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The preservative effects of low temperature on biological materials have been long recognised, and cryopreservation is now widely used in biomedicine, including in organ transplantation, regenerative medicine and drug discovery. The lack of organs for transplantation constitutes a major medical challenge, stemming largely from the inability to preserve donated organs until a suitable recipient is found. Here, we review the latest cryopreservation methods and applications. We describe the main challenges—scaling up to large volumes and complex tissues, preventing ice formation and mitigating cryoprotectant toxicity—discuss advantages and disadvantages of current methods and outline prospects for the future of the field.

Despite the increasing number of studies examining the effects of mindfulness interventions on symptoms associated with Bipolar Disorder (BD), the effectiveness of this type of interventions remains unclear. The aim of the present systematic review was to (i) critically review all available evidence on Mindfulness Based Cognitive Therapy (MBCT) as a form of intervention for BD; (ii) discuss clinical implications of MBCT in treating patients with BD; and (iii) provide a direction for future research. The review presents findings from 13 studies (N = 429) that fulfilled the following selection criteria: (i) included BD patients; (ii) presented results separately for BD patients and control groups (where a control group was available); (iii) implemented MBCT intervention; (iv) were published in English; (v) were published in a peer reviewed journal; and (vi) reported results for adult participants. Although derived from a relatively small number of studies, results from the present review suggest that MBCT is a promising treatment in BD in conjunction with pharmacotherapy. MBCT in BD is associated with improvements in cognitive functioning and emotional regulation, reduction in symptoms of anxiety depression and mania symptoms (when participants had residual manic symptoms prior to MBCT). These, treatment gains were maintained at 12 month follow up when mindfulness was practiced for at least 3 days per week or booster sessions were included. Additionally, the present review outlined some limitations of the current literature on MBCT interventions in BD, including small study sample sizes, lack of active control groups and idiosyncratic modifications to the MBCT intervention across studies. Suggestions for future research included focusing on factors underlying treatment adherence and understanding possible adverse effects of MBCT, which could be of crucial clinical importance.

Stem cells are undifferentiated cells that are present in the embryonic, fetal, and adult stages of life and give rise to differentiated cells that make up the building blocks of tissue and organs. Due to their unlimited source and high differentiation potential, stem cells are considered as potentially new therapeutic agents for the treatment of infertility. Stem cells could be stimulated in vitro to develop various numbers of specialized cells including male and female gametes suggesting their potential use in reproductive medicine. During past few years a considerable progress in the derivation of male germ cells from pluripotent stem cells has been made. In addition, stem cell-based strategies for ovarian regeneration and oocyte production have been proposed as future clinical therapies for treating infertility in women. In this review, we summarized current knowledge and present future perspectives and challenges regarding the use of stem cells in reproductive medicine.

Inland waterway transport represent environmentally friendly transport mode accompanied with other potential benefits, such as increased transport safety and increased cost savings. In order to encourage further development of inland waterway transport in this paper focus is set on the identification of the barriers that limit and prevent development of inland waterway transport. More specifically, emphasis is placed on the barriers that are preventing the cargo shifting from land to waterway transport system. Research conducted in case of Republic of Serbia investigates several barriers which are classified in four main categories: goods, logistics, infrastructure, framework conditions. Framework conditions barriers are further subdivided and classified in four subcategories: political/legal, environment, economy, technology. Results indicate that the most significant negative influence on the further development of the inland waterway transport in Serbia comes from the political/legal barriers. This result implies that the government need to pay more attention to development of inland waterway transport, as well as to make more effort to enhance the cooperation with stakeholders.

One of the basic problems of sustainability in modern society is the reduction of waste, particularly when it comes to food. Food waste has negative impacts on different dimensions of sustainability: social (hunger), economic (resource costs), and environmental (resource consumption and waste generation). This paper focuses on waste reduction through improving the inventory management system in the dairy distribution chain by the application of modern information and communication technologies (ICT). The approach is tested and verified in a case study by application of simulation modelling. Two inventory management models are created, and their impact on waste in the distribution part of the supply chain is examined. Model 1 represents the current dairy inventory management system in the supply of retail stores. Model 2 is based on a higher level of information connectivity between participants (RFID product labelling and the appropriate level of information technology), enabling automatic product ordering and changes in inventory management policy. The obtained results confirm that coordinated inventory management, supported by the application of modern ICT, can significantly contribute to the improvement of the sustainability of the food supply chain, and provide an exact quantification of the given contribution in the case of the dairy industry.

The largest part of food sales is managed by large food supply chains. However, an alternative system of food distribution focuses on locally produced and sold food that has gotten great attention in the last two decades. The challenges of those new systems, called short food supply chains (SFSC), represent tough market competitions, high distribution and logistics costs, small shipment sizes and so forth. Hence, the SFSC requires corresponding solutions in food distribution that are aligned with the contemporary logistics trends, sustainability and aspects of the new digital era. Using specially developed methodology, based on two different conceptual models, we showed how the SFSC could be designed from the aspects of innovative logistics modes and contemporary information and communication technologies, with the final aim to outline and evaluate different food distribution scenarios towards greater sustainability. The first conceptual model was aimed at the creation of innovative forms of SFSC, in which business process modelling was used in order to design and explore the given situation more thoroughly. For the purposes of conducting a comparative assessment of the distribution models developed in the previous part, the second conceptual model is developed. By using a qualitative approach, this is how the major advantages and challenges of practical implementations in creating sustainable distribution solutions are stated for each scenario.

Purpose: The viability of companies in today’s turbulent and dynamic market depends on their ability to change and develop together with the market. Primarily under the influence of technological innovations and new customer demands, the logistics sector is under great pressure of change. The focus of this paper is on the functioning of the distribution hub as an important node in the distribution network and the operational processes it implements. The aim of the paper is to investigate different variants of the development of logistics processes in the distribution hub and thus help decision-makers in deciding on the level and performance of potential technological improvement. Methodology: The research was conducted in a real distribution hub using a simulation approach. In particular, discrete event simulation (DES) was used, which allows supporting the dynamics of logistics processes, examining the impact of random variables on processes and determining process performance in different scenarios. Two models were formed in the paper: the As-Is model that imitates the existing processes in the distribution hub, and the To-Be model that assumes a higher technological level of the process. Results: Findings from this paper have two practical implementations. The first one is to use As-Is models as an insight into the current situation in the distribution hub. The second implementation is to use a To-Be model as a decision support system for evaluating different solutions in the execution of logistics processes. Conclusion: The obtained research results provide an opportunity to examine different ways of the functioning of processes in the distribution hub before making changes in the real system, which is especially important for managers of such systems.

Sustainable development, urban planning, mobility, and transport planning, integrated within the context of sustainable urban mobility, have been central themes in both scientific and applied spheres over the past few decades. In port cities, it becomes particularly essential to tackle sustainability issues given the pollution and noise emanating from ships and other port-related activities. To meet mobility and transportation sustainability needs in the port area, a port should implement measures aligned with a sustainable urban mobility planning (SUMP) approach. However, many ports have thus far achieved limited results in this direction due to the absence of an approach to defining sustainable mobility solutions based on the SUMP approach for an urban area associated with the given port. The overall aim of this paper is to support the development of territorial SUMP for port areas by proposing a methodology that identifies and prioritizes sustainable mobility solutions tailored to a specific port area. The proposed methodology is applied in the Port of Bar (Montenegro) through an appropriate case study. In this case study, the methodological steps are systematically followed, resulting in the practical implementation of the selected mobility solution: the use of a hybrid bus for internal employee transportation within the port area. The undertaken case study underscores the simplicity, practical applicability, and adaptability of the proposed methodology.

The corneal epithelial cells are constantly replaced by the stem cells located at the limbus, the peripheral edge of the cornea, therefore known as limbal stem cells (LSCs). LSCs can be destroyed by numerous factors which results in the condition called limbal stem cell deficiency (LSCD). Ex vivo expansion of LSCs is a well-established technique used successfully to cure patients with LSCD. Therapeutic use of LSCs must be performed in compliance with good manufacturing practice (GMP) as a quality assurance system. However, traditional culture media for ex vivo expansion of LSCs contains a number of ingredients derived from animal sources which may compromise its safety profile for human transplantation. The first aim of the study was to define new GMP grade medium for cultivation and maintenance of LSCs in vitro. Formulation of new GMP compliant media resulted in equal growth to non-GMP grade media. Strick regulations for cell therapy promote centralization of culture units, therefore definition of reliable and practical transportation strategies is vitally important. The second aim of this study was to optimise the transport conditions for limbal biopsies (LBs) and cultured limbal epithelial cells (LECs). Transport of LBs at room temperature proved to be significantly superior to 4°C transport. We also showed that cultured LECs may be stored in serumfree media and transported up to 7 days at 23°C without any negative effect on cell number, viability, colony forming efficiency or gene expression profile. Due to the absence of specific LSC markers, identification and isolation of putative LSCs is a complicated task. The third and final aim of this study was to identify novel cell surface markers for LSCs. We reported herein the identification of a new cell surface marker for LSCs (CD200) as well as a cell surface marker for proliferating progenitor cells (CD109).

The preservative effects of low temperature on biological materials have been long recognised, and cryopreservation is now widely used in biomedicine, including in organ transplantation, regenerative medicine and drug discovery. The lack of organs for transplantation constitutes a major medical challenge, stemming largely from the inability to preserve donated organs until a suitable recipient is found. Here, we review the latest cryopreservation methods and applications. We describe the main challenges--scaling up to large volumes and complex tissues, preventing ice formation and mitigating cryoprotectant toxicity--discuss advantages and disadvantages of current methods and outline prospects for the future of the field.