Explore the vast range of titles available.

Andean glaciers are among the fastest shrinking and largest contributors to sea level rise on Earth. They also represent crucial water resources in many tropical and semi-arid mountain catchments. Yet the magnitude of the recent ice loss is still debated. Here we present Andean glacier mass changes (from 10° N to 56° S) between 2000 and 2018 using time series of digital elevation models derived from ASTER stereo images. The total mass change over this period was −22.9 ± 5.9 Gt yr−1 (−0.72 ± 0.22 m w.e. yr−1 (m w.e., metres of water equivalent)), with the most negative mass balances in the Patagonian Andes (−0.78 ± 0.25 m w.e. yr−1) and the Tropical Andes (−0.42 ± 0.24 m w.e. yr−1), compared to relatively moderate losses (−0.28 ± 0.18 m w.e. yr−1) in the Dry Andes. Subperiod analysis (2000–2009 versus 2009–2018) revealed a steady mass loss in the tropics and south of 45° S. Conversely, a shift from a slightly positive to a strongly negative mass balance was measured between 26 and 45° S. In the latter region, the drastic glacier loss in recent years coincides with the extremely dry conditions since 2010 and partially helped to mitigate the negative hydrological impacts of this severe and sustained drought. These results provide a comprehensive, high-resolution and multidecadal data set of recent Andes-wide glacier mass changes that constitutes a relevant basis for the calibration and validation of hydrological and glaciological models intended to project future glacier changes and their hydrological impacts.Glaciers in the Andes have lost about 23 Gt of mass per year between 2000 and 2018, with the fastest loss in Patagonia, according to time series of digital elevation models that are based on ASTER stereo images.

In Arabidopsis the plasma membrane nitrate transceptor (transporter/receptor) NRT1.1 governs many physiological and developmental responses to nitrate. Alongside facilitating nitrate uptake, NRT1.1 regulates the expression levels of many nitrate assimilation pathway genes, modulates root system architecture, relieves seed dormancy and protects plants from ammonium toxicity. Here, we assess the functional and phenotypic consequences of point mutations in two key residues of NRT1.1 (P492 and T101). We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1 , and its longer-term downregulation, at high nitrate concentrations. We also show that these mutations have differential effects on genome-wide gene expression. Our findings indicate that NRT1.1 activates four separate signalling mechanisms, which have independent structural bases in the protein. In particular, we present evidence to suggest that the phosphorylated and non-phosphorylated forms of NRT1.1 at T101 have distinct signalling functions, and that the nitrate-dependent regulation of root development depends on the phosphorylated form. Our findings add to the evidence that NRT1.1 is able to trigger independent signalling pathways in Arabidopsis in response to different environmental conditions. In Arabidopsis the plasma membrane nitrate transceptor (transporter/receptor) NRT1.1 governs many physiological and developmental responses to nitrate. Point mutations in two key residues of the transceptor differentially affect several of the NRT1.1-dependent responses to nitrate, suggesting that NRT1.1 activates independent signalling pathways.

The degenerative effects of multiple sclerosis at the level of the vascular and neuronal networks in the central nervous system are currently the object of intensive investigation. Preclinical studies have demonstrated the efficacy of mesenchymal stem cell (MSC) therapy in experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis, but the neuropathology of specific lesions in EAE and the effects of MSC treatment are under debate. Because conventional imaging techniques entail protocols that alter the tissues, limiting the reliability of the results, we have used non-invasive X-ray phase-contrast tomography to obtain an unprecedented direct 3D characterization of EAE lesions at micro-to-nano scales, with simultaneous imaging of the vascular and neuronal networks. We reveal EAE-mediated alterations down to the capillary network. Our findings shed light on how the disease and MSC treatment affect the tissues, and promote X-ray phase-contrast tomography as a powerful tool for studying neurovascular diseases and monitoring advanced therapies.

Eutrophication is one of the major threats facing seagrasses, promoting effects in different compartments of the community (e.g. plants, epiphytes, fauna). In this study, we researched how in situ nutrient enrichment modified the consumption rates of Cymodocea nodosa plants during a period of 3 mo, by creating a set of mesocosm feeding choice experiments with the generalist herbivore Paracentrotus lividus. Nutrient enrichment intensified the consumption of C. nodosa leaves by increasing the palatability of their tissues at different levels. At a first level (i.e. the individual plant response), nutritional quality of the tissues increased (i.e. nitrogen content), while both biomechanical (i.e. absolute force-to-tear, F TA, and specific force-to-tear, F TS) and mechanical (i.e. fiber content) traits were reduced. At a second level, the presence of epiphytes coating the leaves increased their nutritional quality without actually increasing their biomechanical resistance, which rendered higher consumption rates. However, the presence of Ulva sp. (a highly palatable macrophyte) reduced the direct consumption of C. nodosa leaves, mainly when coated by epiphytes, a fact fully endorsed by the compensatory feeding theory. Therefore, the nutritional quality of the C. nodosa leaves is a key factor regulating their susceptibility to be consumed, although mechanical and biomechanical factors also gained importance when nutritional quality was low. In addition, the presence of other components of the community (epiphytes and macroalgae) may increase or reduce herbivore pressure, highlighting the complex nature of herbivore–plant interrelationships.

Few glaciological field data are available on glaciers in the Hindu Kush–Karakoram–Himalayan (HKH) region, and remote sensing data are thus critical for glacier studies in this region. The main objectives of this study are to document, using satellite images, the seasonal changes of surface albedo for two Himalayan glaciers, Chhota Shigri Glacier (Himachal Pradesh, India) and Mera Glacier (Everest region, Nepal), and to reconstruct the annual mass balance of these glaciers based on the albedo data. Albedo is retrieved from Moderate Resolution Imaging Spectroradiometer (MODIS) images, and evaluated using ground based measurements. At both sites, we find high coefficients of determination between annual minimum albedo averaged over the glacier (AMAAG) and glacier-wide annual mass balance (Ba) measured with the glaciological method (R2 = 0.75). At Chhota Shigri Glacier, the relation between AMAAG found at the end of the ablation season and Ba suggests that AMAAG can be used as a proxy for the maximum snow line altitude or equilibrium line altitude (ELA) on winter-accumulation-type glaciers in the Himalayas. However, for the summer-accumulation-type Mera Glacier, our approach relied on the hypothesis that ELA information is preserved during the monsoon. At Mera Glacier, cloud obscuration and snow accumulation limits the detection of albedo during the monsoon, but snow redistribution and sublimation in the post-monsoon period allows for the calculation of AMAAG. Reconstructed Ba at Chhota Shigri Glacier agrees with mass balances previously reconstructed using a positive degree-day method. Reconstructed Ba at Mera Glacier is affected by heavy cloud cover during the monsoon, which systematically limited our ability to observe AMAAG at the end of the melting period. In addition, the relation between AMAAG and Ba is constrained over a shorter time period for Mera Glacier (6 years) than for Chhota Shigri Glacier (11 years). Thus the mass balance reconstruction is less robust for Mera Glacier than for Chhota Shigri Glacier. However our method shows promising results and may be used to reconstruct the annual mass balance of glaciers with contrasted seasonal cycles in the western part of the HKH mountain range since the early 2000s when MODIS images became available.

Correlative approaches are a powerful tool in the investigation of biological samples, but require specific preparation procedures to maintain the strength of the employed methods. Here we report the optimization of the embedding protocol of nervous system samples for a correlative synchrotron X-ray computed microtomography (micro-CT) and transmission electron microscopy (TEM) approach. We demonstrate that it is possible to locate, with the micrometric resolution of micro-CT, specific volumes of interest for a further ultrastructural characterization to be performed with TEM. This approach can be applied to samples of different size and morphology up to several cm. Our optimized method represents an invaluable tool for investigating those pathologies in which microscopic alterations are localized in few confined regions, rather than diffused in entire tissues, organs or systems. We present a proof of concept of our method in a mouse model of Globoid Cells Leukodistrophy.

New views of Titan The Huygens probe landed on Titan on 14 January this year, and seven papers published in this issue record the encounter. They describe a world that resembles a primitive Earth, complete with weather systems and geological activity. The ‘Huygens on Titan’ section opens with an overview of the descent and landing and a News and Views piece. Tomasko et al . describe the dry riverbed and drainage channels seen during Huygens' descent, evidence that liquid methane falls as rain or erupts from cryovolcanoes, periodically flooding the surface. This paper includes the images used on the cover to the Huygens section. Niemann et al . measured the abundances of isotopes of argon, nitrogen and carbon in the atmosphere, and conclude that there is no evidence that Titan's methane comes from biological activity. Fulchignoni et al . obtained precise measurements of temperature and pressure from the upper atmosphere right down to the surface. On the way down Huygens recorded evidence for lightning. Zarnecki et al . report that the probe landed on a relatively smooth surface of icy grains with the consistency of wet clay or sand. Isräl et al . report that the aerosols in Titan's clouds have solid cores made from complex organic molecules containing carbon and nitrogen. And Bird et al . found that on average Titan's winds blow in the same direction as the moon rotates, and that close to the surface these winds are very weak, travelling at around walking speed. Aerosols in Titan's atmosphere play an important role in determining its thermal structure 1 , 2 , 3 . They also serve as sinks for organic vapours 4 and can act as condensation nuclei for the formation of clouds 5 , 6 , where the condensation efficiency will depend on the chemical composition of the aerosols 5 , 7 . So far, however, no direct information has been available on the chemical composition of these particles. Here we report an in situ chemical analysis of Titan's aerosols by pyrolysis at 600 °C. Ammonia (NH 3 ) and hydrogen cyanide (HCN) have been identified as the main pyrolysis products. This clearly shows that the aerosol particles include a solid organic refractory core. NH 3 and HCN are gaseous chemical fingerprints of the complex organics that constitute this core, and their presence demonstrates that carbon and nitrogen are in the aerosols.

Seagrasses are marine flowering plants distributed worldwide. They are however threatened, mostly due to the increase of human activities. Seagrasses have the capacity to adapt their morphological, physiological, and mechanical traits to their local conditions. Mechanical traits have been identified as a good tool to investigate a plant‐species capacity to withstand physical forces or disturbances but are still sparsely studied in seagrasses. With this study, we aimed to assess how the mechanical traits of a broadly spread seagrass species vary along a latitudinal gradient in relation to its morphometric plasticity and nutrient status. We found that seagrasses acclimate their mechanical traits in relation to their physiological or morphological traits, both over the growing season and across a latitudinal range: leaves were weaker and thinner in northern areas, particularly at the end of the growing season. Besides the influence of the latitudinal gradient, leaf mechanical strength and stiffness were both strongly affected by their morphometric plasticity. Moreover, we showed that leaves mechanical traits change depending on their nutrient status: leaves were stronger and stiffer in oligotrophic conditions as compared to more eutrophic conditions. Thus, our results imply that, under eutrophication, leaves become weaker and thus more vulnerable to physical forces. This vulnerability is higher in the north at the end of the growing season. The latter is consistent with the more ephemeral character of northern seagrass meadows, in contrast to the more evergreen southern meadows.

Pollen limitation may be an important factor in accelerated decline of sparse or fragmented populations. Little is known whether hydrophilous plants (pollen transport by water) suffer from an Allee effect due to pollen limitation or not. Hydrophilous pollination is a typical trait of marine angiosperms or seagrasses. Although seagrass flowers usually have high pollen production, floral densities are highly variable. We evaluated pollen limitation for intertidal populations of the seagrass Zostera noltei in The Netherlands and found a significant positive relation between flowering spathe density and fruit-set, which was suboptimal at < 1200 flowering spathes m-2 (corresponding to < 600 reproductive shoots m-2). A fragmented population had ˜35 % lower fruit-set at similar reproductive density than a continuous population. 75 % of all European populations studied over a large latitudinal gradient had flowering spathe densities below that required for optimal fruitset, particularly in Southern countries. Literature review of the reproductive output of hydrophilous pollinated plants revealed that seed-or fruit-set of marine hydrophilous plants is generally low, as compared to hydrophilous freshwater and wind-pollinated plants. We conclude that pollen limitation as found in Z. noltei may be a common Allee effect for seagrasses, potentially accelerating decline and impairing recovery even after environmental conditions have improved substantially.

Successful management and restoration of coastal vegetation requires a quantitative process-based understanding of thresholds hampering (re-)establishment of pioneer vegetation. We expected scouring to be important in explaining the disappearance of seedlings and/or small propagules of intertidal plant species, and therefore quantified the dependence of scouring on plant traits (flexibility, size) and physical forcing by current velocity. Flume studies with unidirectional flow revealed that scouring around seedlings increased exponentially with current velocity and according to a power relationship with plant size. Basal stem diameter rather than shoot length controlled scouring volume. Flexible shoots caused far less scouring than stiff shoots, provided that the bending occurred near the sediment surface as was the case forZostera, and not on top of a solid tussock base as we observed forPuccinellia. Therefore, shoot stiffness is likely to strongly affect the chances for initial establishment in hydrodynamically exposed areas. Plant traits such as shoot stiffness are subject to a trade-off between advantages and disadvantages, the outcome of which depends on the physical settings.