Catalogue Search | MBRL
Are you sure you want to remove the book from the shelf?
{{itemTitle}}
10
result(s) for
"Boss, Tyler"
Sort by:
Home
\"Discover the secret origins of the Triceratons: how they began on Earth, what that means for their return, and how they successfully rebelled against their Utrom captors! Plus, the Turtles fight to save their city, but become trapped in tight quarters when NYC is evacuated. Will they find a way to freedom without driving each other crazy? Then, Raphael and Alopex go undercover at Null Industries where they find more than they bargained for, including very angry mutants!\" --Amazon.com.
Book
Ensuring a Mold's Thermal Integrity
With ever-increasing energy and materials costs, manufacturers are more motivated to improve productivity by streamlining processes, dramatically cutting human resources and budgets and optimizing production processes. For example, shops pay more attention to a molds thermal dynamics during design, molding and preventative maintenance (PM) to reduce cycle time, decrease energy consumption, improve plastic part quality and increase yield. Manufacturers have optimized hot runner systems, gating and finite element method (FEM) simulations to control better fill and cycle times. However, what is rarely discussed is cooling. The cooling cycle can be as much as 70% of a part run cycle, so thermal management to reduce overall cycle times is critical for process optimization. Lower cycle times and lower scrap rates equate to higher part production.
Magazine Article
Comparative genomics of the plant-growth promoting bacterium Sphingobium sp. strain AEW4 isolated from the rhizosphere of the beachgrass Ammophila breviligulata
Background
The genus
Sphingobium
within the class Alpha-proteobacteria contains a small number of plant-growth promoting rhizobacteria (PGPR), although it is mostly comprised of organisms that play an important role in biodegradation and bioremediation in sediments and sandy soils. A
Sphingobium
sp. isolate was obtained from the rhizosphere of the beachgrass
Ammophila breviligulata
with a variety of plant growth-promoting properties and designated as
Sphingobium
sp. strain AEW4.
Results
Analysis of the 16S rRNA gene as well as full genome nucleotide and amino acid identities revealed that this isolate is most similar to
Sphingobium xenophagum
and
Sphingobium hydrophobicum
. Comparative genomics analyses indicate that the genome of strain AEW4 contains unique features that explain its relationship with a plant host as a PGPR, including pathways involved in monosaccharide utilization, fermentation pathways, iron sequestration, and resistance to osmotic stress. Many of these unique features are not broadly distributed across the genus. In addition, pathways involved in the metabolism of salicylate and catechol, phenyl acetate degradation, and DNA repair were also identified in this organism but not in most closely related organisms.
Conclusion
The genome of
Sphingobium
sp. strain AEW4 contains a number of distinctive features that are crucial to explain its role as a plant-growth promoting rhizobacterium, and comparative genomics analyses support its classification as a relevant
Sphingobium
strain involved in plant growth promotion of beachgrass and other plants.
Journal Article
A Binder Jet Printed, Stainless Steel Preconcentrator as an In-Line Injector of Volatile Organic Compounds
A conventional approach to making miniature or microscale gas chromatography (GC) components relies on silicon as a base material and MEMS fabrication as manufacturing processes. However, these devices often fail in medium-to-high temperature applications due to a lack of robust fluidic interconnects and a high-yield bonding process. This paper explores the feasibility of using metal additive manufacturing (AM), which is also known as metal 3D printing, as an alternative platform to produce small-scale microfluidic devices that can operate at a temperature higher than that which polymers can withstand. Binder jet printing (BJP), one of the metal AM processes, was utilized to make stainless steel (SS) preconcentrators (PCs) with submillimeter internal features. PCs can increase the concentration of gaseous analytes or serve as an inline injector for GC or gas sensor applications. Normally, parts printed by BJP are highly porous and thus often infiltrated with low melting point metal. By adding to SS316 powder sintering additives such as boron nitride (BN), which reduces the liquidus line temperature, we produce near full-density SS PCs at sintering temperatures much lower than the SS melting temperature, and importantly without any measurable shape distortion. Conversely, the SS PC without BN remains porous after the sintering process and unsuitable for fluidic applications. Since the SS parts, unlike Si, are compatible with machining, they can be modified to work with commercial compression fitting. The PC structures as well as the connection with the fitting are leak-free with relatively high operating pressures. A flexible membrane heater along with a resistance-temperature detector is integrated with the SS PCs for thermal desorption. The proof-of-concept experiment demonstrates that the SS PC can preconcentrate and inject 0.6% headspace toluene to enhance the detector’s response.
Journal Article
KSHV miRNAs Decrease Expression of Lytic Genes in Latently Infected PEL and Endothelial Cells by Targeting Host Transcription Factors
Kaposi’s sarcoma-associated herpesvirus (KSHV) microRNAs are encoded in the latency-associated region. Knockdown of KSHV miR-K12-3 and miR-K12-11 increased expression of lytic genes in BC-3 cells, and increased virus production from latently infected BCBL-1 cells. Furthermore, iSLK cells infected with miR-K12-3 and miR-K12-11 deletion mutant viruses displayed increased spontaneous reactivation and were more sensitive to inducers of reactivation than cells infected with wild type KSHV. Predicted binding sites for miR-K12-3 and miR-K12-11 were found in the 3’UTRs of the cellular transcription factors MYB, Ets-1, and C/EBPα, which activate RTA, the KSHV replication and transcription activator. Targeting of MYB by miR-K12-11 was confirmed by cloning the MYB 3’UTR downstream from the luciferase reporter. Knockdown of miR‑K12-11 resulted in increased levels of MYB transcript, and knockdown of miR-K12-3 increased both C/EBPα and Ets-1 transcripts. Thus, miR-K12-11 and miR-K12-3 contribute to maintenance of latency by decreasing RTA expression indirectly, presumably via down‑regulation of MYB, C/EBPα and Ets-1, and possibly other host transcription factors.
Journal Article
Rigid bronchoscopy and Y-stent for severe central malignant airway obstruction as a bridge to surgery requiring general anaesthesia
A female patient in her 30s with a history of asthma presented with a chronic cough, worsening dyspnoea, dysphagia and a painless neck lump. Ultrasound revealed enlarged cervical lymph nodes and chest X-ray showed a large anterior mediastinal mass causing central airway obstruction. CT confirmed the mass partially encasing the superior vena cava. Due to concerns of airway collapse during surgery, interventional pulmonologists placed a silicone Y stent to restore airway patency before induction of anaesthesia for surgical lymph node biopsy. The excisional biopsy confirmed classic Hodgkin’s lymphoma. The patient underwent chemotherapy (ABVD regimen) and had the stent removed after 2 months. She is in remission and continues follow-up.
Journal Article
Oceanography, biogeochemical cycles, and biodiversity in the Central Arctic Ocean: Current state of knowledge and research directions for the Tara Polaris expeditions
Climatic changes in the physical environment modulate biogeochemical cycles, biodiversity, and trophic interactions in the Central Arctic Ocean (CAO). Physical processes and sea-ice conditions are highly seasonal in the CAO and dependent on interactions that occur throughout the evolution of the upper ocean–sea ice–lower atmosphere system. Understanding these seasonal interactions is critical to comprehending and predicting the long-term trends as the CAO moves towards ice-free summers and to informing future policy decisions at the core of ongoing discussions concerning the CAO Fisheries agreement, for example, at the Arctic Council and International Council for Exploration of the Sea working group on the CAO. Here, we review current knowledge of the physical environment, biogeochemical cycles, and biodiversity in the waters of the CAO, identify emerging research questions, and introduce the science plan for the first Tara Polaris drift onboard the Tara Polar Station to advance knowledge and address these questions. Despite increased observational programmes in the CAO over the past years, for example, the Nansen and Amundsen Basin Observational System (NABOS) and Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC), extensive knowledge gaps remain in relation to ocean stratification, sea ice and lightscape, nitrogen fixation and nutrient fluxes, carbon export and transfer, sympagic-pelagic coupling, aerosol production, contaminant transport and transformation, chronobiology, and fish distribution. Further knowledge on overall CAO biodiversity, ecosystem functionality and interannual variability is also critically needed. We describe a way forward to address these knowledge gaps using ice-tethered and profiling instruments coupled with multi-omics, culturing, and imagery approaches deployed from Tara Polar Station during the first of ten Tara Polaris drifts designed to facilitate detection of interannual variability and change over time.
Journal Article
Jump-starting the T cell response in established tumors
Checkpoint blockade only works in 10-20% of patients. Consequently, investigators are testing checkpoint inhibitors in combination with drugs like the class I histone deacetylase inhibitor, entinostat (ENT). Unfortunately, the combination of ENT and checkpoint blockade fared poorly in patients with breast or ovarian cancer, despite promising pre-clinical results. Here we show that ENT enhances CD8
T cell responses by maintaining a progenitor-like population of CD8
T cells that supplies activated effector T cells to tumors for prolonged periods. Surprisingly, the anti-tumor effects of ENT are only experienced when delivered during a narrow window that occurs after T cell activation and before T cell exhaustion-a window that is likely closed in most patients. However, by first \"jump-starting\" the T cell response using an oncolytic virus, the anti-tumor activity of ENT and PD1 blockade is restored. These data establish a general paradigm, independent of tumor type, to rationally manipulate anti-tumor immunity.
Journal Article
Extraction of beam-spin asymmetries from the hard exclusive \\(^+\\) channel off protons in a wide range of kinematics
We have measured beam-spin asymmetries to extract the \\(\\) moment \\(A_LU^\\) from the hard exclusive \\(e p e^ n ^+\\) reaction above the resonance region, for the first time with nearly full coverage from forward to backward angles in the center-of-mass. The \\(A_LU^\\) moment has been measured up to 6.6 GeV\\(^2\\) in \\(-t\\), covering the kinematic regimes of Generalized Parton Distributions (GPD) and baryon-to-meson Transition Distribution Amplitudes (TDA) at the same time. The experimental results in very forward kinematics demonstrate the sensitivity to chiral-odd and chiral-even GPDs. In very backward kinematics where the TDA framework is applicable, we found \\(A_LU^\\) to be negative, while a sign change was observed near 90\\(^\\) in the center-of-mass. The unique results presented in this paper will provide critical constraints to establish reaction mechanisms that can help to further develop the GPD and TDA frameworks.
Paper