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"Brown, Greg"
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Determinants of International Buyout Investments
Using a proprietary data set on international private equity activity, we study the determinants of buyout investments across 61 countries and 19 industries over the period of 1990 to 2017. We find that countries with cyclically strong economies, more active stock and credit markets, and better rule of law experience more buyout activity. Countries also receive more buyout capital following investor protection and contract enforcement reforms. The set of determinants we identify appear somewhat unique to buyout investments, because other forms of investment such as foreign direct investment, gross capital formation, investments in R&D, and M&A activity do not respond similarly to these factors.
Journal Article
North, south, east, west
Follows the journey of a little bird who flies to the north, south, east, and west to decide which direction she likes best.
Book
Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference
Clustered regularly interspaced short palindromic repeats (CRISPRs) and Cas proteins represent an adaptive microbial immunity system against viruses and plasmids. Cas3 proteins have been proposed to play a key role in the CRISPR mechanism through the direct cleavage of invasive DNA. Here, we show that the Cas3 HD domain protein MJ0384 from
Methanocaldococcus jannaschii
cleaves endonucleolytically and exonucleolytically (3′–5′) single‐stranded DNAs and RNAs, as well as 3′‐flaps, splayed arms, and R‐loops. The degradation of branched DNA substrates by MJ0384 is stimulated by the Cas3 helicase MJ0383 and ATP. The crystal structure of MJ0384 revealed the active site with two bound metal cations and together with site‐directed mutagenesis suggested a catalytic mechanism. Our studies suggest that the Cas3 HD nucleases working together with the Cas3 helicases can completely degrade invasive DNAs through the combination of endo‐ and exonuclease activities.
Cas3 is an essential protein required for CRISPR‐based bacteriophage immunity in bacteria. This study describes the structure, biochemical activity, and catalytic mechanism of Cas3.
Journal Article
Skaar : son of Hulk - the complete collection
Born in fire. Raised by monsters. Destined to smash! On an alien planet shattered by war, no one is stronger than Skaar -- the savage Son of Hulk! But as a warlord and a princess spread chaos through the wastelands, will Skaar save the puny survivors -- or eat them? Skaar seeks the mysterious Old Power, but can even he stop the coming of the Silver Surfer-and Galactus the Devourer? The soothsayers sing: One day, monsters will clash -- the boy will confront the man who abandoned him. When the Son of Hulk seeks vengeance on his father, will Earth be turned into Planet Skaar?
BOOK
Cane toads (Rhinella marina) rely on water access, not drought tolerance, to invade xeric Australian environments
The invasion of habitats with novel environmental challenges may require physiological tolerances not seen in conspecifics from the native range. We used a combination of field and laboratory-based experiments to assess physiological tolerance to limited water access at four sites distributed across the historical invasion path of cane toads (Rhinella marina) in Australia that, from east to west, alternated between mesic and seasonally xeric habitats. Toads from all locations were well hydrated at the time of capture. However, experimental dehydration caused greater mass loss, higher plasma osmolality, and inhibition of lytic ability in toads from xeric compared to mesic locations. These results suggest somewhat surprisingly that toads from xeric environments are physiologically more vulnerable to water loss. In contrast, bactericidal ability was not sensitive to hydric state and was greater in toads from eastern (long-colonized) areas. Similar patterns in lytic ability in hydrated toads and agglutination ability in wild toads suggest that toads along the invasion front face a tradeoff between enhanced dispersal ability and physiological responses to dehydration. The ability of this invasive species to spread into drier environments may be underpinned by a combination of phenotypic plasticity and evolved (heritable) traits.
Journal Article
Using crowdsourced spatial data from Flickr vs. PPGIS for understanding nature's contribution to people in Southern Norway
Crowdsourced data can provide spatially explicit data on the contribution of nature to people. Spatial information is essential for effectively managing the diverse relationships that people have with nature, but the potential and limits of using crowdsourcing data to generate maps for conservation purposes need further research. Passive crowdsourcing tools include social media platforms where photos and user‐generated tags are shared among users, whereas active crowdsourcing, such as public participatory geographic information system (PPGIS), provides an online platform for mapping place attributes such as values, experiences and preferences. In this study, we assess the spatial information gained through using Flickr (a photo sharing platform) and PPGIS (an online mapping platform) platforms for conservation planning to understand differences and similarities on the spatial distribution of values captured by the two platforms, and to identify what environmental and infrastructure variables correlate best with the distribution of values. We test these tools in Southern Norway including protected areas and the surrounding zones. We analysed non‐spatial (using chi‐square and Spearman rank correlation) and spatial (using clustering, Maxent and distribution overlap) data to identify differences between the two datasets and the values represented therein. We found large differences in spatial distribution using these two datasets, with Flickr data concentrated outside the protected areas and near roads, whereas PPGIS provided more fine‐scale data on diverse values in locations inaccessible by roads within the protected areas. Flickr can be used for generating regional scale data of scenic landscapes or routes, but PPGIS performs better for management of nature qualities appreciated by different user groups within protected areas. We discuss the pros and cons of using each data source and when each dataset is more suitable to be used in protected area management.
Journal Article
Levels of Cholesterol in Small LDL Particles Predict Atherosclerosis Progression and Incident CHD in the HDL-Atherosclerosis Treatment Study (HATS)
Test whether angiographically-documented changes in percent stenosis and clinical endpoints (coronary-related deaths, myocardial infarctions, stroke, revascularization for worsening ischemia) in the HDL-Atherosclerosis Treatment Study (HATS) were attributable to specific LDL-subclasses.
Gradient gel electrophoresis of on-study LDL-subclass cholesterol concentrations were measured in 32 placebo, 33 simvastatin-niacin, 38 antioxidant, and 39 simvastatin-niacin & antioxidant treated participants. The prespecified primary end point was the mean change per patient from the initial arteriogram to the final arteriogram in the percent stenosis caused by the most severe lesion in each of the nine proximal coronary segments.
The change in the percent stenosis of the most severe proximal lesions increased in association with higher concentrations of the small LDL subfractions LDL-IIIb (24.2-24.6 nm) and LDL-IVa (23.3-24.1 nm) before (both P = 0.002) and after (P = 0.01 and P = 0.03 respectively) adjustment for treatment group and on-study HDL-cholesterol, LDL-cholesterol, and triglyceride concentrations. The associations appeared specific to lesions with <30% baseline stenosis. When adjusted for age, sex, baseline BMI and cigarette use, the odds for primary clinical endpoints (death from coronary causes, nonfatal myocardial infarction, stroke, or revascularization for worsening ischemia) were significantly greater in subjects with higher on-study LDL-IIIb levels both before (P = 0.01) and after (P = 0.03) adjustment for treatment group and the standard lipid values.
Plasma LDL-IIIb cholesterol concentrations were related to changes in coronary artery stenosis and cardiovascular events in patients with coronary artery disease and low HDL-cholesterol.
ClinicalTrials.gov NCT00000553.
Journal Article
Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families
Metagenomics has made accessible an enormous reserve of global biochemical diversity. To tap into this vast resource of novel enzymes, we have screened over one million clones from metagenome DNA libraries derived from sixteen different environments for carboxylesterase activity and identified 714 positive hits. We have validated the esterase activity of 80 selected genes, which belong to 17 different protein families including unknown and cyclase-like proteins. Three metagenomic enzymes exhibited lipase activity, and seven proteins showed polyester depolymerization activity against polylactic acid and polycaprolactone. Detailed biochemical characterization of four new enzymes revealed their substrate preference, whereas their catalytic residues were identified using site-directed mutagenesis. The crystal structure of the metal-ion dependent esterase MGS0169 from the amidohydrolase superfamily revealed a novel active site with a bound unknown ligand. Thus, activity-centered metagenomics has revealed diverse enzymes and novel families of microbial carboxylesterases, whose activity could not have been predicted using bioinformatics tools.
Journal Article