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Proximity-dependent biotinylation (BioID) is a readily accessible method for identifying protein associations that occur in living cells. Fusion of a promiscuous biotin ligase to a bait protein for expression in live cells enables covalent biotin labeling, and thus identification, of proteins proximate to the bait. Here we used BioID to probe the organization of the nuclear pore complex, a large structure that regulates molecular transport between the nucleus and cytoplasm. These studies enhance our understanding of major subcomplexes within the nuclear pore complex and demonstrate the utility of BioID for studying the organization of large protein assemblies. Additionally, we have measured the labeling radius of BioID, thus enabling the rational application of this method and more meaningful data interpretation. Proximity-dependent biotin identification (BioID) is a method for identifying protein associations that occur in vivo. By fusing a promiscuous biotin ligase to a protein of interest expressed in living cells, BioID permits the labeling of proximate proteins during a defined labeling period. In this study we used BioID to study the human nuclear pore complex (NPC), one of the largest macromolecular assemblies in eukaryotes. Anchored within the nuclear envelope, NPCs mediate the nucleocytoplasmic trafficking of numerous cellular components. We applied BioID to constituents of the Nup107–160 complex and the Nup93 complex, two conserved NPC subcomplexes. A strikingly different set of NPC constituents was detected depending on the position of these BioID-fusion proteins within the NPC. By applying BioID to several constituents located throughout the extremely stable Nup107–160 subcomplex, we refined our understanding of this highly conserved subcomplex, in part by demonstrating a direct interaction of Nup43 with Nup85. Furthermore, by using the extremely stable Nup107–160 structure as a molecular ruler, we defined the practical labeling radius of BioID. These studies further our understanding of human NPC organization and demonstrate that BioID is a valuable tool for exploring the constituency and organization of large protein assemblies in living cells.

Protected areas (PAs) play a vital role in biodiversity conservation. Their role and importance in promoting sustainable livelihoods through community-based programs and ecotourism ventures in surrounding communities of PAs are unequivocally supported. However, human–wildlife conflicts (HWCs) are a common problem in PAs that threaten long-term conservation goals. Often these HWCs are exacerbated due to a lack of effective communication among community members, or their ability to report those HWCs via networking with relevant authorities. This study aims to evaluate the communication networks of buffer zone residents of Bardiya National Park, Nepal. In addition, the factors affecting their network ties and their perceived level of support for their respective ties are examined. The findings show that residents maintain a high density of ties with parents and neighbors to discuss HWCs; these ties were also perceived to be the most supportive ties. Surprisingly, the Department of National Park and Wildlife Conservation is one of the least used and least supportive ties, while user committee are the least used but the most supportive ties. Our study contributes to a notably debated body of literature, but an underexplored field of social network analysis on HWCs in PAs.

This study highlights ongoing issues in protected area (PA) tourism and presents management suggestions for PAs, given the growing popularity of PA tourism. This study takes a conceptual approach to discuss the ongoing issues within, and the sustainable future of, PAs. The expansion of PAs is a biodiversity conservation strategy. As PAs expand globally to promote conservation, new opportunities for ecotourism development will also evolve, further contributing to the challenge of balancing conservation and tourism. As many PAs operate without management plans, the development of regulations to foster sustainability is necessary, which is even more important now that the COVID-19 pandemic has disrupted PA tourism, challenging PAs across the globe. This study provides an overview of PA tourism, discusses ongoing issues, and offers strategies for managerial improvement. Given the substantial growth of PA tourism, the relationship between PAs and the Sustainable Development Goals (SDGs) should be explored further.

Mechanical integration of the nucleus with the extracellular matrix (ECM) is established by linkage between the cytoskeleton and the nucleus. This integration is hypothesized to mediate sensing of ECM rigidity, but parsing the function of nucleus-cytoskeleton linkage from other mechanisms has remained a central challenge. Here we took advantage of the fact that the LINC (linker of nucleoskeleton and cytoskeleton) complex is a known molecular linker of the nucleus to the cytoskeleton, and asked how it regulates the sensitivity of genome-wide transcription to substratum rigidity. We show that gene mechanosensitivity is preserved after LINC disruption, but reversed in direction. Combined with myosin inhibition studies, we identify genes that depend on nuclear tension for their regulation. We also show that LINC disruption does not attenuate nuclear shape sensitivity to substrate rigidity. Our results show for the first time that the LINC complex facilitates mechano-regulation of expression across the genome.

This study examines effects of weather, temporal factors, and gasoline price on outdoor recreation participation by using a time series model. We obtained more than 5 years of daily outdoor recreation visitation data by using infrared mechanical counters on a section of the Florida National Scenic Trail (FNST). Results showed that days with daily maximum temperatures of 16–22 °C brought the largest number of visitors, which suggests this is the most comfortable range of daily maximum temperatures to recreate on the FNST. Daily maximum temperatures below 6 °C and above 31 °C and heat index values above 38 °C brought significantly lower visitor numbers, suggesting these values are temperature thresholds for this region in a recreation context. A seasonal autoregressive integrated moving average model showed significant negative effects of temperature, relative humidity, cold snaps, and gasoline price and a positive effect of weekends and public holidays on recreational visitations to this trail. Days with heavy rainfall (> 2.54 cm) or a high heat index (≥ 35 °C) were likely to negatively affect recreation participation not only on the same day, but also on the next normal weather day. These findings imply that managers of facilities that need staffing and other resources should expect to receive fewer visitors on days following adverse weather conditions, even if that day has normal weather conditions.

Microentrepreneurship has always been an important driving force of the tourism industry. However, until recently, this sector was mostly invisible and understudied. Microentrepreneurs are now becoming influential stakeholders due to new information technologies that make their offerings easily accessible to a broader clientele and render their economic activity more transparent and taxable. There is a growing consensus that tourism microentrepreneurs can make destinations more competitive and equitable. Accordingly, there has been a surge of scholarship on tourism microentrepreneurship to inform strategies and policies to fuel microentrepreneurial development and its integration with the formal tourism sector. The purpose of this conceptual article is threefold: first, to discuss the definition of tourism microentrepreneurship and commonly used theoretical conceptualizations, as well as the evolution of research on tourism microentrepreneurship; second, to identify research gaps in the existing literature and propose avenues for future research; third, to serve as an introduction to a Special Section on Tourism Microentrepreneurship. In addition, we offer a set of practical recommendations for destination managers and supporting organizations to develop and nurture networks of microentrepreneurs, and to identify suitable and rewarding microentrepreneurial opportunities in the tourism business ecosystem.

This study examines the degree of macroeconomic recovery of the Nepal tourism industry after a natural disaster using the autoregressive integrated moving average model (ARIMA). The study investigated the case of Nepal’s earthquakes in 2015 and examined the impact of the earthquakes on tourism inflows and GDP using time series data from 1990 to 2018. The results show that the increasing trend in the number of tourists changes in the post-earthquake period. In particular, the excess in tourist demand by age and purpose of visits after the earthquake indicates natural disaster as a potential reason for a tourism demand boost, often described as dark tourism in literature. This research shows the process of a heritage tourist destination assessing macroeconomic recovery from a natural disaster and fills the gap in the literature regarding purpose-based tourism demand and a link between dark tourism and disaster recovery on a heritage tourism destination.

This article analyzes the relationship between forest resources, refugees, and the host population. The findings of the research suggest that the host population are heavily dependent on the local forest for their daily needs such as fuelwood, timber, grazing area, fodder for domestic animals, foods, and medicine in addition to cultural and esthetic needs. The forest has also been relied upon for agricultural needs such as manufacture of agricultural tools, maintenance of irrigation water systems, erosion control, and fertilizer needs. The forest was under a sustained demand as any other Terai forest of Nepal. After the arrival of refugees in 1992, the demand for forest resources increased substantially. Initially, the construction of the refugee camps decreased the total forest area and also required some felling of trees. More significantly, the refugees themselves became active users of the forest resource, which generated extra pressure on the forest and created scarcity of forest resources. Before the arrival of the refugees, forest management and monitoring of illegal use of the forest resources were carried out by the government through its local forester office. The local residents were active users of the forest resources, but were passive in managing and maintaining the forest resource. However, competition from the refugees instilled a desire in the local population to safeguard and protect the dwindling resource against the external threat by creating the Humse Dumse Community Forest.

Agritourism has grown significantly worldwide in recent years, even during the COVID-19 pandemic when faced with various challenges. To understand the reasons behind the rapid growth and be better prepared for future crises, this study interviewed 22 agritourism businesses in Texas (USA) regarding the influence of the pandemic and their adaptation strategies. Results indicated that COVID accentuated the entrepreneurial self-efficacy of agritourism business owners, which is key to their growth during the pandemic while highlighting the resource-dependent nature of agritourism businesses. Despite lacking governmental policy support at all levels, agritourism businesses thrived during the pandemic through their creative adaptations. This study provides valuable implications for sustainable agritourism development.

This study examined the potential for developing community-based homestays in Nigeria. Community-based homestays are a sustainable form of tourism that has the capacity to promote the Sustainable Development Goals (SDGs). With its abundant natural and cultural resources, Nigeria has not yet fully utilized these potentials for community-based homestays, which could serve as a strategy for the sustainable development of local communities facing livelihood challenges. Stakeholder theory serves as the theoretical foundation for this study, particularly in its application to a nascent concept. We proposed a framework for effective tourism development in Nigeria, focusing on acceptability, feasibility, and potential outcomes. Qualitative research was conducted through focus group discussions (FDGs) with purposively selected tourism stakeholders, including academia, tour operators, NGOs, and local communities, and further analyzed thematically. The study emphasizes that embracing stakeholder theory rather than relying on a top-down approach is crucial for effectively addressing the varied concerns of key participants before launching community-based homestay tourism. This approach seeks to maximize benefits while minimizing challenges. The result shows that developing community-based homestay tourism in Nigeria is achievable if all stakeholders work together to create supportive tourism policies and agree on a collaborative management framework for the initiative. Furthermore, it is essential to consider the needs of residents by including them in the homestay management committee. Local communities should receive sufficient funding, and effective marketing strategies must be put in place to promote the initiative. To ensure acceptance of homestay tourism among local communities, it is vital to communicate its benefits to potential operators, respect local cultures, and guarantee that economic gains are shared fairly.