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The Eastern Adriatic region, encompassing Croatia and Bosnia and Herzegovina, is considered an important area of natural populations of wild grapevines (Vitis vinifera ssp. sylvestris). The wild grapevine arises in the Eastern Adriatic region in a contact zone of the EU-Mediterranean and the sub-Mediterranean characterized by typical karst relief. This study focuses on the chloroplast DNA (cpDNA) analysis of wild grapevines and the biodiversity of accompanying woody species to better understand the genetic variation of the sylvestris populations of the Eastern Adriatic region and to investigate how this variation fits within today's wild grapevine distribution in the European continent. The allelic variation at nine cpDNA microsatellite loci of wild individuals was used to characterize haplotype diversity in 53 individuals from four population sites. All individuals were grouped into two chlorotypes: A and D, D being the rare haplotype among wild populations on the European continent. In total, 52 woody plant species were identified. However, the studied vegetation structures have been affected by permanent human pressure on natural resources and the preservation status of the collection sites. Based on our results, we conclude that the investigated areas were probably shelter zones for wild grapevine preservation during the unfavorable glaciation era.

Mediterranean forest ecosystems in Croatia are of very high significance because of the ecological functions they provide. This region is highly sensitive to abiotic stresses such as air pollution, high sunlight, and high temperatures alongside dry periods; therefore, it is important to monitor the state of these forest ecosystems and how they respond to these stresses. This study was conducted on trees in situ and focused on the four most important forest species in the Mediterranean region in Croatia: pubescent oak (Quercus pubescens Willd.), holm oak (Quercus ilex L.), Aleppo pine (Pinus halepensis Mill.) and black pine (Pinus nigra J. F. Arnold.). Trees were selected and divided into two groups: trees with defoliation of >25% (defoliated) and trees with defoliation of ≤25% (undefoliated). Leaves and needles were collected from selected trees. Differences in chlorophyll content, hydrogen peroxide content, lipid peroxidation and enzyme activity (superoxide dismutase, catalase, ascorbate peroxidase, non-specific peroxidase), and nutrient content between the defoliated and undefoliated trees of the examined species were determined. The results showed that there were significant differences for all species between the defoliated and undefoliated trees for at least one of the examined parameters. A principal component analysis showed that the enzyme ascorbate peroxidase can be an indicator of oxidative stress caused by ozone. By using oxidative stress indicators, it is possible to determine whether the trees are under stress even before visual damage occurs.

The Eastern Adriatic region, encompassing Croatia and Bosnia and Herzegovina, is considered an important area of natural populations of wild grapevines (Vitis vinifera ssp. sylvestris). The wild grapevine arises in the Eastern Adriatic region in a contact zone of the EU-Mediterranean and the sub-Mediterranean characterized by typical karst relief. This study focuses on the chloroplast DNA (cpDNA) analysis of wild grapevines and the biodiversity of accompanying woody species to better understand the genetic variation of the sylvestris populations of the Eastern Adriatic region and to investigate how this variation fits within today's wild grapevine distribution in the European continent. The allelic variation at nine cpDNA microsatellite loci of wild individuals was used to characterize haplotype diversity in 53 individuals from four population sites. All individuals were grouped into two chlorotypes: A and D, D being the rare haplotype among wild populations on the European continent. In total, 52 woody plant species were identified. However, the studied vegetation structures have been affected by permanent human pressure on natural resources and the preservation status of the collection sites. Based on our results, we conclude that the investigated areas were probably shelter zones for wild grapevine preservation during the unfavorable glaciation era.

The genetic diversity and relationship between wild (Vitis vinifera L. subsp. sylvestris (Gmel.) Hegi and cultivated (V. vinifera L. subsp. vinifera) grapevine in the western Balkan region and Central Europe have not been studied together previously, although this area has a rich viticultural past. Here, we studied wild grapevine populations sampled from their natural habitats in several countries of the western Balkan region and Central Europe. Their genetic diversity and structure were compared to cultivars that are traditionally in use in this region. A sample set of 243 accessions was genotyped at 20 nuclear microsatellite loci, including 167 sylvestris and 76 diverse vinifera cultivars. The genetic diversity of the wild grapevines was lower than that of cultivars by all genetic parameters. Both hierarchical and nonhierarchical clustering methods differentiated two main groups, indicating clear separation between wild and cultivated vines but also revealed clear gene flow between the cultivated and wild gene pools through overlaps and admixed ancestry values in the graphs. There was greater affinity to the wild grapes in Central European cultivars than in Balkan cultivars. Fine arrangement of the structure among cultivated grapevines showed differentiation among Central European and Balkan cultivars. These results confirm the divergence of wild grapes from vinifera and highlight the “crossroad” role of the western Balkan peninsula in the broader context of European viticulture.

Considering the fragility of the Mediterranean environment, there is an increasing need to improve the knowledge of this forest environment. The aim of this study was to examine the effects of air pollution on the forest ecosystem’s condition by analyzing tree vitality. The study area was chosen to represent the most important and the most common species in Mediterranean forest ecosystems of the Eastern Adriatic coast. Quercus pubescens, Quercus ilex, Pinus halepensis, and Pinus nigra plots were equipped with rain collectors and dendrometer bands. Sampling, measurements, and analyses of atmospheric deposition, foliar nutrient, defoliation, and growth were all carried out. Results showed that actual N deposition loads were the lowest in Aleppo pine forest and the highest in holm oak forests. This, however, did not have an effect on the concentrations of N in foliage. Most elements’ concentrations were in the plausible range. No relevant differences in mean defoliation between the plots were observed. The plots with a lower percentage of basal area increment (BAI%) were found to have lower defoliation. The research was conducted to bridge the gap in the knowledge of air pollutants and vitality indicators in different forest types. These findings are a valuable contribution to the sustainable forest management of Mediterranean forest.

This paper presents the results of research into the effects of stands of black pine (Pinus nigra A r n.) and Aleppo pine (Pinus halepensis M i l l.) on the protection of soil from rain-induced erosion. Research was conducted in the watershed of the torrent Suvava at Muć in experimental plots B4 and B5 inhabited by old stands of black pine at an inclination of 32°, and in the watershed of the torrent Rupotine near Solin in experimental plots B8 and B9 containing preserved and burnt stands of Aleppo pine at inclinations of 26 and 20°. The estimated mean annual values of surface runoff in old and open stands of black pine with completely preserved humus accumulative horizon covered with thick grass, at an inclination of 32 degree (plots B4), were 16.17 mm per square m (161.7 cubic m per ha), with the runoff coefficient of 0.015 and soil loss of 0.0107 t per ha. Surface runoff in fully canopied stands of black pine and no grass cover, at an inclination of 32 degree (plot B5), amounts to 31.65 mm per square m (316.1 cubic m per ha), with the runoff coefficient of 0.027 and soil loss of 0.0196 t per ha. In the fully canopied preserved stands of Aleppo pine, at an inclination of 26 degree (plot B8), surface runoff amounts to 6.23 mm per square m (62.3 cubic m per ha), with the degree (plot B9), surface runoff amounts to 35.51 mm per square m (355.1 cubic m per ha), with the runoff coefficient of 0.0478 and soil loss of 19.93 t per ha. According to the results, erosion-induced soil loss in the burnt forest area at an inclination of 20 degree is 463 times greater than under the preserved stands of Aleppo pine at an inclination of 26 degree.