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The re-excavation of Karnatukul (Serpent's Glen) has provided evidence for the human occupation of the Australian Western Desert to before 47,830 cal. BP (modelled median age). This new sequence is 20,000 years older than the previous known age for occupation at this site. Re-excavation of Karnatukul aimed to contextualise the site's painted art assemblage. We report on analyses of assemblages of stone artefacts and pigment art, pigment fragments, anthracology, new radiocarbon dates and detailed sediment analyses. Combined these add significantly to our understanding of this earliest occupation of Australia's Western Desert. The large lithic assemblage of over 25,000 artefacts includes a symmetrical geometric backed artefact dated to 45,570-41,650 cal. BP. The assemblage includes other evidence for hafting technology in its earliest phase of occupation. This research recalibrates the earliest Pleistocene occupation of Australia's desert core and confirms that people remained in this part of the arid zone during the Last Glacial Maximum. Changes in occupation intensity are demonstrated throughout the sequence: at the late Pleistocene/Holocene transition, the mid-Holocene and then during the last millennium. Karnatukul documents intensive site use with a range of occupation activities and different signalling behaviours during the last 1,000 years. This correlation of rock art and occupation evidence refines our understanding of how Western Desert peoples have inscribed their landscapes in the recent past, while the newly described occupation sequence highlights the dynamic adaptive culture of the first Australians, supporting arguments for their rapid very early migration from the coasts and northern tropics throughout the arid interior of the continent.

Anthracology (charcoal analysis) can inform about palaeoenvironments and human choices concerning the use of wood resources. While charcoal is commonly recovered during excavations, anthracology is poorly developed in Australian archaeology. This paper presents the first application of anthracology in the Midwest of Western Australia, at the Weld-RS-0731 (WA Department of Aboriginal Affairs Site ID 28793) site in the Weld Range. It uses methodological approaches developed by European anthracologists but not previously applied to Australian charcoal assemblages. The diversity and frequency of taxa identified in the late Holocene Weld-RS-0731 charcoal assemblages correspond to known vegetation communities, similar to those found in the area today. Nevertheless, the assemblages' compositions demonstrate the targeting of specific habitats, as well as the purposeful selection and avoidance of certain taxa. Our results confirm that wood gathering was not a separate specialist activity, but likely occurred alongside other subsistence tasks.

The re-excavation of Karnatukul (Serpent's Glen) has provided evidence for the human occupation of the Australian Western Desert to before 47,830 cal. BP (modelled median age). This new sequence is 20,000 years older than the previous known age for occupation at this site. Re-excavation of Karnatukul aimed to contextualise the site's painted art assemblage. We report on analyses of assemblages of stone artefacts and pigment art, pigment fragments, anthracology, new radiocarbon dates and detailed sediment analyses. Combined these add significantly to our understanding of this earliest occupation of Australia's Western Desert. The large lithic assemblage of over 25,000 artefacts includes a symmetrical geometric backed artefact dated to 45,570-41,650 cal. BP. The assemblage includes other evidence for hafting technology in its earliest phase of occupation. This research recalibrates the earliest Pleistocene occupation of Australia's desert core and confirms that people remained in this part of the arid zone during the Last Glacial Maximum. Changes in occupation intensity are demonstrated throughout the sequence: at the late Pleistocene/Holocene transition, the mid-Holocene and then during the last millennium. Karnatukul documents intensive site use with a range of occupation activities and different signalling behaviours during the last 1,000 years. This correlation of rock art and occupation evidence refines our understanding of how Western Desert peoples have inscribed their landscapes in the recent past, while the newly described occupation sequence highlights the dynamic adaptive culture of the first Australians, supporting arguments for their rapid very early migration from the coasts and northern tropics throughout the arid interior of the continent.

While archaeobotany is increasingly part of archaeological projects in Oceania, the specific sub-discipline focusing on wood charcoal macro-remains (anthracology) continues to be a much underdeveloped field of research in Australia and the Pacific. To initiate a regional framework for anthracology, we present here a review of studies based on wood charcoal analyses that have been implemented in Oceania, and we then present anthracological principles and methods developed in other parts of the world. We use three recent case studies, from New Caledonia, and tropical and semi-arid Australia, to illustrate the application of anthracological methods in the region. Finally, we consider the potential for the discipline to be successfully developed in Oceania, discussing identified challenges and prospects for anthracology to address key archaeological questions in the region. We argue the discipline has the potential to throw light on both palaeoenvironmental conditions and palaeoethnobotanical practices at a site, and can also offer insights in relation to mobility patterns and resource management in the past.

Anthracology (charcoal analysis) can inform about palaeoenvironments and human choices concerning the use of wood resources. While charcoal is commonly recovered during excavations, anthracology is poorly developed in Australian archaeology. This paper presents the first application of anthracology in the Midwest of Western Australia, at the Weld-RS-0731 (WA Department of Aboriginal Affairs Site ID 28793) site in the Weld Range. It uses methodological approaches developed by European anthracologists but not previously applied to Australian charcoal assemblages. The diversity andfrequency of taxa identified in the late Holocene Weld-RS-0731 charcoal assemblages correspond to known vegetation communities, similar to those found in the area today. Nevertheless, the assemblages' compositions demonstrate the targeting of specific habitats, as well as the purposeful selection and avoidance of certain taxa. Our results confirm that wood gathering was not a separate specialist activity, but likely occurred alongside other subsistence tasks.

The establishment of north-west Australia’s nineteenth-century pearlshell fisheries led to the first occupation of many arid offshore islands since the early mid-Holocene. The nature of this occupation, and how crews subsisted on such remote landscapes, remains poorly understood. We investigate a rare instance of an archaeobotanical and zooarchaeological record for this colonial-era activity at Bandicoot Bay, Barrow Island. Varied taxonomic representation suggests an atypical subsistence strategy for a maritime industry, involving broad exploitation of resources over several local landscapes and an absence of remains of provisioned food. Identified plant taxa largely conform to resources known ethnographically to have been exploited by north-west Aboriginal communities, and many relate to traditional food practices. The spatial patterning of charred wood and bone suggests multiple burning activities and areas of specific use. Conditions of wood fuel on the island may have prompted the augmentation of fuel, indicated by the presence of non-local wood charcoal and high proportions of calcined bone. These findings (i) are consistent with the hypothesis that the site’s occupants originated from the north-west as part of coerced pearling labour and (ii) provide unique insight into the role offshore islands may have occupied in the management of this industry’s labour forces. Au 19e siècle, le développement d’une industrie de pêche des huitres perlières dans le nord-ouest de l’Australie entraina la première réoccupation de nombreuses îles arides de cette région, après celles datant du milieu de l’Holocène. La nature de ces occupations et la façon dont les équipages purent subsister au sein de milieux si isolés demeurent mal comprises. Nous examinons ici un exemple rare d’assemblages archéozoologiques et archéobotaniques pour ce type d’activités de la période coloniale, dans la baie de Bandicoot, Barrow Island. La diversité des représentations taxonomiques de faune suggère une stratégie de subsistance atypique pour une industrie maritime, incluant un mode d’exploitation extensif des ressources issues de plusieurs environnements locaux, ainsi qu’une absence de restes provenant de nourriture approvisionnée. Les taxons botaniques identifiés correspondent à ceux exploités par les communautés aborigènes du nord-ouest du continent, selon les données ethnographiques, et beaucoup sont associés aux pratiques de subsistance traditionnelles. La répartition spatiale des restes anthracologiques et de faune suggère l’existence de plusieurs types de combustion et zones d’activités spécialisées. La disponibilité du bois de feu sur l’île a pu motiver la recherche de solutions pour augmenter les ressources en combustible, comme semble l’indiquer la présence de charbons de bois d’essence non locale et les proportions importantes d’os calcinés. Ces résultats (i) supportent l’hypothèse selon laquelle les occupants de ce site étaient originaires du nord-ouest du continent et furent déplacés par le biais du système de travail forcé imposé par l’industrie perlière, et (ii) offrent un aperçu unique de la façon dont cette industrie utilisa l’isolement de certaines îles pour gérer sa main d’œuvre sous contrainte.

While archaeobotany is increasingly part of archaeological projects in O ceania, the specific sub‐discipline focusing on wood charcoal macro‐remains (anthracology) continues to be a much underdeveloped field of research in A ustralia and the P acific. To initiate a regional framework for anthracology, we present here a review of studies based on wood charcoal analyses that have been implemented in O ceania, and we then present anthracological principles and methods developed in other parts of the world. We use three recent case studies, from N ew C aledonia, and tropical and semi‐arid A ustralia, to illustrate the application of anthracological methods in the region. Finally, we consider the potential for the discipline to be successfully developed in O ceania, discussing identified challenges and prospects for anthracology to address key archaeological questions in the region. We argue the discipline has the potential to throw light on both palaeoenvironmental conditions and palaeoethnobotanical practices at a site, and can also offer insights in relation to mobility patterns and resource management in the past.

Readily synthesized maltose–neopentyl glycol (MNG) amphiphiles are useful reagents for stabilizing, extracting and crystallizing a variety of integral membrane proteins and have favorable properties relative to conventional detergents. The understanding of integral membrane protein (IMP) structure and function is hampered by the difficulty of handling these proteins. Aqueous solubilization, necessary for many types of biophysical analysis, generally requires a detergent to shield the large lipophilic surfaces of native IMPs. Many proteins remain difficult to study owing to a lack of suitable detergents. We introduce a class of amphiphiles, each built around a central quaternary carbon atom derived from neopentyl glycol, with hydrophilic groups derived from maltose. Representatives of this maltose–neopentyl glycol (MNG) amphiphile family show favorable behavior relative to conventional detergents, as manifested in multiple membrane protein systems, leading to enhanced structural stability and successful crystallization. MNG amphiphiles are promising tools for membrane protein science because of the ease with which they may be prepared and the facility with which their structures may be varied.

High-resolution membrane protein structures are essential for understanding the molecular basis of diverse biological events and important in drug development. Detergents are usually used to extract these bio-macromolecules from the membranes and maintain them in a soluble and stable state in aqueous solutions for downstream characterization. However, many eukaryotic membrane proteins solubilized in conventional detergents tend to undergo structural degradation, necessitating the development of new amphiphilic agents with enhanced properties. In this study, we designed and synthesized a novel class of glucoside amphiphiles, designated tandem malonate-based glucosides (TMGs). A few TMG agents proved effective at both stabilizing a range of membrane proteins and extracting proteins from the membrane environment. These favourable characteristics, along with synthetic convenience, indicate that these agents have potential in membrane protein research.