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"Birdsall, E"
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Privilege through the looking-glass
\"Privilege Through the Looking-Glass is a collection of original essays that explore privilege and status characteristics in daily life. This collection seeks to make visible that which is often invisible. It seeks to sensitize us to things we have been taught not to see. Privilege, power, oppression, and domination operate in complex and insidious ways, impacting groups and individuals. And yet, these forces that affect our lives so deeply seem to at once operate in plain sight and lurk in the shadows, making them difficult to discern. Like water to a fish, environments are nearly impossible to perceive when we are immersed in them. This book attempts to expose our environments. With engaging and powerful writing, the contributors share their personal stories as a means of connecting the personal and the public. This volume applies an intersectional perspective to explore how race, class, gender, sexuality, education, and ableness converge, creating the basis for privilege and oppression. Privilege Through the Looking-Glass encourages readers to engage in self and social reflection, and can be used in a range of courses in sociology, social work, communication, education, gender studies, and African American studies. Each chapter includes discussion questions and/or activities for further engagement\"--Publisher description.
Book
Silica colloidal crystals as a gel media replacement
Rigid pore networks formed using nonporous silica particles were used as an alternative separation medium for gel based electrophoretic techniques to enable high-throughput protein separations by reducing separation lengths. The miniaturization of isoelectric focusing to a length as short as 5 mm, with higher resolution than a 100-mm IPG strip, is shown for microchannels packed with chemically modified silica particles. Isoelectric focusing of prostate specific antigen revealed more charge variants in a 10-mm channel than a commercial gel strip with a 100-mm long immobilized pH gradient, with each having the same electric field of 800 V·cm-1 and pH gradient of 3–10. Shortening the channel to 5 mm gave significant drift and compression of the pH gradient. Despite this, comparable resolution for the 5- and 10-mm channels was obtainable in under 10 minutes for the charge variants of prostate specific antigen when a shallower pH gradient of 6 – 8 and a higher field of 1600 V·cm-1 were used for the 5 mm channel length. Size based separations of proteins in silica colloidal crystals in separation lengths of less than 5 mm is characterized for particle diameters of nominally 350 and 500 nm. A model is developed that relates the reduced electrophoretic mobility to the experimentally measurable porosity. The model fits the data with no adjustable parameters for the case of silica colloidal crystals packed in capillaries, for which independent measurements of the pore radii were made from flow data. Band broadening rapidly increases as the pore radius approaches the protein radius, indicating that the main contribution to broadening is the spatial heterogeneity of the pore radius. These combined results support the notion that rigid pore networks formed using nonporous silica can be used as an alternative medium for gel based techniques, and facilitates the design of new separations that would benefit from miniaturization.
Dissertation
Measurement of the distribution of \\(^207\\)Bi depositions on calibration sources for SuperNEMO
The SuperNEMO experiment will search for neutrinoless double-beta decay (\\(0\\)), and study the Standard-Model double-beta decay process (\\(2\\)). The SuperNEMO technology can measure the energy of each of the electrons produced in a double-beta (\\(\\)) decay, and can reconstruct the topology of their individual tracks. The study of the double-beta decay spectrum requires very accurate energy calibration to be carried out periodically. The SuperNEMO Demonstrator Module will be calibrated using 42 calibration sources, each consisting of a droplet of \\(^207\\)Bi within a frame assembly. The quality of these sources, which depends upon the entire \\(^207\\)Bi droplet being contained within the frame, is key for correctly calibrating SuperNEMO's energy response. In this paper, we present a novel method for precisely measuring the exact geometry of the deposition of \\(^207\\)Bi droplets within the frames, using Timepix pixel detectors. We studied 49 different sources and selected 42 high-quality sources with the most central source positioning.
Paper
Formation and dissociation of M₁ muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules
G-protein-coupled receptors (GPCRs) are the largest family of transmembrane signaling proteins in the human genome. Events in the GPCR signaling cascade have been well characterized, but the receptor composition and its membrane distribution are still generally unknown. Although there is evidence that some members of the GPCR superfamily exist as constitutive dimers or higher oligomers, interpretation of the results has been disputed, and recent studies indicate that monomeric GPCRs may also be functional. Because there is controversy within the field, to address the issue we have used total internal reflection fluorescence microscopy (TIRFM) in living cells to visualize thousands of individual molecules of a model GPCR, the M₁ muscarinic acetylcholine receptor. By tracking the position of individual receptors over time, their mobility, clustering, and dimerization kinetics could be directly determined with a resolution of approximately 30 ms and approximately 20 nm. In isolated CHO cells, receptors are randomly distributed over the plasma membrane. At any given time, approximately 30% of the receptor molecules exist as dimers, and we found no evidence for higher oligomers. Two-color TIRFM established the dynamic nature of dimer formation with M₁ receptors undergoing interconversion between monomers and dimers on the timescale of seconds.
Journal Article
Increasing Recovery and Reproducibility of Acidic Peptides in RPLC-based Assays by Reducing Analyte-Surface Interactions
Metal-ion mediated adsorption of sensitive analytes in LC-based assays can negatively impact data quality and assay robustness. Waters[TM] ACQUITY\" PREMIER Columns with MaxPeak[TM] HPS Technology are designed to minimize analyte-surface interactions while improving reproducibility, peak shape, and recovery of metal-surface sensitive analytes.
Magazine Article