 2015 for Metropolitan Manila, a first-of-a-kind dataset for the Philippines and Southeast Asia. We show that precipitation isotopic variation at our study site is closely related to tropical cyclones. The most negative shift in δ18O values (−13.84 ‰) leading to a clear isotopic signal was caused by Typhoon Rammasun, which directly hit Metropolitan Manila. The average δ18O value of precipitation associated with tropical cyclones is −10.24 ‰, whereas the mean isotopic value for rainfall associated with non-cyclone events is −5.29 ‰. Further, the closer the storm track is to the sampling site, the more negative the isotopic values are, indicating that in situ isotope measurements can provide a direct linkage between isotopes and typhoon activities in the Philippines.

Rheumatoid arthritis (RA) is a progressive, chronic, even disabling systemic autoimmune disease. Imbalance between pathogenic immune cells and immunosuppressive cells is associated with the pathogenesis and development of RA and other autoimmune diseases. As Foxp3 is also expressed on activated CD4 cells in the presence of inflammation, the identification of Treg cells in patients with RA remains a challenge. Comprehensive analyses were carried out by Flow cytometry. Expression of Helios, CD226, T cell immunoreceptor with Ig and ITIM domains clinical samples and healthy controls. We have systemically examined three potential markers, Helios, CD226 and TIGIT, that are possibly related to Treg identification, and found that Helios expression on CD4 Foxp3 cells was decreased and negatively correlated with the disease activity of RA patients, while CD226 and TIGIT both showed elevated expression levels in CD4 Foxp3 cells in RA patients and they were not associated with disease activity of RA patients. Taken together, our findings indicate that CD4 CD25 CD127 Foxp3 Helios may represent the real Treg cell population in patients with RA.

The variability of the UV and optical Fe ii emission is investigated for a QSO, SDSS J142052.43+525622.4 (z=0.676), with 50 spectroscopic observations covering 1047 d in the observed frame. This QSO is selected from the Sloan Digital Sky Survey-Reverberation Mapping (SDSS-RM) project. Using multiple components to model the pseudo-continuum and emission lines (Mg ii, Hβ, [O iii], Fe ii), some results are outlined as follows: (i) Based on the distribution of the [O iii] 5007 Å luminosity, the flux-calibration uncertainty is about 7.8%. For the UV continuum, this QSO appears bluer when it gets brighter, while we cannot find this trend for the optical continuum. It is possible that there is a contamination from the hosts in the optical continuum. (ii) There is a strong correlation between the UV and optical Fe ii equivalent width, which shows an intimate connection between them. However, the full width at half-maximum (FWHM) of the UV Fe ii is generally larger than FWHM of the optical Fe ii. The redshift of the UV Fe ii is generally larger than for the optical Fe ii. These results show that the region emitting the UV Fe ii is located inside and has a larger inflow than the optical Fe ii. (iii) Based on the collisionally ionized model, the hydrogen column density is ∼1023 cm−2 calculated from the intensity ratio of the optical to UV Fe ii. This Fe ii intensity ratio has a strong relation with the UV/optical continuum spectral index. (iv) The intrinsic Baldwin effect for the UV/optical Fe ii is significant and the EW of the UV Fe ii decreases more quickly than the optical Fe ii when this QSO becomes bright.

Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammatory in joints. Invasive pannus is a characteristic pathological feature of RA. RA fibroblast-like synoviocytes (FLSs) are showed tumor-like biological characters that facilitate pannus generation. Importantly, it has been documented that extracellular vesicle (EVs) derived microRNAs have a vital role of angiogenesis in various immune inflammatory diseases. However, whether RA FLSs derived EVs can facilitate angiogenesis and the underlying mechanism is undefined. Herein, we aim to investigate the key role of RA FLSs derived EVs on angiogenesis in endothelial cells (ECs). We indicate that RA FLSs derived EVs promote ECs angiogenesis by enhancing migration and tube formation of ECs in vitro . Also, we confirm that RA FLSs derived EVs can significantly facilitate ECs angiogenesis with a matrigel angiogenesis mice model. In terms of the mechanisms, both RNAs and proteins in EVs play roles in promoting ECs angiogenesis, but the RNA parts are more fundamental in this process. By combining microRNA sequencing and qPCR results, miR-1972 is identified to facilitate ECs angiogenesis. The blockage of miR-1972 significantly abrogated the angiogenesis stimulative ability of RA FLSs derived EVs in ECs, while the overexpression of miR-1972 reversed the effect in ECs. Specifically, the p53 level is decreased, and the phosphorylated mTOR is upregulated in miR-1972 overexpressed ECs, indicating that miR-1972 expedites angiogenesis through p53/mTOR pathway. Collectively, RA FLSs derived EVs can promote ECs angiogenesis via miR-1972 targeted p53/mTOR signaling, targeting on RA FLSs derived EVs or miR-1972 provides a promising strategy for the treatment of patients with RA.

The new subfamily Sinopyrophorinae within Elateridae is proposed to accommodate a bioluminescent species, Sinopyrophorusschimmeli Bi & Li, gen. et sp. nov. , recently discovered in Yunnan, China. This lineage is morphologically distinguished from other click-beetle subfamilies by the strongly protruding frontoclypeal region, which is longitudinally carinate medially, the pretarsal claws without basal setae, the hind wing venation with a well-defined wedge cell, the abdomen with seven (male) or six (female) ventrites, the large luminous organ on the abdominal sternite II, and the male genitalia with median lobe much shorter than parameres, and parameres arcuate, with the inner margin near its apical third dentate. Molecular phylogeny based on the combined 14 mitochondrial and two nuclear genes supports the placement of this taxon far from other luminescent click-beetle groups, which provides additional evidence for the multiple origin of bioluminescence in Elateridae. Illustrations of habitus and main diagnostic features of S.schimmeli Bi & Li, gen. et sp. nov. are provided, as well as the brief description of its luminescent behavior.