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Fetal growth restriction (FGR) the leading cause of perinatal mortality and morbidity is highly related to abnormal placental development, and placentas from FGR pregnancies are often characterized by increased inflammation. However, the mechanisms of FGR-associated inflammation are far from being understood. NLRP7, a member of a family of receptors involved in the innate immune responses, has been shown to be associated with gestational trophoblastic diseases. Here, we characterized the expression and the functional role of NLRP7 in the placenta and investigated its involvement in the pathogenesis of FGR. We used primary trophoblasts and placental explants that were collected during early pregnancy, and established trophoblast-derived cell lines, human placental villi, and serum samples from early pregnancy (n = 38) and from FGR (n = 40) and age-matched controls (n = 32). Our results show that NLRP7 (i) is predominantly expressed in the trophoblasts during the hypoxic period of placental development and its expression is upregulated by hypoxia and (ii) increases trophoblast proliferation ([3H]-thymidine) and controls the precocious differentiation of trophoblasts towards syncytium (syncytin 1 and 2 and β-hCG production and xCELLigence analysis) and towards invasive extravillous trophoblast (2D and 3D cultures). We have also demonstrated that NLRP7 inflammasome activation in trophoblast cells increases IL-1β, but not IL-18 secretion. In relation to the FGR, we demonstrated that major components of NLRP7 inflammasome machinery are increased and that IL-1β but not IL-18 circulating levels are increased in FGR. Altogether, our results identified NLRP7 as a critical placental factor and provided evidence for its deregulation in FGR. NLRP7 inflammasome is abundantly expressed by trophoblast cells. It is regulated by a key parameter of placental development, hypoxia. It controls trophoblast proliferation, migration, and invasion and exhibits anti-apoptotic role. NLRP7 machinery is deregulated in FGR pregnancies.Key messagesNLRP7 inflammasome is abundantly expressed by trophoblast cells.It is regulated by a key parameter of placental development, hypoxia.It controls trophoblast proliferation, migration, and invasion and exhibits anti-apoptotic role.NLRP7 machinery is deregulated in FGR pregnancies.

The Mediterranean endemic seagrass Posidonia oceanica forms beds characterised by a dense leaf canopy and a thick root-rhizome 'matte'. Death of P. oceanica shoots leads to exposure of the underlying matte, which can persist for many years, and is termed 'dead' matte. Traditionally, dead matte has been regarded as a degraded habitat. To test whether this assumption was true, the motile macroinvertebrates of adjacent living (with shoots) and dead (without shoots) matte of P. oceanica were sampled in four different plots located at the same depth (5-6 m) in Mellieha Bay, Malta (central Mediterranean). The total number of species and abundance were significantly higher (ANOVA; P<0.05 and P<0.01, respectively) in the dead matte than in living P. oceanica matte, despite the presence of the foliar canopy in the latter. Multivariate analysis (MDS) clearly showed two main groups of assemblages, corresponding to the two matte types. The amphipods Leptocheirus guttatus and Maera grossimana, and the polychaete Nereis rava contributed most to the dissimilarity between the two different matte types. Several unique properties of the dead matte contributing to the unexpected higher number of species and abundance of motile macroinvertebrates associated with this habitat are discussed. The findings have important implications for the conservation of bare P. oceanica matte, which has been generally viewed as a habitat of low ecological value.[PUBLICATION ABSTRACT]

Aquaculture is an increasingly important food-producing sector, providing protein for human consumption. However, marine aquaculture often struggles for space due to the crowded nature of human activities in many marine coastal areas, and because of limited attention from spatial planning managers. Here, we assess the need for coastal spatial planning, emphasising the establishment of suitable areas for the development of marine aquaculture, termed Allocated Zones for Aquaculture (AZAs), in which aquaculture has secured use and priority over other activities, and where potential adverse environmental impacts and negative interactions with other users are minimised or avoided. We review existing examples of marine aquaculture spatial development worldwide and discuss the proper use of site selection in relation to different legal and regulatory requirements. National or regional authorities in charge of coastal zone management should carry out spatial planning defining optimal sites for aquaculture to promote development of sustainable marine aquaculture and avoid conflict with other users, following a participatory approach and adhering to the principles of ecosystem-based management.

Bonnici, L.; Borg, J.A.; Evans, J.; Lanfranco, S., and Schembri, P.J., 2018. Of rocks and hard places: Comparing biotic assemblages on concrete jetties versus natural rock along a microtidal Mediterranean shore. The species composition and structure of littoral assemblages on concrete jetties were compared with those on natural rocky shores at two localities on the microtidal NE coast of Malta. Algal species richness and total algal biomass were significantly lower on jetties. Faunal species richness and faunal abundance were also lower on jetties, albeit not significantly so. Jetties and natural rocky shores differed in relative species composition. The algae Palisada perforata/Laurencia obtusa and several mobile animals, mostly crustaceans, molluscs, and polychaetes, were more abundant on rocky shores. In particular, the supralittoral gastropod Melarhaphe neritoides had a density ranging between 8 ind./m2 to 98 ind./m2 on rocky shores but was never recorded on jetties. Conversely, the algae Cladophoropsis spp., the barnacle Chthamalus stellatus, and the limpets Patella caerulea and P. ulyssiponensis were more abundant on jetties. The rocky shores of sites with jetties were compared with those of a reference site (Dahlet ix-Xmajjar) that lacked jetties. No significant differences occurred in algal biomass and faunal abundance; however, faunal species richness was significantly higher at the reference site. These results may be linked to a number of abiotic factors inclusive of increased exposure to waves, decreased area for colonisation, and habitat homogeneity. The present case study has implications for coastal zone management.

Fetal growth restriction (FGR) affects up to 5 % of pregnancies worldwide, and trophoblast function plays a significant role on the outcome. An epidemiological study has linked vitamin D deficiency to adverse perinatal outcomes, which include decreased birth weight. The placenta as an important source of vitamin D regulates its metabolism through the vitamin D receptor (VDR), but the mechanism by which VDR regulates trophoblast function is poorly understood. Our study aimed at determining placental VDR expression in FGR and gestation-matched control (GMC) pregnancies and identifying the actions of VDR in trophoblast differentiation and apoptosis. Placentae were collected from a well-defined cohort of idiopathic FGR and GMC pregnancies. VDR mRNA and protein expressions were determined by PCR, immunohistochemistry and immunoblotting, while functional consequences of VDR inactivation in vitro were determined on BeWo cells by determining changes in differentiation, attachment and apoptosis. Significant decreases in VDR mRNA expression ( p  = 0.0005) and protein expression ( p  = 0.0003) were observed in the FGR samples, while VDR inactivation, which showed markers for differentiation, cell attachment and apoptosis, was significantly increased. Thus, decreased placental VDR may contribute to uncontrolled premature differentiation and apoptosis of trophoblasts that are characteristics of idiopathic FGR pregnancies. Key message Fetal growth restriction (FGR) affects up to 5 % of all pregnancies worldwide. FGR is the second highest cause of perinatal mortality and morbidity. The placenta plays a pivotal role in vitamin D metabolism during pregnancy. Vitamin D deficiency is associated with adverse pregnancy outcomes. Placental vitamin D receptor expression is decreased in FGR.

Marine reserves are assumed to protect a wide range of species from deleterious effects stemming from exploitation. However, some species, due to their ecological characteristics, may not respond positively to protection. Very little is known about the effects of life history and ecological traits (e.g., mobility, growth, and habitat) on responses of fish species to marine reserves. Using 40 data sets from 12 European marine reserves, we show that there is significant variation in the response of different species of fish to protection and that this heterogeneity can be explained, in part, by differences in their traits. Densities of targeted size-classes of commercial species were greater in protected than unprotected areas. This effect of protection increased as the maximum body size of the targeted species increased, and it was greater for species that were not obligate schoolers. However, contrary to previous theoretical findings, even mobile species with wide home ranges benefited from protection: the effect of protection was at least as strong for mobile species as it was for sedentary ones. Noncommercial bycatch and unexploited species rarely responded to protection, and when they did (in the case of unexploited bentho-pelagic species), they exhibited the opposite response: their densities were lower inside reserves. The use of marine reserves for marine conservation and fisheries management implies that they should ensure protection for a wide range of species with different life-history and ecological traits. Our results suggest this is not the case, and instead that effects vary with economic value, body size, habitat, depth range, and schooling behavior.

Background: Diagnosis of genital Chlamydia trachomatis infection in women traditionally requires a speculum examination to collect endocervical cells, followed by cell culture. This method is time consuming, requires stringent transport conditions, and is technically demanding. Goals: To compare tampons as a patient-administered collection method followed by detection with polymerase chain reaction (PCR) with the traditional endocervical swab culture followed by cell culture detection. Study Design: At the emergency department of a hospital for obstetrics and gynecology, 1,000 consecutive women with symptoms suggestive of infection with trachomatis were tested for trachomatis infection by PCR on both tampon (PCR-T) and swab (PCR-S) specimen and by culture of the swab specimen. Results: Seventeen PCR-T and 16 PCR-S specimens were positive; 16 endocervical specimens were positive by culture, and 14 of the endocervical samples were positive by the three methods. Sixty-one PCR-S samples were inadequate as shown by the lack of amplification of the β-globin gene segment, indicating poor collection of specimens by endocervical swab for chlamydial testing. Conclusions: Tampon specimens collected for PCR detection provided an easy and sensitive method of detection of and subsequent stringent transport requirements of culture.

OBJECTIVE--To identify the prevalence of HPV DNA using the polymerase chain reaction (PCR) in neonatal foreskin and cervical specimens obtained at necropsy. MATERIALS--Foreskin and cervical specimens were obtained from consecutive neonates who had autopsies performed at The Royal Women's Hospital, Melbourne, from June 1991 to February 1992. Specimens were analysed for HPV DNA using the polymerase chain reaction and the L1 consensus primers and generic probes. RESULTS--Specimens were obtained from 98 neonates, 52 males and 46 female. The mean gestational age of the neonates was 29 weeks (range 20-42). Eighty neonates died in utero, three during labour and 15 following delivery. Ninety four were delivered vaginally whilst four were delivered by caesarean section. Samples were collected a mean of 20 hours (range 2-48) from the time of delivery. In 30 cases there was evidence of autolytic change while in the remaining cases, the histology was well preserved. No evidence of HPV DNA was found in any of the samples using the L1 general primers (95% confidence interval 0-3.6%). Recent cervical cytology was available on 70 of the infant's mothers. Six had cytological evidence of HPV infection while the remainder were normal. CONCLUSIONS--HPV DNA is uncommonly detected (by PCR) in foreskin and cervical specimens obtained from neonates.

OBJECTIVE--To study the prevalence of human papillomavirus (HPV) infection, using several different hybridisation techniques, in men whose female sexual partners had cervical HPV and/or cervical intraepithelial neoplasia (CIN). METHODS--The male genital area was examined colposcopically and areas suspicious of HPV changes were biopsied. Each biopsy was subjected to histological examination and HPV DNA analysis by conventional DNA analysis such as Southern, reverse and dot blot as well as with polymerase chain reaction (PCR). RESULTS--Colposcopic examination of men showed 133 to be normal whilst 82 (38%) had clinical or subclinical lesions. Of 55 colposcopically directed biopsies from the male lesions taken, detection of HPV DNA by hybridisation with conventional techniques and by PCR showed HPV DNA in 29 (53%) and 47 (85%) of biopsies respectively. Overall HPV types 6/11 were the predominant types. In 18 (33%) biopsies positive by PCR, multiple types were found. CONCLUSION--HPV DNA was present in the majority of biopsy specimens taken, with HPV 6/11 being the predominant type. Among methods for HPV DNA detection, PCR was the most sensitive and useful technique.