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Protein lysine acetylation, a dynamic and reversible posttranslational modification, plays a crucial role in several cellular processes, including cell cycle regulation, metabolism, enzymatic activities, and protein interactions. Brenneria nigrifluens is a pathogen of walnut trees with shallow bark canker and can cause serious disease in walnut trees. Until now, a little has been known about the roles of lysine acetylation in plant pathogenic bacteria. In the present study, the lysine acetylome of B. nigrifluens was determined by high‐resolution LC‐MS/MS analysis. In total, we identified 1,866 lysine acetylation sites distributed in 737 acetylated proteins. Bioinformatics results indicated that acetylated proteins participate in many different biological functions in B. nigrifluens. Four conserved motifs, namely, LKac, Kac*F, I*Kac, and L*Kac, were identified in this bacterium. Protein interaction network analysis indicated that all kinds of interactions are modulated by protein lysine acetylation. Overall, 12 acetylated proteins were related to the virulence of B. nigrifluens. In the present study, the lysine acetylome of Brenneria nigrifluens was determined by high‐resolution LC‐MS/MS analysis. In total, we identified 1,866 lysine acetylation sites distributed in 737 acetylated proteins. Bioinformatics results indicate that acetylated proteins participate in many different biological functions in B. nigrifluens.

Despite prior studies have shown the impacts of different types of social media use on adolescent's mental health can vary, little is known about why and when these differences occur. Thus, this study investigated the effects of different types of social media use-public active use, private active use, and passive use-on adolescent mental health through Self-Determination Theory's three basic psychological needs (autonomy, relatedness, and competence), and how these effects are shaped by the quality of offline relationships. A two-wave panel study with five-month interval was conducted among 343 randomly selected Chinese adolescents aged 11 to 17 (from grade 7 to grade 11). Findings showed that no direct effects of any type of social media use on mental health outcomes over time, while private active social media use significantly predicted the fulfillment of relatedness needs and positively contributed to subsequent life satisfaction. The \"poor get richer\" and \"rich get richer\" of social media use hypotheses were supported. Specifically, for adolescents with low-quality offline relationships, public active social media use promoted the satisfaction of competence needs and thus decreased depression, and promoted competence and autonomy satisfaction and thus increased life satisfaction. For adolescents with high-quality offline relationships, private active social media use facilitated relatedness satisfaction, subsequently enhancing life satisfaction. This study introduces an explanatory boundary condition for the diversity of psychological reactions stemming from different types of social media use, and underscores the significance of personalized guidance for adolescents' social media use.

The use of video-conference interviews (VIs) has increased substantially, especially with advances in technology and the emergence of COVID-19. Although prior research has suggested that VIs are associated with a lower perception of fairness by applicants and less favorable ratings by interviewers in contrast to face-to-face (FTF) interviews, the reasons for these differences remain inadequately understood. We postulate that applicants’ and interviewers’ perceptions of the other party’s social presence may mediate the relationships among the interview medium, applicants’ perceptions of fairness and interviewers’ ratings. We also explore the moderating roles of applicants’ nonverbal communication expectations and interviewers’ construal level in these mediated relationships. We conduct a one-factor experimental design involving 232 participants (116 applicants and 116 interviewers) and find that VIs lead to lower perceptions of fairness by applicants and lower ratings by interviewers through lower perceptions by applicants and interviewers of the other party’s social presence. Additionally, applicants’ expectations for nonverbal communication during interviews moderate the relationship between the interview media and applicants’ experience of social presence. Specifically, applicants with high expectations for nonverbal communication experience greater social presence and fairness in FTF interviews than in VIs. For applicants with low expectations of nonverbal communication, however, VIs and FTF interviews do not differ in terms of social presence or fairness perceptions, and the moderating effect of interviewers’ construal level is not significant. We discuss the implications of these results for increasing applicants’ perceptions of fairness and the efficacy of interview evaluations in job interviews.

This study utilized 210 Malus sieversii germplasm fruit samples collected from seven natural populations, which exhibit rich genetic diversity in their hereditary traits. The research findings systematically evaluated and identified the genetic diversity of 31 fruit phenotypic traits using five statistical analysis methods: frequency analysis, quantitative trait statistical analysis, correlation analysis, principal component analysis (PCA), and cluster analysis, thereby enabling the screening of distinctive germplasm resources. Frequency distribution analysis revealed that a high proportion of the samples had fruit weight ranging from 10–20 g, fruit longitudinal diameters from 25–31 mm, fruit transverse diameters from 30–39 mm, pedicel length from 10–20 mm, and soluble solids content (SSC) from 11.4%-13.75%. The fruit shape of M. sieversii is predominantly “oblate” or “oblique”, with the ground color mostly “green-yellow”, flesh color mostly “green-white”, and flavor predominantly “sour”. Statistical analysis of quantitative traits revealed that the coefficient of variation for eight quantitative traits ranged from 7.02%-38.24%. Among these, fruit weight and pedicel length exhibited relatively high coefficients of variation, indicating rich genetic diversity. Correlation analysis showed that fruit weight was significantly positively correlated with fruit longitudinal diameters, fruit transverse diameters and pedicel diameter, while pedicel length was significantly negatively correlated with pedicel diameter. Fruit ground color showed significant negative correlations with flesh color and juiciness. PCA extracted 12 principal components with a cumulative contribution rate of 68.43%. Among these, the first four principal components - fruit size, flavor quality, basin morphology, and pedicel characteristics - were identified as the main dimensions constituting the phenotypic diversity of M. sieversii fruits. The 210 germplasm resources were classified into four clusters: Cluster I comprised small-fruit, high-acidity resources; Cluster II, resources with long pedicel traits; Cluster III, oblate-shaped, short-pedicel resources; and Cluster IV, specific resources characterized by large fruit size and favorable flavor quality. The results provide a theoretical basis for the effective utilization of M. sieversii germplasm resources and offer references for the selection and breeding of specific resources.

A Gram-negative, facultatively anaerobic, motile bacterial strain, TPQG1-4T, was isolated from the leaf of Cyclobalanopsis patelliformis with spot disease. The isolate was investigated using the polyphasic taxonomic approach. 16S rRNA gene sequencing and analyzing revealed that the novel strain shares the highest sequence similarity with Stenotrophomonas lactitubi M15T (99.6%), Stenotrophomonas indicatrix WS40T (99.4%), Stenotrophomonas maltophilia IAM 12423T (99.2%) and Stenotrophomonas pavanii LMG 25348T (99.0%). In phylogenetic trees based on 16S rRNA gene sequences, the novel strain branched independently from other species of Stenotrophomonas. Average nucleotide identity values between the novel isolate and S. lactitubi M15T, S. indicatrix WS40T, S. maltophilia IAM 12423T, S. pavanii LMG 25348T, and Pseudomonas geniculata ATCC 19374T were 87.2%, 87.3%, 86.3%, 88.0%, and 81.3%, respectively, suggesting the isolate was a novel species of the genus Stenotrophomonas. The DNA G + C content of TPQG1-4T is 67.1 mol%. The major fatty acids were iso-C15:0 (25.4%) and anteiso-C15:0 (17.0%). The polar lipids of TPQG1-4T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, amino phospholipid and phospholipid. Based on phenotypic and genotypic characteristics, the strain represents a novel species in the genus Stenotrophomonas, for which the name Stenotrophomonas cyclobalanopsidis sp. nov. is proposed. The type strain is TPQG1-4T (= CFCC 15341T = LMG 31208T).

L3-3HA T , a Gram-negative-staining, facultatively anaerobic, motile bacterial strain, was isolated from the symptomatic bark of Salix matsudana canker in China. 16S rRNA gene analysis revealed that the novel strain shares the highest sequence similarity with Brenneria goodwinii FRB141 T (95.5%). In phylogenetic trees based on four housekeeping genes ( gyr B, rpo B, atp D, and inf B) and the 16S rRNA gene sequence, the novel strain formed a separate branch from the five genera of the family Pectobacteriaceae ( Lonsdalea , Brenneria , Dickeya , Pectobacterium , and Sodalis ), suggesting that the novel strain should belong to a novel species of a novel genus within the family Pectobacteriaceae . The result was also supported by phylogenomics, amino acid identity and average nucleotide identity. The major fatty acids were C 14:0 , C 16:0 , C 17:0 cyclo, and C 19:0 cyclo ɷ 8 c . Genome analysis showed that the novel strain has a large genome (5.89 Mb) with 5,052 coding genes, including 181 virulence genes by searching the pathogen–host interactions database (PHI-base), indicating that the novel strain is a potential pathogen of plants and animals. Based on phenotypic and genotypic characteristics, the L3-3HA T strain represents a novel species of a novel genus in the Pectobacteriaceae family, for which the name Affinibrenneria salicis gen nov. sp. nov. is proposed. The strain type is L3-3HA T (= CFCC 15588 T  = LMG 31209 T ).

Gnomoniopsis (Gnomoniaceae, Diaporthales) is a well-classified genus inhabiting leaves, branches and fruits of the hosts in three plant families, namely Fagaceae, Onagraceae and Rosaceae. In the present study, eighteen Gnomoniopsis isolates were obtained from diseased leaves of Fagaceae hosts collected from Fujian, Guangdong, Hainan, Henan, Jiangxi and Shaanxi provinces in China. Morphology from the cultures and phylogeny based on the 5.8S nuclear ribosomal DNA gene with the two flanking internally transcribed spacer (ITS) regions, the translation elongation factor 1-alpha (tef1) and the beta-tubulin (tub2) genes were employed to identify these isolates. As a result, seven species were revealed, viz. Gnomoniopsis castanopsidis, G. fagacearum, G. guangdongensis, G. hainanensis, G. rossmaniae and G. silvicola spp. nov, as well as a known species G. daii. In addition, G. daii was firstly reported on the host Quercus aliena.

The traditional self-report instruments (eg, scales) used to measure antisocial personality traits are characterized by social desirability bias and fail to capture multidimensional behaviors (eg, manipulation and deception). This study aimed to develop and validate an evidence-based design for a gamified assessment tool (Antisocial Personality Traits Evidence-Centered Design Gamified assessment tool; ASP-ECD-G) to measure 7 antisocial personality traits (manipulative, callous, deceptive, hostile, risk taking, impulsive, and irresponsible) as defined in the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5). This research featured a 3-phase evidence-centered design framework. Ontology development (study 1): semistructured interviews were conducted with 9 workplace professionals to translate the DSM-5 criteria into 24 observable workplace behaviors, which were integrated into a text-based game featuring 10 subscenarios, 34 interactive questions, and logic rooted in logical jumps to simulate real-world decision-making. Model construction (study 2): 6 machine learning models were trained by reference to a set of Personality Inventory for DSM-5 Short Form scores (n=286). The gated recurrent unit model, which uses 1-hot encoding to address nominal response data, was evaluated in terms of the root mean square error (RMSE), mean absolute error, criterion correlation (r), and test-retest reliability. Retest reliability was assessed using intraclass correlation coefficients based on 10 participants (1-month interval). Empirical validation (study 3): a 2×2 mixed design (n=148) was used to compare the gamified assessment with questionnaires under conditions involving incentives (ie, situations in which \"rational results\" led to increased payments). For model performance, the gated recurrent unit outperformed the alternatives, as indicated by the highest criterion correlation (r=0.850) and the lowest test RMSE (0.273); in particular, it excelled in moderate score ranges (1.5-3, RMSE≤0.377) and in resisting extreme value distortions (3.5-4, RMSE 0.854). Retest reliability was moderate to strong (intraclass correlation coefficients=0.776, P=.02). For validation findings, the gamified assessment was associated with higher levels of immersion (mean 7.628 vs 7.216; F147=14.259, P<.001) and interest (mean 7.095 vs 6.155; F147=47.940, P<.001), although it also elicited stronger negative emotions (mean 4.365 vs 2.473; F147=151.109, P<.001). Incentives reduced questionnaire scores (incentivized: 2.066 vs control: 2.201; F1=5.740, P=.02) but had no effect on gamified scores (P=.71), confirming resistance to manipulation. By integrating evidence-centered design with gamified workplace simulations, ASP-ECD-G can provide more objective and ecologically valid measurements of antisocial personality traits, thereby supporting both research and organizational practice. Open Science Framework (OSF) Registries tvg6x; https://osf.io/tvg6x.

The Ili region hosts China’s largest lavender cultivation base, yet soil bacterial diversity in its primary cultivation areas remains understudied. To address this, we compared soil bacterial communities across four major cultivation counties (Chabuchar, Agricultural Research Institute, Yining, and Huocheng). Essential oil profiles, soil properties, and bacterial community characteristics were analysed to elucidate microbial variations and environmental interactions. The results showed that: (1) The essential oil yield (1.14%) and linalool content (41.04%) in the Huocheng County cultivation area were significantly higher than those in other areas, and the essential oil quality was relatively the best; (2) the soil bacterial communities in different main cultivation areas shared certain commonalities. At the phylum level, Proteobacteria, Acidobacteriota, Gemmatimonadota, and Actinobacteriota were the dominant phyla, and their relative abundances varied by region and soil layer, and (3) the redundancy analysis results showed that soil bacterial communities were comprehensively affected by environmental factors such as pH, total nitrogen, total phosphorus, soil organic carbon, longitude, and altitude. The significant positive correlations between the abundance of Vicinamibacteraceae (Acidobacteriota) in Huocheng County soils and both soil total phosphorus and linalool content suggest a putative mechanism whereby this bacterial taxon enhances lavender terpenoid synthesis by facilitating phosphorus cycling. Overall, these results suggest that geographically driven climatic variations dynamically alter the soil bacterial community, thereby influencing lavender growth and the final essential oil quality.

Strain DKSPLA3T, a novel Gram-negative, catalase-positive, oxidase-positive, non-spore-forming, aerobic, non-nitrogen-fixing, non‐motile bacterium was isolated from Quercus variablis leaf, in Zunyi, Guizhou, China. Growth occurred at 4–37 °C (optimum 28 °C), pH 4.0–9.0 (optimum pH 7.0) and up to 4.0% (w/v) NaCl (optimum under 2.0%, w/v). Phylogeny based on 16S rRNA gene indicated that strain DKSPLA3T was a novel species in the genus Rhizobium, which was supported by average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values. The predominant fatty acids of strain DKSPLA3T were C16:0, C18:1ω7c and/or C18:1ω6c and C18:1ω7c 11-methyl. The major respiratory quinone was Q-10. Major polar lipids were diphosphatidyl glycerol (DPG), phosphatidyl glycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), phosphatidylcholine (PC), two unidentified phospholipids (PL) and nine unidentified lipids (L). The genomic G + C content was 64.47 mol%. Based on the phenotypic, phylogenetic and genotypic data, DKSPLA3T should be classified as a novel species in the genus Rhizobium, for which the name Rhizobium quercicola sp. nov. (KCTC 82843T = CFCC 16,707T) is proposed.